本文關鍵詞： IR780 全氟己烷 快速可控 微囊 膠原蛋白　出處：《南京大學》2014年碩士論文　論文類型：學位論文

【摘要】：近年來,高分子聚合物PLGA已經被廣泛地用于抗腫瘤藥載藥系統(tǒng)的研究與應用中。作為一種理想的藥物載體,PLGA具有良好的生物相容性、生物可降解性以及合適的相轉變溫度。但是,常溫下PLGA聚合物鏈之間交聯較為緊密,使所載藥物難以釋放出來。載體中藥物的緩慢釋放使腫瘤細胞周圍藥物濃度往往達不到將其殺滅的閾值,因此導致對腫瘤細胞增值的抑制效果較差。本文設計了一種新型藥物遞送系統(tǒng),該系統(tǒng)可以在近紅外激光觸發(fā)下短時間內釋放出大量所載藥物。該系統(tǒng)是一種新型的載藥PLGA復合微囊,廣譜抗腫瘤藥物阿霉素(doxorubicin,DOX)和近紅外染料IR-780被均勻分散于微囊疏水性的PLGA殼層中,而液態(tài)全氟己烷(PFH)被包裹在微囊核中。當用近紅外激光照射后,由于IR-780的存在,微囊及其周圍溶液溫度快速升高,包裹在微囊中的液態(tài)全氟己烷氣化使微囊體積膨脹甚至引起微囊殼層破裂,微囊殼層中的阿霉素被快速大量地釋放出來。同時,高溫使PLGA鏈之間的交聯變得松散也會促進阿霉素的釋放。在腫瘤細胞懸液中加入該微囊,并定時用近紅外激光照射后,腫瘤細胞的存活率明顯降低,微囊表現出了良好的細胞毒效應。該微囊能夠克服以PLGA為載藥材料的藥物遞送系統(tǒng)中所載藥物釋放緩慢的缺點,是一種有應用潛力的快速可控釋藥載體。膠原蛋白具有良好的生物相容性及低免疫原性,近年來膠原蛋白被廣泛地應用于組織工程、醫(yī)療器械以及載藥系統(tǒng)中。本文研究了從新鮮豬皮中提取和純化Ⅰ型膠原蛋白的方法,并用十二烷基磺酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)和傅里葉變紅外光譜(FTIR)對所提膠原進行了分析鑒定,證明了所制備的膠原蛋白為Ⅰ型。
[Abstract]:In recent years, polymer PLGA has been widely used in the research and application of anti-tumor drug delivery system. As an ideal drug carrier, PLGA has good biocompatibility. Biodegradability and appropriate phase transition temperature. However, the cross-linking of PLGA polymer chains is relatively close at room temperature. The slow release of drugs in the carrier often causes the concentration of drugs around tumor cells to fail to reach the threshold to kill them, As a result, the inhibition of tumor cell proliferation was poor. A novel drug delivery system was designed in this paper. The system can release a large amount of drugs under the trigger of near infrared laser in a short time. The system is a new drug loaded PLGA composite microcapsule. The broad-spectrum antitumor drug doxorubicindox) and the near infrared dye IR-780 were uniformly dispersed in the hydrophobic PLGA shell of the microcapsules, while the liquid perfluorohexane was encapsulated in the microcapsule nucleus. When irradiated with near infrared laser, the presence of IR-780 was observed. The liquid perfluorohexane encapsulated in the microcapsules expands in volume and even causes the microcapsule shell to rupture, and the adriamycin in the microcapsule shell is released rapidly and in large quantities. The release of adriamycin was also promoted by the loosening of crosslinking between PLGA chains by high temperature. The survival rate of tumor cells was significantly decreased after the microcapsules were added to the suspension of tumor cells and irradiated with NIR laser at regular intervals. The microcapsules exhibit good cytotoxic effects. The microcapsules can overcome the disadvantages of slow release of the drugs contained in the drug delivery system using PLGA as the drug carrier. Collagen has good biocompatibility and low immunogenicity. In recent years, collagen has been widely used in tissue engineering. In medical instruments and drug delivery systems, a method for extracting and purifying collagen type I from fresh pig skin was studied. The extracted collagen was identified by SDS-PAGE and Fourier transform infrared spectroscopy (FTIR). It was proved that the prepared collagen was type 鈪，

本文編號：1504923