本文選題：微球　切入點(diǎn)：顯微結(jié)構(gòu)　出處：《中山大學(xué)學(xué)報(bào)(自然科學(xué)版)》2015年03期

【摘要】：采用復(fù)乳法制備載牛血清白蛋白的聚乳酸聚乙醇酸(PLGA)微球,研究初乳攪拌速率(5 000、10 000和15 000 r/min)對(duì)微球性質(zhì)的影響。以包封率、載藥量、粒徑等為指標(biāo),評(píng)價(jià)其理化性質(zhì);掃描電鏡觀察微球內(nèi)外形態(tài),并通過圖像分析軟件計(jì)算其結(jié)構(gòu)參數(shù);激光共聚焦顯微鏡觀察蛋白在微球表面及骨架內(nèi)的分布情況;熒光攝取實(shí)驗(yàn)考察孔洞與微球表面連通情況;并考察微球體外釋藥行為及降解過程中微球的內(nèi)外形態(tài)變化。隨著攪拌速率的增加,微球表面及內(nèi)部孔洞增加,內(nèi)部孔洞孔徑減小,微球釋藥及降解速率減慢,但對(duì)微球粒徑及截面孔隙率無顯著影響。10 000 r/min組,微球包封率有最大值,1 d的突釋率最低,且有最低的表面孔洞連通率;5 000 r/min和15 000 r/min組,微球突釋率較高。不同初乳攪拌速率所得微球的內(nèi)外形態(tài)在載蛋白的PLGA微球的釋放中發(fā)揮重要作用。
[Abstract]:Poly (Polylactic acid-poly (ethanolide)) PLGA microspheres loaded with bovine serum albumin (BSA) were prepared by double milk method. The effects of the mixing rates of colostrum (5 000 ~ 10 000 and 15 000 r / min) on the properties of the microspheres were studied.The physical and chemical properties of the microspheres were evaluated by the encapsulation efficiency, drug loading and particle size, the morphology of the microspheres was observed by scanning electron microscope, and the structural parameters were calculated by image analysis software.The distribution of protein on the surface and skeleton of the microspheres was observed by laser confocal microscopy. The connection between the pores and the microspheres was investigated by fluorescence uptake. The in vitro drug release behavior of the microspheres and the morphological changes of the microspheres in the process of degradation were also investigated.With the increase of stirring rate, the surface and internal pores of the microspheres increased, the pore size of the microspheres decreased, the drug release and degradation rate of the microspheres slowed down, but there was no significant effect on the particle size and the cross section porosity of the microspheres.When the encapsulation efficiency of the microspheres was the highest, the initial release rate of the microspheres was the lowest, and there were the lowest surface cavity connectivity of 5 000 r/min and 15 000 r/min groups, and the sudden release rate of the microspheres was higher than that of the group of 5 000 r/min and 15 000 r/min.The internal and external morphology of the microspheres at different rates of colostrum stirring played an important role in the release of protein-loaded PLGA microspheres.
【作者單位】： 中山大學(xué)附屬第三醫(yī)院;中山大學(xué)藥學(xué)院;
【基金】：廣東省重大專項(xiàng)科技計(jì)劃資助項(xiàng)目(2011A080504003)
【分類號(hào)】：R943

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本文編號(hào)：1730609