本文選題：仲鎢酸鹽　切入點：人卵巢癌細胞SKOV-　出處：《東北師大學報(自然科學版)》2017年03期

【摘要】：研究了過渡金屬銅修飾的仲鎢酸鹽化合物[Na_2(H_2O)_(10)][Cu_4(H_2O)_(12)(H_2W_(12)O_(42))]·15H_2O(CuW_(12))的體外抗腫瘤活性.取人卵巢癌細胞SKOV-3加入不同濃度CuW_(12)處理,應用四甲基偶氮唑鹽(MTT)比色法分析細胞增殖,計算了半數(shù)抑制濃度(IC_(50));采用光學顯微鏡觀察SKOV-3細胞的凋亡形態(tài)變化;分析了細胞周期和細胞凋亡,計算了各期細胞比例及細胞凋亡率.結(jié)果表明:CuW_(12)對SKOV-3細胞增殖抑制的IC_(50)值為45.2μmol/L,且呈劑量依賴性;與經(jīng)典的仲鎢酸鹽Na_(10)(H_2W_(12)O_(42))·26H_2O(NaW_(12))相比,過渡金屬銅修飾的仲鎢酸鹽抗腫瘤活性更強;光學顯微鏡下觀察到CuW_(12)處理組細胞數(shù)目明顯減少,出現(xiàn)變圓、縮小、老化等形態(tài)變化;不同濃度CuW_(12)處理12h后早期凋亡細胞所占的百分比顯著增加且呈劑量依賴性.
[Abstract]:The in vitro antitumor activity of the transition-metal copper modified paratungstate compound [Na _ 2H _ S _ T _ 2O _ 10] [Cu4H _ S _ 2O _ O _ T] [Cu4H _ S _ 2H _ 2O _ O _ T _ T _ (12 / S))] 15H _ 2O-Stam _ CuW _ (12) was studied, and the cell proliferation was analyzed by MTT colorimetric analysis by adding different concentrations of CuW _ (12) to the human ovarian cancer cell line SKOV-3. The half inhibitory concentration (ICS) was calculated, the morphological changes of SKOV-3 cells were observed by optical microscope, and the cell cycle and apoptosis were analyzed. The cell proportion and apoptosis rate of each phase were calculated. The results showed that the ICSP 50) value of the inhibition of SKOV-3 cell proliferation was 45.2 渭 mol / L in a dose-dependent manner, and the antitumor activity of commatungstate modified by transition metal copper was higher than that of the classical secatungstate Nastra (42D) 26H _ 2O NaW _ (12) (P < 0.05). It was observed under optical microscope that the number of cells in the cuw _ (12) treatment group was obviously decreased, and the morphological changes such as circle, reduction and aging were observed, and the percentage of early apoptotic cells increased significantly after 12 h treatment with different concentrations of CuW _ (12) in a dose-dependent manner.
【作者單位】： 海軍92538部隊醫(yī)院;吉林化工學院化學與制藥工程學院;哈爾濱商業(yè)大學旅游與烹飪學院;
【基金】：國家自然科學基金資助項目(21301041) 吉林省教育廳科學技術(shù)研究項目(2015431)
【分類號】：R96
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本文編號：1672967