本文關(guān)鍵詞： 慢性不可預(yù)見(jiàn)性應(yīng)激 谷氨酸轉(zhuǎn)運(yùn)體 氟西汀 前額葉 免疫組織化學(xué)法 蛋白印跡分析 抑郁　出處：《中國(guó)藥理學(xué)通報(bào)》2015年02期 　論文類(lèi)型：期刊論文

【摘要】：目的研究氟西汀對(duì)慢性應(yīng)激大鼠前額葉谷氨酸轉(zhuǎn)運(yùn)體1(GLT-1)的影響,進(jìn)一步探討氟西汀抗抑郁作用可能的分子機(jī)制。方法正常SD大鼠60只,隨機(jī)分為對(duì)照組、慢性不可預(yù)見(jiàn)性應(yīng)激(CUS)組和氟西汀組。對(duì)CUS組和氟西汀組大鼠進(jìn)行CUS應(yīng)激后,氟西汀組給予氟西汀治療,對(duì)照組和CUS組給予生理鹽水。實(shí)驗(yàn)結(jié)束后進(jìn)行糖水偏好和曠場(chǎng)行為測(cè)試,并使用免疫組織化學(xué)法和蛋白印跡分析檢測(cè)大鼠前額葉GLT-1的表達(dá)水平。結(jié)果 (1)行為學(xué)測(cè)試結(jié)果顯示,CUS組大鼠糖水偏好、總行程、平均移動(dòng)速度及直立次數(shù)均低于對(duì)照組(P0.01);氟西汀組上述指標(biāo)均高于CUS組(P0.01)。(2)免疫組織化學(xué)法分析顯示,CUS組與對(duì)照組比較,大鼠前額葉GLT-1表達(dá)下降(P0.01);經(jīng)氟西汀治療后,大鼠前額葉GLT-1表達(dá)比CUS組明顯升高(P0.01)。(3)蛋白印跡分析顯示,CUS組與對(duì)照組比較,大鼠前額葉GLT-1表達(dá)下降(P0.01);經(jīng)氟西汀治療后,大鼠前額葉GLT-1表達(dá)比CUS明顯升高(P0.01)。結(jié)論慢性應(yīng)激下調(diào)大鼠前額葉GLT-1表達(dá)水平,而氟西汀上調(diào)GLT-1表達(dá)水平,GLT-1表達(dá)增加可能是氟西汀抗抑郁作用的分子機(jī)制之一。
[Abstract]:Objective to study the effect of fluoxetine on the prefrontal glutamate transporter (GLT-1) in rats under chronic stress and to explore the possible molecular mechanism of antidepressant effect of fluoxetine. The rats in CUS group and fluoxetine group were treated with fluoxetine after CUS stress. The control group and CUS group were given normal saline. After the experiment, sugar water preference and open-field behavior were tested. Immunohistochemical method and Western blot analysis were used to detect the expression of GLT-1 in prefrontal lobe of rats. The average moving speed and the number of upright movement were lower than that of the control group (P 0.01). The above indexes in fluoxetine group were higher than those in CUS group (P 0.01). The immunohistochemical analysis showed that the CUS group was compared with the control group. The expression of GLT-1 in prefrontal lobe of rats was decreased (P 0.01). After fluoxetine treatment, the expression of GLT-1 in the prefrontal lobe of rats was significantly higher than that in the CUS group. Western blot analysis showed that the expression of GLT-1 in the prefrontal lobe of rats was higher than that in the control group. The expression of GLT-1 in prefrontal lobe of rats was decreased (P 0.01). The expression of GLT-1 in prefrontal lobe of rats was significantly higher than that of CUS after fluoxetine treatment. Conclusion chronic stress can down-regulate the expression of GLT-1 in prefrontal lobe of rats. But fluoxetine upregulated GLT-1 expression and GLT-1 expression may be one of the molecular mechanisms of fluoxetine antidepressant effect.
【作者單位】： 武漢大學(xué)人民醫(yī)院精神衛(wèi)生中心;湖北大學(xué)教育學(xué)院心理學(xué)系;
【基金】：國(guó)家“十二五計(jì)劃”項(xiàng)目課題(No 2012BAI01B05) 國(guó)家自然科學(xué)基金資助項(xiàng)目(No 30971040,81271496)
【分類(lèi)號(hào)】：R965
【正文快照】： 0.01)。(3)蛋白印跡分析顯示,CUS組與對(duì)照組比較,大鼠前額葉GLT-1表達(dá)下降(P0.01);經(jīng)氟西汀治療后,大鼠前額葉GLT-1表達(dá)比CUS明顯升高(P0.01)。結(jié)論慢性應(yīng)激下調(diào)大鼠前額葉GLT-1表達(dá)水平,而氟西汀上調(diào)GLT-1表達(dá)水平,GLT-1表達(dá)增加可能是氟西汀抗抑郁作用的分子機(jī)制之一。當(dāng)

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