本文選題：Luminex液相芯片　切入點(diǎn)：NNK　出處：《環(huán)境與健康雜志》2015年11期 　論文類型：期刊論文

【摘要】：目的了解4-(甲基亞硝胺基)-1-(3-吡啶)-1-丁酮(NNK)和2-氨基-9H-吡啶并[2,3-b]吲哚(AαC)的體外免疫毒性特征。方法分別以0、12.5、25、50、100、150和200μg/ml NNK染毒小鼠淋巴瘤細(xì)胞(EL-4細(xì)胞)24 h,以0、2.5、5、7.5、10、12.5和15μg/ml AαC染毒小鼠巨噬細(xì)胞(Ana-1細(xì)胞)24 h。采用CCK-8法檢測細(xì)胞存活率,以Luminex液相芯片技術(shù)檢測細(xì)胞上清液中與免疫相關(guān)的20種細(xì)胞因子分泌量,以BCA蛋白檢測方法校正細(xì)胞因子的結(jié)果。結(jié)果 NNK染毒EL-4細(xì)胞后,白介素(IL)-10、IL-17、IL-1β和腫瘤壞死因子-α(TNF-α)含量隨染毒劑量的增加而升高,粒細(xì)胞-巨噬細(xì)胞集落刺激因子(GM-CSF)含量隨染毒劑量的增加而降低,且均存在劑量-效應(yīng)關(guān)系;堿性成纖維細(xì)胞生長因子(FGF-basic)、γ-干擾素(IFN-γ)、IL-1α、IL-2、IL-4、IL-5、IL-6、IL-12、IL-13、干擾素誘導(dǎo)蛋白-10(IP-10)、角質(zhì)細(xì)胞誘導(dǎo)因子(KC)、單核細(xì)胞趨化蛋白-1(MCP-1)、γ-干擾素誘導(dǎo)性單核因子趨化因子(MIG)、巨噬細(xì)胞炎癥蛋白-1α(MIP-1α)和血管內(nèi)皮生長因子(VEGF)含量無明顯變化。AαC染毒Ana-1細(xì)胞后,IL-5、MCP-1、MIP-1α、TNF-α和VEGF含量隨染毒劑量的增加而降低,其余15種細(xì)胞因子無明顯變化。結(jié)論 NNK和AαC可以產(chǎn)生體外免疫毒性。
[Abstract]:Objective to investigate the characteristics of in vitro immunotoxicity of 4-( methylnitrosamine-3-pyridine-3-pyridine-1-butanone) and 2-amino-9H-pyridyl [2Amino-9H-pyridine] indole A 偽 C. Methods mice lymphoma cell line EL-4 cells were exposed to 0.12.5rml NNK for 24 h and 2.57.51010g / ml to 12.5 and 15 渭 g / ml A 偽 -C, respectively. The survival rate of murine macrophages, Ana-1 cells, was determined by CCK-8 assay for 24 h. The levels of immune-related cytokines in supernatant of cells were detected by Luminex liquid chip technique, and the results of cytokines were corrected by BCA protein assay. Results after NNK was exposed to EL-4 cells, The contents of IL-17 尾 and TNF- 偽 increased with the increase of exposure dose, and the content of granulocyte-macrophage colony stimulating factor (GM-CSF) decreased with the increase of dose, and there was a dose-effect relationship between the contents of IL-17 尾 and TNF- 偽. Basic fibroblast growth factor FGF-basicus, IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IFN- 緯 -IL-2-, IL-5, IL-6, IL-6, IL-12, Interferon-inducible protein (-10), keratinocyte inducible factor (KCU), monocyte chemoattractant protein (MCP-1), interferon- 緯 -inducible monokine chemokine (IFN- 緯), macrophage inflammatory protein 1 偽 (MIP-1 偽) and intravascular inflammation protein (MIP-1 偽). There was no significant change in the content of skin growth factor (VEGF). The contents of TNF- 偽 and VEGF decreased with the increase of dose of Ana-1 cells. Conclusion NNK and A 偽 C can produce in vitro immunotoxicity.
【作者單位】： 中國煙草總公司鄭州煙草研究院煙草化學(xué)重點(diǎn)實(shí)驗(yàn)室;軍事醫(yī)學(xué)科學(xué)院放射與輻射醫(yī)學(xué)研究所;上海煙草集團(tuán)北京卷煙廠;
【基金】：國家科技支撐計劃項(xiàng)目(2012BAK01B03) 鄭州煙草研究院院長科技發(fā)展基金項(xiàng)目(322014CA0350) 鄭州煙草研究院科技項(xiàng)目(322013CZ0600)
【分類號】：R994
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本文編號：1619976