發(fā)布時(shí)間：2018-04-01 09:25

  本文選題：紅樹林　切入點(diǎn)：內(nèi)生放線菌　出處：《中國抗生素雜志》2017年04期

【摘要】：目的研究澳門紅樹林植物內(nèi)生放線菌多樣性及新穎性,為放線菌新物種及新抗生素發(fā)現(xiàn)奠定基礎(chǔ)。方法 12份采集自澳門紅樹林的植物樣品,消毒粉碎后,撒在10種分離培養(yǎng)基上分離內(nèi)生放線菌,采用ISP2培養(yǎng)基進(jìn)行菌株純化;通過PCR擴(kuò)增和測(cè)序獲得16S r RNA基因序列,并提交到數(shù)據(jù)庫中進(jìn)行搜索比對(duì),開展內(nèi)生放線菌多樣性和新穎性分析;放線菌潛在新物種經(jīng)液體發(fā)酵、離心后,上清液經(jīng)乙酸乙酯萃取,菌絲經(jīng)丙酮浸泡,分別獲得發(fā)酵液水相樣品、發(fā)酵液酯相樣品和菌絲丙酮浸提液3類樣品;樣品通過紙片擴(kuò)散法進(jìn)行抗菌活性初篩。結(jié)果從12份植物樣品中得到192株內(nèi)生放線菌,分布于8個(gè)目17個(gè)科30個(gè)屬,其中鏈霉菌為優(yōu)勢(shì)菌屬;16S r RNA基因序列相似性低于98.6%的菌株共有22株,其中菌株4Q3S-3和2Q3S-4-2分別為弗萊德門菌屬(Friedmanniella)和中村菌屬(Nakamurella)的新種,放線菌潛在新物種的鑒定工作正在進(jìn)行;2株放線菌新種及6株潛在放線菌新種的抗菌活性篩選結(jié)果表明,5株具有抗菌活性。結(jié)論澳門紅樹林植物內(nèi)生放線菌資源豐富多樣,新穎性高,具有從中發(fā)現(xiàn)放線菌新物種和新抗生素的潛力,值得深入研究。
[Abstract]:Objective to study the diversity and novelty of endophytic actinomycetes in mangrove plants in Macao, and to lay a foundation for the discovery of new species and antibiotics of actinomycetes. Endophytic actinomycetes were isolated from 10 kinds of culture medium and purified by ISP2 medium. The 16s r RNA gene sequence was obtained by PCR amplification and sequencing, and was submitted to the database for search and comparison. The diversity and novelty of endophytic actinomycetes were analyzed. After liquid fermentation, centrifugation, the supernatant was extracted by ethyl acetate, and the hyphae was soaked in acetone to obtain the water phase samples of fermentation broth. Results 192 strains of endophytic actinomycetes were obtained from 12 plant samples, which were distributed in 8 orders, 17 families, 30 genera, 8 orders, 17 families, 30 genera, 8 orders, 17 families, 30 genera, and 192 strains of endophytic actinomycetes were obtained from 12 plant samples. There were 22 strains in which the sequence similarity of 16s r RNA gene of Streptomyces was less than 98.6%, among which 4Q3S-3 and 2Q3S-4-2 were new species of Friedmanniella and Nakamurella, respectively. Two new actinomycetes and six new potential actinomycetes were screened for their antibacterial activities. Conclusion the endophytic actinomycetes resources of mangrove plants in Macao are rich and varied. It has the potential to find new species of actinomycetes and new antibiotics, which is worthy of further study.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院醫(yī)藥生物技術(shù)研究所;遵義醫(yī)學(xué)院;中國藥科大學(xué);澳門大學(xué);
【基金】：國家自然科學(xué)基金(No.81373308和No.81321004) 澳門大學(xué)研究基金(No.MYRG2016-00056-FST)
【分類號(hào)】：R915

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1 李靜;海南東寨港紅樹林植物內(nèi)生放線菌藥用資源勘探[D];北京協(xié)和醫(yī)學(xué)院;2016年

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本文編號(hào)：1694929