本文選題：丙戊酸鈉　切入點：血藥濃度　出處：《中國醫(yī)院藥學雜志》2017年13期

【摘要】：目的:分析丙戊酸鈉血藥濃度測定衍生時的影響因素,保證臨床測定的準確性。方法:丙戊酸鈉血藥濃度用高效液相色譜(HPLC)法測定。血漿樣品用2-溴-對硝基苯乙酮為衍生試劑,以丙戊酸鈉衍生物產(chǎn)率為指標,用單因素考察法考察衍生試劑濃度、三乙胺體積分數(shù)、反應(yīng)溫度、反應(yīng)時間對丙戊酸鈉衍生產(chǎn)率的影響。結(jié)果:衍生試劑2-溴-對硝基苯乙酮濃度為1.5~2.0mg·mL~(-1),催化劑三乙胺體積分數(shù)為20%,衍生溫度和衍生時間分別為60℃,10 min時,丙戊酸衍生完全,血藥濃度在10~200μg·mL~(-1)范圍內(nèi),線性較好(R~2=0.999 5),回收率為95.00%~100.59%,日內(nèi)與日間RSD≤5%,符合《中國藥典》中對生物樣本藥物濃度檢測的規(guī)定。結(jié)論:影響丙戊酸血藥濃度測定的因素較多,如衍生試劑濃度和三乙胺體積分數(shù)、衍生時間、衍生溫度等。在日常血藥濃度監(jiān)測過程中,我們應(yīng)注意這些差異,盡量將誤差控制在允許范圍之內(nèi),保證丙戊酸鈉治療藥物監(jiān)測結(jié)果的準確性。
[Abstract]:Objective: to analyze the factors influencing the determination of valproate concentration in blood and to ensure the accuracy of clinical determination.Methods: the concentration of sodium valproate in blood was determined by high performance liquid chromatography (HPLC).The concentration of derivative reagent, the volume fraction of triethylamine, the reaction temperature and the yield of sodium valproate derivatives were investigated by single factor method with 2-bromo-p-nitroacetophenone as derivative reagent and sodium valproate derivative yield as index.The effect of reaction time on the productivity of sodium valproate.Results: when the concentration of 2-bromo-p-nitroacetophenone was 1.5~2.0mg mLL-1, the volume fraction of triethylamine was 20, and the derivatization temperature and time were 60 鈩，

本文編號：1709269