發(fā)布時(shí)間：2018-01-18 14:33

  本文關(guān)鍵詞：免疫抑制劑對(duì)酵母聚糖誘導(dǎo)巨噬細(xì)胞Dectin-1、Toll樣受體2及腫瘤壞死因子-α表達(dá)的影響　出處：《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2016年21期 　論文類型：期刊論文

  更多相關(guān)文章： 霉酚酸酯 環(huán)孢素A 酵母聚糖 模式識(shí)別受體 細(xì)胞因子

【摘要】：目的研究霉酚酸酯和環(huán)孢素A對(duì)酵母聚糖(Zymosan A)誘導(dǎo)小鼠RAW264.7巨噬細(xì)胞模式識(shí)別受體Dectin-1、Toll樣受體2(TLR2)表達(dá)及細(xì)胞因子腫瘤壞死因子-α(TNF-α)釋放的影響。方法體外培養(yǎng)RAW264.7巨噬細(xì)胞,分別給予不同濃度的霉酚酸酯、環(huán)孢素A預(yù)處理細(xì)胞24 h,再利用100μg/ml Zymosan A單獨(dú)刺激細(xì)胞,逆轉(zhuǎn)錄聚合酶鏈反應(yīng)和流式細(xì)胞術(shù)檢測(cè)細(xì)胞Dectin-1、TLR2 m RNA和蛋白水平的變化,酶聯(lián)免疫吸附試驗(yàn)檢測(cè)上清液中TNF-α濃度的變化。結(jié)果 Zymosan A單獨(dú)作用巨噬細(xì)胞Dectin-1、TLR2 m RNA和蛋白水平明顯上調(diào),TNF-α濃度升高(P0.05)。Zymosan A作用于霉酚酸酯或環(huán)孢素A預(yù)處理24 h的巨噬細(xì)胞Dectin-1、TLR2 m RNA和蛋白水平較Zymosan A單獨(dú)作用組明顯下調(diào),TNF-α分泌量減少(P0.05)。結(jié)論霉酚酸酯和環(huán)孢素A抑制巨噬細(xì)胞Dectin-1和TLR2的轉(zhuǎn)錄和翻譯,并下調(diào)TNF-α的釋放,降低機(jī)體對(duì)真菌病原體的清除能力,這可能是應(yīng)用霉酚酸酯和環(huán)孢素A引起難控性真菌感染的機(jī)制之一。
[Abstract]:Objective to study the effect of mycophenolate mofetil and cyclosporine A on the pattern recognition receptor (Dectin-1) of RAW264.7 macrophage induced by Zymosan A. The expression of Toll like receptor 2 (TLR2) and the release of cytokine tumor necrosis factor- 偽 (TNF- 偽) were investigated. Methods RAW264.7 macrophages were cultured in vitro. The cells were pretreated with different concentrations of mycophenolate mofetil and cyclosporine A for 24 h and stimulated with 100 渭 g / ml Zymosan A. Reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry (FCM) were used to detect the changes of TLR2 m RNA and protein levels in the cells. The concentration of TNF- 偽 in supernatant was detected by enzyme-linked immunosorbent assay (Elisa). Results Zymosan A alone acted on macrophage Dectin-1. The levels of TLR2 m RNA and protein were up-regulated. When the concentration of TNF- 偽 was increased, the macrophage Dectin-1 was pretreated with mycophenolate mofetil or cyclosporine for 24 h. The levels of TLR2 m RNA and protein were significantly down-regulated than those of Zymosan A alone. Conclusion mycophenolate mofetil and cyclosporine A inhibit the transcription and translation of Dectin-1 and TLR2 in macrophages, and down-regulate the release of TNF- 偽. It may be one of the mechanisms that mycophenolate mofetil and cyclosporine A can induce refractory fungal infection by reducing the ability of mycophenolate mofetil and cyclosporine A.
【作者單位】： 安徽醫(yī)科大學(xué);空軍總醫(yī)院呼吸內(nèi)科;
【分類號(hào)】：R96
【正文快照】： 免疫抑制劑被廣泛應(yīng)用于自身免疫病及移植抗宿主病的預(yù)防和治療,目的在于控制免疫反應(yīng)的加劇[1]。然而長(zhǎng)期接受大劑量免疫抑制劑治療,機(jī)體免疫功能遭到嚴(yán)重破壞,對(duì)外界病原菌的抵抗力減弱,極易發(fā)生難以控制的感染性疾病,尤其是侵襲性真菌感染,嚴(yán)重威脅免疫抑制患者的生存。巨

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