本文選題：非酒精性脂肪肝病 + 卡托普利��； 參考：《瀘州醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的：觀察卡托普利對非酒精性脂肪肝大鼠的治療效果并探討其作用機制。方法：將80只雄性SD大鼠按1：5比例隨機分為2組：正常對照組與高脂組。除第1周外,整個喂養(yǎng)期間,正常對照組大鼠給予普通飼料,其余大鼠給予高脂飲食(普通飼料+10%豬油+2%膽固醇+0.2%膽酸鈉),所有大鼠自由飲水和進食。造模6周后,將高脂組隨機分為5組：模型對照組、卡托普利高、中、低劑量組和羅格列酮陽性對照組。分別給予相應(yīng)大鼠等容量羅格列酮和高、中、低劑量卡托普利,給予正常對照組和模型對照組等容量蒸餾水,每天灌胃一次。給藥6周后,所有大鼠禁食12小時,稱重,用1%戊巴比妥鈉(0.4 ml·100g-1)進行麻醉后處死。腹主動脈取血,用全自動生化分析儀檢測其血清空腹血糖(Fasting blood glucose, FBG)、丙氨酸氨基轉(zhuǎn)移酶(Alanine aminotransferase、天門冬氨酸氨基轉(zhuǎn)移酶(Aspartate aminotransferase, AST)、總膽固醇(Total cholesterol, TC)、甘油三酯(Triglyceride, TG)水平；放射免疫分析法(Radioimmunoassay, RIA)檢測血清空腹胰島素(fasting insulin, INS)水平；硫代巴比妥酸(Thibabituric acid, TBA)法檢測血清丙二醛(Malondialdehyde, MDA)水平、比色法檢測血清谷胱甘肽(Glutathione, GSH)水平；酶聯(lián)免疫吸附測定法(Enzyme-linked immunosorbent assay, ELISA)檢測血清瘦素(Leptin, LEP)水平。分離肝臟并稱量肝濕重,然后取相同部位肝組織作HE染色,于光鏡下觀察其病理學(xué)變化；取另一部分肝臟進行組織勻漿,采用酶偶聯(lián)比色法檢測其TC、TG含量。采用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)(Reverse transcription-polymerase chain reaction, RT-PCR)技術(shù)檢測肝組織中CYP2E1的mRNA表達水平。按公式計算胰島素抵抗指數(shù)(Insulin resistance index, IRI)、胰島素敏感指數(shù)(Insulin sensitive index, ISI)和肝指數(shù)(Liver index)。結(jié)果：模型對照組FBG、INS、TC、TG、LEP、ALT、AST、MDA含量、肝指數(shù)、IRI、肝組織CYP2E1的mRNA表達水平均增高,GSH含量和ISI下降,與正常對照組比較,有顯著性差異(P0.01或P0.05)�？ㄍ衅绽邉┝拷MFBG、INS、TC、TG、LEP、ALT、AST、MDA含量、肝指數(shù)、IRI及肝組織CYP2E1的mRNA表達水平均降低,GSH含量和ISI上升,與模型對照組比較,有顯著性差異(P0.01或P0.05)；陽性對照組FBG、INS、TC、TG、LEP、MDA含量、肝指數(shù)、IRI及肝組織CYP2E1的mRNA表達水平均降低,ISI上升,與模型對照組比較,有顯著性差異(P0.01或P0.05)。肝組織光學(xué)顯微鏡下病理學(xué)觀察發(fā)現(xiàn)：模型對照組可見彌漫性脂肪變性,主要為大泡性脂肪變性,肝細胞水腫、細胞排列紊亂,并可見大量炎細胞浸潤�？ㄍ衅绽邉┝拷M可見少量脂肪變性,主要為小泡性脂肪變性。大多數(shù)肝細胞排列正常,部分細胞水腫,并可見少量炎細胞浸潤。陽性對照組病理變化情況與卡托普利高劑量組相似,只是脂肪變性較多且有部分為大泡性脂肪變性。結(jié)論：非酒精性脂肪肝大鼠造模成功。卡托普利能減輕肝細胞脂肪變性,對非酒精性脂肪肝有一定的防治作用。卡托普利可通過改善機體胰島素抵抗、增強其抗氧化應(yīng)激能力而達到防治效果。
[Abstract]:Objective: To observe the therapeutic effect of Kato Pury on nonalcoholic fatty liver in rats and explore its mechanism. Methods: 80 male SD rats by 1:5 were randomly divided into 2 groups: normal control group and hyperlipidemia group. In first weeks, the whole feeding period, the rats in the normal control group received general feed, the remaining rats were given high fat diet (normal diet +10% lard +2% cholesterol +0.2% sodium cholate), free drinking and eating all the rats. After 6 weeks, high fat diet group were divided into 5 groups: model control group, Kato Pury high, low dose group and rosiglitazone positive control group respectively. Give the corresponding rat capacity of rosiglitazone and high, low dose of Kato Pury, given the normal control group and model control group were treated with equal volume of distilled water by gavage once a day. After 6 weeks, all rats were fasted for 12 hours, weighing, with 1% pentobarbital sodium (0.4 ml 100g-1) of Ma Drunk. After killing the blood from the abdominal aorta, the serum fasting blood glucose was detected by automatic biochemical analyzer (Fasting blood, glucose, FBG), Alanine aminotransferase (alanine aminotransferase, aspartate aminotransferase (Aspartate, aminotransferase, AST), total cholesterol (Total, cholesterol, TC), triglycerides (Triglyceride, TG) level; radioimmunoassay (Radioimmunoassay, RIA) detection of serum fasting insulin (fasting, insulin, INS) level of thiobarbituric acid (Thibabituric; acid, TBA) were used to detect the serum malondialdehyde (Malondialdehyde, MDA) level, serum glutathione ratio method (Glutathione, GSH); enzyme linked immunosorbent assay (Enzyme-linked immunosorbent assay, ELISA) detection of serum leptin (Leptin, LEP). The level of separating the liver and liver wet weight weighing, and then take the liver tissue HE staining, light To observe the pathological changes under microscope; another part of the liver tissue homogenate, colorimetric method was used to detect the TC enzyme coupling TG content. Using reverse transcriptase polymerase chain reaction (Reverse transcription-polymerase chain reaction, RT-PCR) the expression level of CYP2E1 in liver tissue were detected in mRNA. According to the formula for the calculation of insulin resistance index (Insulin resistance index, IRI), insulin sensitivity index (Insulin sensitive, index, ISI) and liver index (Liver index). Results: the model control group FBG, INS, TC, TG, LEP, ALT, AST, MDA content, liver index, IRI, the expression level of CYP2E1 in liver tissue of mRNA were increased, the content of GSH and ISI decreased, compared with normal control group, there was significant difference (P0.01 or P0.05). Kato Pury Gogh FBG INS, TC group, TG, LEP, ALT, AST, MDA content, liver index, the expression level of IRI and CYP2E1 in liver tissues of mRNA were decreased and the content of GSH and ISI increased, and The model group, there was significant difference (P0.01 or P0.05); positive control group FBG, INS, TC, TG, LEP, MDA content, liver index, the expression level of IRI and CYP2E1 in liver tissues of mRNA were decreased, ISI increased, compared with the model group, there was significant difference (P0.01 or P0.05) observation of optical microscope. Liver tissue pathological model control group showed diffuse fatty degeneration, mainly for bullous steatosis, liver cell edema, cell disorder, and the number of inflammatory cell infiltration. Kato Pury Gogh dose group showed a small amount of fat, mainly for microvesicular steatosis. Most liver cells were normal. Some cells edema, and a small amount of inflammatory cell infiltration. Positive control group pathological changes and Kato Pury Gogh dose were similar, only more fatty degeneration and partial bullous steatosis. Conclusion: nonalcoholic fatty liver disease in rats Kato Pury can reduce the steatosis of liver cells and have some preventive and therapeutic effects on non-alcoholic fatty liver disease. Kato Pury can improve the body's insulin resistance and enhance its ability of anti oxidative stress, so as to achieve the control effect.

【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965

【相似文獻】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營養(yǎng)學(xué)報;1957年04期

2 陳偉強;趙善廣;;自制注射用大鼠固定裝置[J];上海實驗動物科學(xué);1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報;2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實驗動物科學(xué);2001年03期

6 戴英,陸群;復(fù)方H_(505)對Wistar大鼠外周血的血液流變學(xué)指標的影響[J];中國血液流變學(xué)雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長斌;+Gz暴露時間對大鼠記憶功能和行為的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2003年01期

8 呂學(xué)軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國職業(yè)醫(yī)學(xué);2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學(xué)院學(xué)報;2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡易的大鼠保定方法[J];上海實驗動物科學(xué);2004年01期

相關(guān)會議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：1732822