本文關(guān)鍵詞： 轉(zhuǎn)化生長(zhǎng)因子β 血管損傷 平滑肌細(xì)胞　出處：《中國(guó)老年學(xué)雜志》2015年08期 　論文類型：期刊論文

【摘要】：目的探討轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)及其受體對(duì)血管損傷后內(nèi)膜增殖的作用及機(jī)制。方法培養(yǎng)SD大鼠正常及損傷頸動(dòng)脈血管平滑肌細(xì)胞,應(yīng)用逆轉(zhuǎn)錄PCR(RT-PCR)及蛋白印跡(Western印跡)方法分別檢測(cè)血管平滑肌細(xì)胞(VSMCs)生物學(xué)標(biāo)記物SM22α,Matrix Gla,Osteopontin;TGF-β1,纖維連結(jié)蛋白(FN)及TGF-β1Ⅰ型和Ⅱ型受體(TGF-β1-RⅠ,TGF-β1-RⅡ)mRNA和蛋白質(zhì)的表達(dá)。結(jié)果 1TGF-β1呈濃度依賴性刺激損傷頸動(dòng)脈VSMCs增殖;但在0.2%血清條件下,TGF-β1濃度依賴性抑制正常頸動(dòng)脈VSMCs增殖;2正常組VSMCs SM22αmRNA表達(dá)水平明顯高于損傷組VSMCs;而Matrix Gla及Osteopontin損傷組VSMCs的表達(dá)水平則顯著升高;3損傷組VSMCs TGF-β1 mRNA和蛋白質(zhì)的表達(dá)水平顯著高于正常組,且FN的合成也較正常組顯著增高;4損傷組TGF-β1-RⅠmRNA和蛋白質(zhì)的表達(dá)與正常組并無(wú)明顯差別;但TGF-β1-RⅡ的表達(dá)水平損傷組卻顯著高于正常組。結(jié)論血管損傷后,VSMCs表型發(fā)生改變,呈現(xiàn)異常增殖,合成和分泌TGF-β1及FN明顯增多;TGF-β1對(duì)損傷后VSMCs有顯著促增殖效應(yīng);同時(shí)血管損傷后VSMCs TGF-β1不同受體亞型的表達(dá)也發(fā)生了變化。
[Abstract]:Objective to investigate the effect and mechanism of transforming growth factor 尾 1 (TGF- 尾 1) and its receptor on intimal proliferation after vascular injury. Methods normal and injured carotid vascular smooth muscle cells were cultured in SD rats. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot (Western blot) were used to detect the expression of TGF- 尾 1 and TGF- 尾 1-R 鈪，

本文編號(hào)：1519358