本文關(guān)鍵詞： 福美司坦 同位素比質(zhì)譜 興奮劑　出處：《中國運(yùn)動醫(yī)學(xué)雜志》2015年06期 　論文類型：期刊論文

【摘要】：目的:建立對福美司坦藥物來源的檢測方法,并進(jìn)行人體代謝的檢測研究。方法:采用酶解、提取、高效液相色譜純化等方法對尿樣進(jìn)行預(yù)處理,應(yīng)用同位素比質(zhì)譜儀測定相關(guān)組分的同位素比(13C/12C,δ值),建立檢測方法。本實驗中招募2名男性健康受試者,每位受試者口服福美司坦后收集尿樣,應(yīng)用所建立的檢測方法對尿樣進(jìn)行分析,探討福美司坦在人體內(nèi)代謝過程中的檢測規(guī)律。結(jié)果:建立的分析方法精密度及重現(xiàn)性RSD均小于1%。受試者尿樣中福美斯坦的δ值在服藥后迅速降低,并在27小時內(nèi)保持在約為-31‰。內(nèi)源性雄性激素代謝物雄酮androsterone和本膽烷醇酮etiocholanolone的δ值無顯著性變化。結(jié)論:在興奮劑檢測中確定以福美司坦藥物原形為目標(biāo)化合物進(jìn)行來源分析,建立了檢測福美斯坦興奮劑的同位素比質(zhì)譜分析方法。
[Abstract]:Objective: to establish a method for the detection of the origin of Fumestan and to study the metabolism of human body. Methods: urine samples were pretreated by enzymolysis, extraction and purification by HPLC. The isotopic ratio of 13C / 12C, 未 value of the related components was determined by means of isotope mass spectrometer, and a method was established. Two healthy male subjects were recruited in this experiment, and each of them took Fumestan orally and collected urine samples. The urine samples were analyzed by the established method. Objective: to study the detection rule of Fumestan during metabolism in human body. Results: the precision and reproducibility of the method were less than 1. The 未 value of Fumestan in urine decreased rapidly after taking medicine. The 未 values of androsterone androsterone and etiocholanolone, the endogenous metabolites of androgen, did not change significantly within 27 hours. Conclusion: the target combination of Fumestan drug in stimulant detection is determined. For source analysis, A mass spectrometry method for the determination of fumestan stimulants was established.
【作者單位】： 國家體育總局反興奮劑中心;北京體育大學(xué);北京中醫(yī)藥大學(xué);
【基金】：國家體育總局重點研究領(lǐng)域攻關(guān)課題(編號:2013B003)
【分類號】：R927;O657.63

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1 胡永洲,劉滔,姚愛平,楊大為;福美司坦的合成方法改進(jìn)[J];浙江大學(xué)學(xué)報(醫(yī)學(xué)版);2001年01期

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本文編號：1506108