本文選題：右美托咪定　切入點：大鼠　出處：《西安交通大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年05期 　論文類型：期刊論文

【摘要】：目的研究右美托咪定(DEX)對大鼠腸系膜動脈腫瘤壞死因子-α(TNF-α)、白介素-1β(IL-1β)及凋亡相關(guān)因子Caspase-3表達(dá)e笞饔謾７椒ㄈPF級SD雄性大鼠,脫臼處死后,在體視顯微鏡下分離取出腸系膜動脈。建立脂多糖(LPS)誘導(dǎo)的血管損傷實驗?zāi)Ｐ?并根據(jù)隨機化原則將損傷血管分為DEX組和對照組。DEX組根據(jù)所給予DEX的濃度不同分為10~(-8)、10~(-7)、10~(-6) mol/L 3個亞組。分離提取各組動脈組織中RNA及總蛋白后,RT-PCR方法檢測TNF-α、IL-1β及CaSR等因子的mRNA水平;Western blot檢測TNF-α、CaSR、AMPK及Caspase-3等蛋白的表達(dá)變化。結(jié)果 10~(-8)、10~(-7)、10~(-6) mol/L DEX組中LPS誘導(dǎo)的血管炎性反應(yīng)均明顯降低,TNF-α的mRNA和蛋白表達(dá)及IL-1β的mRNA表達(dá)減弱。DEX組血管條的Caspase-3蛋白表達(dá)較LPS組顯著降低。DEX對LPS誘導(dǎo)的血管AMPK蛋白表達(dá)和CaSR的mRNA表達(dá)沒有明顯作用。結(jié)論 DEX明顯降低與炎癥相關(guān)蛋白的表達(dá),提示DEX有一定的抗炎癥作用。
[Abstract]:Objective to study the effect of dexmetomidine (DEX) on the expression of tumor necrosis factor- 偽 (TNF- 偽) and apoptosis related factor (Caspase-3) in mesenteric artery of rats. Male SD rats of grade PF were killed by dislocations. Mesenteric arteries were isolated and removed under stereoscopic microscope. An experimental model of vascular injury induced by lipopolysaccharide (LPS) was established. According to the principle of randomization, the injured vessels were divided into three groups: DEX group and control group. DEX group was divided into three subgroups according to the concentration of DEX. RNA and total protein were isolated and extracted from the arterial tissues of each group. RT-PCR was used to detect TNF- 偽 IL-1 尾 and CaSR. The expression of TNF- 偽 -CaSR-AMPK and Caspase-3 were detected by Western blot. Results in the mol/L DEX group, the expression of mRNA and protein and the mRNA expression of IL-1 尾 were significantly decreased in the vascular strips of the mol/L DEX group. Compared with LPS group, DEX had no significant effect on the expression of AMPK protein and mRNA expression of CaSR induced by LPS. Conclusion DEX can significantly decrease the expression of inflammatory related protein. The results suggest that DEX has some anti-inflammatory effect.
【作者單位】： 西安交通大學(xué)醫(yī)學(xué)部環(huán)境與基因相關(guān)疾病教育部重點實驗室;西安交通大學(xué)第一附屬醫(yī)院轉(zhuǎn)化醫(yī)學(xué)中心;西安交通大學(xué)第一附屬醫(yī)院麻醉手術(shù)部;
【分類號】：R965

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