本文選題：腎上腺皮質(zhì)腫瘤　切入點：克隆　出處：《實用醫(yī)學雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的:研究阿霉素在體干預(yù)對腎上腺皮質(zhì)癌系SW-13克隆特性的影響。方法:通過建立阿霉素干預(yù)裸鼠連續(xù)傳代SW-13細胞系皮下移植瘤模型,將空白組及實驗組細胞進行單細胞克隆行成試驗,觀察單個細胞生長曲線,并計算克隆形成率及3種克隆(全克隆、部分克隆和旁克隆)的百分比,并進行Hoechst33342試驗檢測細胞的耐藥性。結(jié)果:SW-13細胞系經(jīng)過阿霉素干預(yù)裸鼠連續(xù)傳代移植瘤模型后,克隆形成率較空白對照組增加(P0.05);全克隆的百分比高于空白對照組(P0.05);經(jīng)Hoechst33342試驗發(fā)現(xiàn)經(jīng)阿霉素干預(yù)腎上腺皮質(zhì)癌細胞熒光減弱。結(jié)論:阿霉素在體干預(yù)提高腎上腺皮質(zhì)癌細胞系SW-13的克隆形成能力及全克隆形成能力,并提高其耐藥性。
[Abstract]:Objective: To study the effect of adriamycin intervention SW-13 clone characteristics of adrenal cortical carcinoma lines in vivo. Methods: through the establishment of adriamycin intervention continuous passaging in nude mice SW-13 cells subcutaneous transplantation tumor model, the blank group and the experimental group were single cell cloned into a single test, observe the cell growth curve and colony forming efficiency was calculated and 3 clone (Quan Kelong, part of cloning and cloning by) the percentage of drug resistance and Hoechst33342 test cells. Results: SW-13 cells after adriamycin intervention after continuous passaging in nude mice xenograft model, clone formation rate than the control group increased (P0.05); the percentage of total clones higher than the control group (P0.05); the the Hoechst33342 trial found that after adriamycin treatment of adrenal cortical carcinoma cells. Conclusion: the fluorescence decrease of adriamycin in body intervention to improve the adrenocortical carcinoma cell line cloned SW-13 formation Force and total cloning ability and increase its resistance.

【作者單位】： 浙江中醫(yī)藥大學附屬第二醫(yī)院;廣西南溪山醫(yī)院;廣西醫(yī)科大學第一附屬醫(yī)院內(nèi)分泌科;廣西醫(yī)科大學基礎(chǔ)醫(yī)學院病生教研室;
【基金】：國家自然科學基金項目(編號:81060220) 廣西教育廳項目(編號:12MS061) 浙江中醫(yī)藥大學校級課題(編號:2014ZY17)
【分類號】：R96

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