本文選題：高效液相色譜法　切入點(diǎn)：注射用達(dá)托霉素　出處：《中國藥師》2016年12期 　論文類型：期刊論文

【摘要】：目的:對(duì)注射用達(dá)托霉素有關(guān)物質(zhì)測(cè)定方法進(jìn)行優(yōu)化。方法:采用IB-SIL C-8(250 mm×4.6 mm,5μm)色譜柱;以0.039 mol·L~(-1)磷酸二氫銨(用1 mol·L~(-1)磷酸溶液調(diào)節(jié)pH至3.25)-乙腈(80∶20)為流動(dòng)相A,以0.039 mol·L~(-1)磷酸二氫銨(用1 mol·L~(-1)磷酸溶液調(diào)節(jié)pH至3.25)-乙腈(50∶50)為流動(dòng)相B;柱溫:20℃;流速:1.5 ml·min~(-1);檢測(cè)波長:214 nm。結(jié)果:注射用達(dá)托霉素在0.39~77.25#g·ml~(-1)線性范圍內(nèi)濃度與峰面積呈良好線性關(guān)系,r=1.000 0,最低檢測(cè)限為2.4 ng,達(dá)托霉素與已知和未知雜質(zhì)分離度良好。結(jié)論:本法可用于注射用達(dá)托霉素的有關(guān)物質(zhì)的測(cè)定。
[Abstract]:Objective: to optimize the method for the determination of daptomycin related substances for injection. Methods: the IB-SIL C-8mm 脳 4.6mm ~ 5 渭 m column was used. The mobile phase A was 0.039 mol 路L ~ (-1) ammonium dihydrogen phosphate (adjusted with 1 mol 路L ~ (-1)) phosphoric acid solution to pH 3.25 ~ (-1) -acetonitrile (80: 20), and 0.039 mol 路L ~ (-1) ammonium dihydrogen phosphate (adjusted to 3. 25% -acetonitrile 50: 50 with 1 mol 路L ~ (-1)) phosphoric acid solution as mobile phase, column temperature was 20 鈩，

本文編號(hào)：1580933