本文選題：高效液相色譜法　切入點：頭孢克洛　出處：《中國抗生素雜志》2015年08期 　論文類型：期刊論文

【摘要】：目的建立頭孢克洛原料中雜質C(D-頭孢克洛)的HPLC測定方法。方法采用C18(4.6mm×250mm,5μm)色譜柱;流動相為0.78%磷酸二氫鈉溶液(取磷酸二氫鈉7.8g,加水溶解并稀釋至1000m L,用磷酸調p H值至4.0)-乙腈(90:10);流速1.0m L/min;檢測波長254nm;柱溫35℃。結果雜質C在1.22~2.85μg范圍內線性關系良好(r=0.9995,n=5),平均回收率為99.89%,RSD為0.59%(n=9),定量限為2.038ng。結論該方法簡便、準確、重現(xiàn)性好,可用于頭孢克洛原料中雜質C的檢測。
[Abstract]:Objective to establish a HPLC method for the determination of cefloxacin impurity in cefaclor. Methods the chromatographic column of C18C 4.6mm 脳 250mm ~ 5 渭 m was used. The mobile phase consisted of 0.78% sodium dihydrogen phosphate solution (sodium dihydrogen phosphate 7.8 g, dissolved in water and diluted to 1000 mL), adjusted pH value with phosphoric acid to 4.0% -acetonitrile 90: 10 min, flow rate 1.0 mL / min, detection wavelength 254 nm, column temperature 35 鈩，

本文編號：1605062