本文選題：血小板　切入點：富血小板血漿　出處：《中國輸血雜志》2015年11期 　論文類型：期刊論文

【摘要】：目的探討不同血小板制劑對細胞體外增殖活力的影響,并對其作用機制進行初步研究。方法制備不同血小板制劑:新鮮機采血小板、冰凍機采血小板、富血小板凝膠上清、乏血小板血漿等,以1640培養(yǎng)基為對照,與MM.1S細胞(多發(fā)性骨髓瘤細胞)在相同的培養(yǎng)條件下進行共培養(yǎng),用流式細胞儀對培養(yǎng)后的細胞進行細胞計數(shù),觀察細胞的增殖情況。結(jié)果 1)單獨的血小板及乏血小板血漿均對MM.1S細胞有促增殖作用,且促增殖效果與樣品濃度成正相關(guān)。2)新鮮機采血小板對MM.1S細胞促增殖作用在各實驗組中最高,新鮮機采凝膠上清和乏血小板血漿次之;二甲基亞砜對細胞增殖有抑制作用;凝血酶對細胞增殖無明顯影響。3)血小板經(jīng)過冰凍處理后,機采冰凍血小板與冰凍凝膠上清對MM.1S細胞促增殖作用較新鮮機采血小板和凝膠上清減弱。但機采冰凍血小板與冰凍凝膠上清對MM.1S細胞的促增殖作用存在劑量效應(yīng),在稀釋濃度40-80倍左右時,具有相對較強的促增殖影響力。結(jié)論不同血小板制劑對MM.1S細胞的促增殖作用各有不同,此研究將為制備血小板凝膠選擇原輔材料提供實驗依據(jù)和參考。
[Abstract]:Objective to investigate the effect of different platelet preparations on cell proliferation in vitro and its mechanism. Methods different platelets were prepared: fresh platelets, cryopreserved platelets, platelets supernatants, and platelet-rich gel supernatants. The platelet-deficient plasma was co-cultured with MM.1S cells (multiple myeloma cells) in 1640 medium, and the cells were counted by flow cytometry. Results 1) platelets and platelets alone could promote the proliferation of MM.1S cells. The effect of promoting proliferation was positively correlated with the concentration of sample. 2) the effect of fresh platelets on the proliferation of MM.1S cells was the highest, the supernatant of fresh mechanized gel and the plasma of lack of platelets were the second, and dimethyl sulfoxide inhibited the proliferation of MM.1S cells. Thrombin has no significant effect on cell proliferation. The effect of frozen platelets and supernatants on the proliferation of MM.1S cells was weaker than that of fresh platelets and supernatants of gel, but there was a dose effect on the proliferation of MM.1S cells by the supernatant of frozen platelets and supernatants of frozen gels. Conclusion the effects of different platelet preparations on the proliferation of MM.1S cells are different. This study will provide experimental basis and reference for the preparation of raw and auxiliary materials for platelet-gel selection.
【作者單位】： 成都軍區(qū)總醫(yī)院輸血科解放軍成都血站;成都醫(yī)學(xué)院科研實驗中心;
【基金】：2011年四川省衛(wèi)生廳科研課題(血站高效型血小板凝膠的制備及臨床應(yīng)用評價研究編號110474)
【分類號】：R96

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本文編號：1642394