本文選題：紅樹林　切入點：放線菌　出處：《中國抗生素雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的對分離自我國南海紅樹林底泥的鏈霉菌Streptomyces sp.H41-26菌株的發(fā)酵物抗菌活性成分進(jìn)行分離和鑒定。方法對鏈霉菌Streptomyces sp.H41-26菌株的發(fā)酵菌絲體采用95%乙醇提取,醇提取物經(jīng)乙酸乙酯萃取獲得抗菌活性部位,通過ODS開放柱層析分離和反相高效液相制備純化。結(jié)果分離得到4個化合物,經(jīng)NMR和MS等波譜學(xué)方法分別鑒定為:放線菌素D(1)、放線菌素X_2(2)、放線菌素X_β(3)和放線菌素X_a(4)。結(jié)論化合物1~4在0.1mg/mL時,對大腸埃希菌(ATCC8739)、金黃色葡萄球菌(ATCC6538P)、傷寒沙門菌(ATCC6539)、乙型副傷寒沙門菌(50094)和乙型溶血性鏈球菌(ATCC32204)具有明顯的抗菌活性,其中化合物2在0.01mg/mL時,對除大腸埃希菌外的其他4種細(xì)菌抑制效果仍很明顯。
[Abstract]:Objective to isolate and identify the antimicrobial active components of Streptomyces Streptomyces sp.H41-26 from mangrove sediments in the South China Sea. Methods the fermentation mycelia of Streptomyces Streptomyces sp.H41-26 was extracted by 95% ethanol. Ethanol extract was extracted by ethyl acetate to obtain antibacterial active parts. The compounds were separated by ODS column chromatography and purified by reversed phase high performance liquid chromatography. The results of NMR and MS spectra were as follows: actinomycin D _ 1, actinomycin X _ 2, actinomycin X _ 尾 ~ (3) and actinomycin X _ 尾 ~ (3)), and actinomycin X _ 尾 ~ (3)) and actinomycin Xa4 ~ (4). Conclusion compound 1o _ 4 is at 0.1 mg / mL. It has obvious antimicrobial activity against Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538PU, Salmonella typhimurium ATCC65399.Japanese paratyphoid Salmonella (50094) and B hemolytic streptococcus (ATCC32204), in which compound 2 is at 0.01 mg / mL. The inhibitory effect on the other four bacteria except Escherichia coli was still obvious.
【作者單位】： 暨南大學(xué)藥學(xué)院中藥及天然藥物研究所廣東省高校中藥藥效物質(zhì)基礎(chǔ)與創(chuàng)新藥物研究重點實驗室;廣東省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所廣東省植物保護(hù)新技術(shù)重點實驗室;暨南大學(xué)醫(yī)學(xué)院微生物與免疫教研室;
【基金】：國家自然科學(xué)基金面上項目(No.81373320) 廣東省自然基金團(tuán)隊項目(No.2015A030312002) 暨南大學(xué)教育部研究基金(No.21612114)
【分類號】：R915

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