本文選題：注射用三重霉素　切入點(diǎn)：普魯卡因　出處：《中國藥房》2017年03期

【摘要】：目的:建立同時(shí)測定注射用三重霉素中普魯卡因、二芐基乙二胺、青霉素含量的方法。方法:采用高效液相色譜法。色譜柱為Sino Chrom C_(18),流動相為0.21 mol/L磷酸二氫鉀溶液(含0.16%的三乙胺,氫氧化鉀溶液調(diào)pH至5.6)-乙腈(81∶19,V/V),流速為1.0 mL/min,檢測波長為215 nm,柱溫為25℃,進(jìn)樣量為10μL。結(jié)果:普魯卡因、二芐基乙二胺、青霉素檢測質(zhì)量濃度線性范圍分別為0.011~0.64 mg/mL(r=0.999 9)、0.013~0.80 mg/mL(r=0.999 9)、0.075~4.50 mg/mL(r=0.999 9);精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD0.70%;加樣回收率分別為98.42%~101.51%(RSD=0.90%,n=9)、98.28%~101.50%(RSD=0.90%,n=9)、99.03%~101.11%(RSD=0.66%,n=9)。結(jié)論:該方法簡便、準(zhǔn)確,可用于注射用三重霉素中普魯卡因、二芐基乙二胺和青霉素含量的同時(shí)測定。
[Abstract]:Objective : To establish a method for simultaneous determination of proparacaine , dibenzyl ethylenediamine and penicillin in injection for injection . The chromatographic column was Sino Chrom C _ ( 18 ) , the mobile phase was 0.21 mol / L potassium dihydrogen phosphate solution ( containing 0.16 % triethylamine , potassium hydroxide solution adjusted to 5.6 ) - acetonitrile ( 81 : 19 , V / V ) , the flow rate was 1.0 mL / min , the detection wavelength was 215 nm , the column temperature was 25 鈩，

本文編號：1717084