本文選題：液質(zhì)聯(lián)用法　切入點(diǎn)：谷氨酸　出處：《中國測(cè)試》2015年02期 　論文類型：期刊論文

【摘要】：建立LC-MS同時(shí)檢測(cè)大鼠腦脊液中谷氨酸和γ-氨基丁酸的方法。采用安捷倫ZORBAX SIL(4.6 mm×250 mm×5μm)硅膠正相色譜柱,流動(dòng)相為甲醇-水(0.1%甲酸)=50∶50(v/v),梯度洗脫,流速為1 m L/min。電噴霧離子源(eletrospray ionization,ESI)采用正離子化方式,以多重反應(yīng)檢測(cè)(multiple reaction monitoring,MRM)方式進(jìn)行檢測(cè),用于定量分析的離子反應(yīng)分別為m/z 148.1→m/z 84.1(谷氨酸,[M+H]+),m/z 104.1→m/z 87.1(γ-氨基丁酸,[M+H]+)。測(cè)定大鼠腦脊液中谷氨酸在500~2 500μg/L和γ-氨基丁酸在50~250μg/L質(zhì)量濃度范圍內(nèi),濃度與峰面積呈良好的線性關(guān)系。該方法簡便、準(zhǔn)確、靈敏度高,專屬性強(qiáng),重復(fù)性好,適用于腦脊液中谷氨酸和γ-氨基丁酸的同時(shí)測(cè)定,可為氨基酸類神經(jīng)遞質(zhì)提供有效的檢測(cè)手段。
[Abstract]:A LC-MS method for simultaneous determination of glutamic acid and 緯 -aminobutyric acid in cerebrospinal fluid of rats was established by using ZORBAX SIL(4.6 mm 脳 250mm 脳 5 渭 m silica gel positive phase chromatographic column. The mobile phase consisted of methanol-water 0.1% formic acid 50: 50 v / v 路v / v, gradient elution. The flow rate was 1 mL 路min. The electrospray ion source eletrospray ionizationn (ESI) was detected by positive ionization and multiple reaction monitoring by multiple reaction. The ion reaction used for quantitative analysis was m / z 148.1 respectively. 鈫扢 / z 84.1. 鈫扢 / z 87.1 (緯 -aminobutyric acid, [M H]). The linear relationship between the concentration of glutamic acid and peak area in the range of 500 ~ 2 500 渭 g / L of 緯 -aminobutyric acid and 50 ~ 250 渭 g / L of 緯 -aminobutyric acid in cerebrospinal fluid of rats is simple, accurate, sensitive and specific. It is suitable for simultaneous determination of glutamic acid and 緯 -aminobutyric acid in cerebrospinal fluid and can provide an effective method for the determination of amino acid neurotransmitters.
【作者單位】： 成都醫(yī)學(xué)院藥學(xué)院;
【基金】：成都醫(yī)學(xué)院藥學(xué)重點(diǎn)學(xué)科建設(shè)項(xiàng)目(CYXK2012006)
【分類號(hào)】：O657.63;R927.2

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