本文關(guān)鍵詞： 兩棲類 皮膚 多肽 鎮(zhèn)痛 抗炎　出處：《南京農(nóng)業(yè)大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：兩棲類的皮膚中含有種類繁多、功效復(fù)雜的生物活性物質(zhì),這些活性物質(zhì)可用于抵御環(huán)境中有害因子的侵犯。兩棲類的主要棲息方式有四種：水棲、半陸棲、陸棲和樹棲,而大多數(shù)雨蛙屬兩棲類是營樹棲生活,且它們一般棲息于水邊的植物叢及林木上。在這樣的生活環(huán)境中它們時(shí)刻都可能遭遇更多源于生物及非生物因素的傷害,如：天敵、蟲類叮咬、微生物感染及刺傷等等,皮膚作為兩棲類生命的第一道防線,在適應(yīng)生存中固然起著非常重要的作用。雨蛙屬綠色或棕色的皮膚不僅使它們更能適應(yīng)叢林生活,而且皮膚中的一些化學(xué)組分對它們的生存也起著至關(guān)重要的作用。在本研究中,我們首次從東北雨蛙皮膚中發(fā)現(xiàn)了兩種新型鎮(zhèn)痛多肽,并命名為analgesin-HJ和analgesin-HJ(I5T),它們分別是由5個(gè)氨基WPVI-NH2和FWPVT-NH2組成的小肽,且分子量分別為659.84 Da和647.78 Da。analgesin-HJs是由171個(gè)氨基酸組成的前體肽序列編碼的,其中含有9個(gè)重復(fù)的analgesin-HJ拷貝和一個(gè)analgesin-HJ(I5T)拷貝。然后,我們對analgesin-HJs可能具有的鎮(zhèn)痛及抗炎癥活性進(jìn)行了研究,我們通過四種重要的鎮(zhèn)痛動物模型對analgesin-HJs的鎮(zhèn)痛作用進(jìn)行研究檢測,這四種鎮(zhèn)痛動物模型包括小鼠醋酸扭體實(shí)驗(yàn)、小鼠福爾馬林實(shí)驗(yàn)、小鼠光輻射熱擺尾實(shí)驗(yàn)及熱板實(shí)驗(yàn),結(jié)果發(fā)現(xiàn)analgesin-HJs具有與陽性對照組嗎啡相當(dāng)?shù)逆?zhèn)痛活性。多肽.analgesin-HJ在角叉菜膠誘導(dǎo)的小鼠足趾腫脹模型中的作用同樣驗(yàn)證了analgesin-HJ抗炎止痛的效果。除此之外,通過用LPS刺激誘導(dǎo)小鼠巨噬細(xì)胞RAW 264.7產(chǎn)生前炎癥因子的實(shí)驗(yàn)中,我們發(fā)現(xiàn)analgesin-HJ具有抑制小鼠巨噬細(xì)胞中前炎癥因子釋放的作用。在對analgesin-HJ鎮(zhèn)痛機(jī)制的初步研究中,我們發(fā)現(xiàn)analgesin-HJ沒有明顯的阿片受體激動劑活性,其鎮(zhèn)痛作用機(jī)制還有待進(jìn)一步深入研究。analgesin-HJs易于合成、運(yùn)輸及儲存,加上它們較強(qiáng)的鎮(zhèn)痛效果,analgesin-HJs可能為未來開發(fā)新型止痛劑提供研究基礎(chǔ)。
[Abstract]:The skin of amphibians contains a wide variety of complex bioactive substances that can be used to protect against harmful factors in the environment. There are four main habitats for amphibians: aquatic, semi-terrestrial, terrestrial and arboreal. Most of the amphibians of the genus Rana are tree-dwelling, and they generally live on waterfront vegetation and trees. In such living environments, they are likely to suffer more damage from biological and abiotic factors, such as natural enemies. Insect bites, microbial infections, stab wounds, and so on. Skin, as the first line of defense for amphibian life, plays a very important role in adapting to survival. The green or brown skin of Rana not only makes it more adaptable to jungle life, Some chemical components in the skin also play an important role in their survival. In this study, we found two new analgesic peptides for the first time from the skin of the Northeastern Rana Rana. They are small peptides composed of five amino WPVI-NH2 and FWPVT-NH2, and their molecular weights are 659.84 Da and 647.78 Da.analgesin-HJs respectively, which are encoded by 171 amino acids. There were nine duplicate copies of analgesin-HJ and one copy of analgesin-HJN I5T. Then we studied the possible analgesic and anti-inflammatory activities of analgesin-HJs. We studied the analgesic effects of analgesin-HJs through four important analgesic animal models. The four animal models of analgesia include acetic acid writhing test, formalin test, light radiation tail test and hot plate test. The results showed that analgesin-HJs had the same analgesic activity as that of morphine in positive control group. The effect of polypeptide. Analgesin-HJ on mice toe swelling induced by carrageenin also confirmed the anti-inflammatory and analgesic effect of analgesin-HJ. In the experiment of inducing mouse macrophage RAW 264.7 to produce proinflammatory factor by LPS stimulation, we found that analgesin-HJ can inhibit the release of proinflammatory factor in mouse macrophage. We found that analgesin-HJ has no obvious opioid receptor agonist activity, and its analgesic mechanism needs to be further studied. The analgesin-HJs are easy to synthesize, transport and store. Combined with their strong analgesic effect, analgesin-HJs may provide a basis for the development of new painkillers in the future.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965;Q516

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本文編號：1529424