本文選題：咖啡因　切入點：茶堿　出處：《藥物分析雜志》2016年12期

【摘要】：目的:建立反相高效液相色譜法同時測定早產(chǎn)兒血清中咖啡因和茶堿的濃度,用于血藥濃度監(jiān)測,防范不良反應的發(fā)生,并將其應用于咖啡因在早產(chǎn)兒的群體藥代動力學研究。方法:以二羥丙茶堿為內(nèi)標,血清樣品用乙酸乙酯-二氯甲烷(4∶1)提取后,反相高效液相色譜法檢測咖啡因和茶堿的濃度。色譜條件:采用ZORBAX SB-C18(150 mm×4.6 mm,5μm)色譜柱,以甲醇-0.2%醋酸溶液(25∶75)為流動相,流速1 m L·min~(-1),紫外檢測波長273 nm,柱溫40℃。結(jié)果:咖啡因和茶堿的相對回收率范圍分別為99.4%~107.7%和100.2%~114.0%,提取回收率范圍分別為69.2%~73.0%和50.5%~54.4%;咖啡因和茶堿的批內(nèi)RSD分別為2.2%~4.9%和1.7%~4.7%,批間RSD分別為2.5%~6.7%和1.9%~5.3%。10例早產(chǎn)兒的咖啡因血藥濃度為(15.6±6.6)μg·m L~(-1),茶堿血藥濃度(0.8±0.1)μg·m L~(-1),未超出本方法的檢測范圍,也未發(fā)生不良反應。結(jié)論:本法經(jīng)方法學驗證,適用于早產(chǎn)兒咖啡因藥代動力學研究。
[Abstract]:Objective: to establish a reversed phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of caffeine and theophylline in serum of premature infants.Methods: the concentration of caffeine and theophylline was determined by reversed-phase high performance liquid chromatography (RP-HPLC) with dihydroxypropylline as internal standard and serum samples extracted with ethyl acetate-dichloromethane (4: 1).The chromatographic conditions were as follows: ZORBAX SB-C18(150 mm 脳 4.6 mm ~ (5 渭 m) column, methanol-0.2% acetic acid solution (25: 75) as mobile phase, flow rate 1 mL 路min ~ (-1), UV detection wavelength 273 nm, column temperature 40 鈩，

本文編號：1719086