本文選題：單寧酸　切入點(diǎn)：順鉑　出處：《天然產(chǎn)物研究與開發(fā)》2017年11期

【摘要】：本文主要研究單寧酸(TA)聯(lián)合順鉑(CDDP)對肝癌HepG2細(xì)胞內(nèi)質(zhì)網(wǎng)應(yīng)激IRE1-XBP1通路的影響。用180μM單寧酸、0.9μg/m L順鉑單獨(dú)用藥或者聯(lián)合用藥處理肝癌HepG2細(xì)胞24 h或48 h后,應(yīng)用流式細(xì)胞技術(shù)測定HepG2細(xì)胞的凋亡率,實(shí)時(shí)熒光定量PCR技術(shù)(q-RT-PCR)、蛋白免疫印跡(western blot)技術(shù)檢測IRE1α和XBP-1分子的表達(dá)水平。MTT結(jié)果顯示,單寧酸和順鉑均能顯著抑制HepG2細(xì)胞的生長,且均呈劑量性依賴;二者聯(lián)合用藥能夠顯著增加HepG2細(xì)胞的生長抑制率;流式細(xì)胞術(shù)結(jié)果顯示,單寧酸與順鉑聯(lián)合用藥能夠顯著抑制HepG2細(xì)胞的增殖,并誘導(dǎo)細(xì)胞凋亡的發(fā)生;q-RT-PCR及Western blot結(jié)果顯示,單寧酸與順鉑聯(lián)合用藥能顯著上調(diào)細(xì)胞IRE1α和XBP-1的表達(dá)水平。結(jié)果表明單寧酸能夠聯(lián)合順鉑增強(qiáng)肝癌HepG2細(xì)胞內(nèi)質(zhì)網(wǎng)應(yīng)激IRE1-XBP1通路的激活水平,提示IRE1-XBP1通路可能是單寧酸和順鉑協(xié)同抗肝癌HepG2細(xì)胞的分子機(jī)制之一。
[Abstract]:In this study, we studied the effects of Tannin combined with cisplatin CDDPon on endoplasmic reticulum stress (IRE1-XBP1) pathway in hepatocellular carcinoma (HepG2) cells.The expression levels of IRE1 偽 and XBP-1 molecules were detected by quantitative real-time PCR and Western blot. The results showed that both tannic acid and cisplatin could significantly inhibit the growth of HepG2 cells in a dose-dependent manner.The results of flow cytometry showed that the combination of tannic acid and cisplatin could significantly inhibit the proliferation of HepG2 cells, and induce apoptosis of HepG2 cells by q-RT-PCR and Western blot.Tannic acid combined with cisplatin could significantly up-regulate the expression of IRE1 偽 and XBP-1.The results showed that tannic acid combined with cisplatin could enhance the activation level of endoplasmic reticulum stress IRE1-XBP1 pathway in HepG2 cells, suggesting that the IRE1-XBP1 pathway might be one of the molecular mechanisms of synergistic effect of tannic acid and cisplatin on hepatoma HepG2 cells.
【作者單位】： 遵義醫(yī)學(xué)院口腔學(xué)院;遵義醫(yī)學(xué)院醫(yī)學(xué)與生物學(xué)研究中心貴州省普通高等學(xué)校口腔疾病研究特色重點(diǎn)實(shí)驗(yàn)室;遵義醫(yī)學(xué)院醫(yī)學(xué)遺傳學(xué)教研室;
【基金】：貴州省教育廳特色藥用資源研發(fā)創(chuàng)新團(tuán)隊(duì)(2013-15) 貴州省科技廳資助(2014-7548) 遵義醫(yī)學(xué)院碩士啟動基金(F-841);遵義醫(yī)學(xué)院碩士啟動基金(F-827)
【分類號】：R96
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本文編號：1696275