本文選題：再灌注損傷　切入點(diǎn)：曲美他嗪　出處：《山東醫(yī)藥》2015年29期

【摘要】：目的觀察鹽酸曲美他嗪(TMZ)預(yù)處理對(duì)大鼠腎組織急性缺血再灌注損傷(IRI)的影響。方法選擇SD大鼠90只,隨機(jī)均分為A、B兩組:A組給予TMZ 10 mg/kg灌胃5 d后,建立缺血再灌注(IR1)、缺血再灌注后處理(IPO1)、假手術(shù)(S1)模型;B組生理鹽水灌胃5 d后,建立缺血再灌注(IR2)、缺血再灌注后處理(IPO2)、假手術(shù)(S2)模型。檢測(cè)各組末次恢復(fù)腎臟血供0、6、12、24、48 h后血清SCr、BUN、NGAL、IL-18水平;HE染色光鏡下觀察缺血-再灌注石蠟包埋切片腎組織損傷形態(tài)學(xué)改變。結(jié)果 IR1組與IPO1組相比,在各時(shí)間點(diǎn)各指標(biāo)均無(wú)顯著性差異。IR1組與IR2組相比,在6 h時(shí)IR2組的NGAL、IL-18、腎小管損傷程度評(píng)分上升,12 h時(shí)BUN亦上升,24 h時(shí)IR2組的各項(xiàng)指標(biāo)均高于IR1組,48 h時(shí)BUN、SCr高于IR1組(P均0.05)。IPO1組與IPO2組相比,在6 h時(shí)IPO兩組的NGAL明顯上升,12 h時(shí)除SCr外均上升,24 h時(shí)除NGAL外其他指標(biāo)均高于IPO1組,48 h時(shí)BUN、SCr、腎小管損傷評(píng)分程度高于IPO1組(P均0.05)。IR1組與IPO2組相比,在6 h時(shí)IPO2組NGAL上升,12 h時(shí)除SCr外其他指標(biāo)均高于IR1組,48 h時(shí)SCr仍顯著高于IR1組(P均0.05)。結(jié)論在大鼠腎臟IRI發(fā)生之前即預(yù)防性使用TMZ可減輕其腎功能損傷。
[Abstract]:Objective to observe the effect of trimetazidine hydrochloride (TMZ) preconditioning on acute ischemia-reperfusion injury (IRI) in renal tissue of rats. Methods 90 SD rats were randomly divided into two groups: group A (n = 10) and group A (n = 10). The model of Ischemia-reperfusion (IR1) was established. The model of Ischemia-reperfusion was treated with IPO _ (1) and sham operation (S _ (1)). The rats in group B were perfused with normal saline for 5 days. The model of Ischemia-reperfusion (IR2P) was established. The model of IPO _ 2 and sham operation was established after ischemia-reperfusion. The level of IL-18 in serum SCrBUNNGALL was observed under light microscope under HE staining for observation of renal tissue injury in paraffin-embedded sections of ischemia-reperfusion paraffin-embedded sections after 48 hours of last recovery of renal blood supply. Results compared with IPO1 group, IR1 group, There was no significant difference between IR1 group and IR2 group at each time point. NGALL IL-18 in IR2 group was higher than that in IR1 group at 48 h, and the renal tubular injury score in IR2 group was higher than that in IPO2 group at 24 h after BUN was increased at 12 h. All the indexes in IR2 group were higher than those in IR1 group at 48 h and that in 0.05).IPO1 group was higher than that in IPO2 group. At 6 h, the NGAL of IPO group was significantly higher than that of IPO1 group at 12 h and 12 h, except NGAL at 24 h, which was higher than that in IPO1 group at 48 h, and the degree of renal tubular injury was higher than that in IPO1 group, P 0.05).IR1 group and IPO2 group. At 6 h, the level of NGAL in IPO2 group was significantly higher than that in IR1 group at 12 h except SCr. SCr was still significantly higher than that in IR1 group at 48 h. Conclusion the renal function injury can be alleviated by preventive use of TMZ before IRI in the kidney of rats.
【作者單位】： 中山大學(xué)附屬第五醫(yī)院;
【基金】：廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金資助項(xiàng)目(WSTJJ20101210640102196912170323)
【分類號(hào)】：R965

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本文編號(hào)：1661765