本文關(guān)鍵詞：紅樹林內(nèi)生真菌Aspergillus sp.085035次級代謝產(chǎn)物研究　出處：《中藥材》2017年02期 　論文類型：期刊論文

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【摘要】：目的:研究紅樹林內(nèi)生真菌Aspergillus sp.085035的次級代謝產(chǎn)物。方法:采用柱色譜和半制備高效液相色譜技術(shù)對Aspergillus sp.085035的次級代謝產(chǎn)物進行分離純化,并利用波譜數(shù)據(jù)和X-射線單晶衍射等方法鑒定化合物結(jié)構(gòu);采用MTT法對所得化合物進行細胞毒活性檢測。結(jié)果:從紅樹林內(nèi)生真菌Aspergillus sp.085035的乙酸乙酯粗提物中分離得到9個化合物,分別鑒定為:13R-fumigaclavine C(1)、8'-O-methylasterric acid(2)、1,2-secotrypacidin(3)、rhizoctonic acid(4)、chaetominine(5)、helvolic acid(6)、trypacidin(7)、emodin-8-methylether(8)和fumitremorgin C(9)。結(jié)論:化合物1為新天然產(chǎn)物,化合物1、8為首次從曲霉屬中分離得到,所有化合物均為首次從該真菌中分離得到,化合物7對MDA-MB-435細胞株具有細胞毒活性。
[Abstract]:Objective: to study the secondary metabolites of endophytic fungus Aspergillus sp.085035 in mangrove forests. The secondary metabolites of Aspergillus sp.085035 were separated and purified by column chromatography and semi-preparative high performance liquid chromatography. The structures of the compounds were identified by means of spectral data and X-ray single crystal diffraction. The cytotoxic activity of the compounds was determined by MTT method. Results: the endophytic fungus Aspergillus from mangrove forests was used to detect the cytotoxicity of the compounds. Nine compounds were isolated from the ethyl acetate extract of sp.085035. They were identified as: 13R-fumigaclavine, Candellavine, 8- O-methylasterric acidine, respectively. 2-secoTrypacidinine 3 Rhizoctonic acidinine 4). Helvolic acidin7). Conclusion: compound 1 is a new natural product and compound 1 is a new natural product. 8 was isolated from Aspergillus for the first time and all compounds were isolated from the fungus for the first time. Compound 7 has cytotoxic activity to MDA-MB-435 cell line.
【作者單位】： 中山大學海洋科學學院;中山大學化學化工學院;武漢大學藥學院;南海生物資源開發(fā)與利用協(xié)同創(chuàng)新中心;
【基金】：國家自然科學基金(21272268) 海洋生物天然產(chǎn)物化合物庫基金(4109041)
【分類號】：R915
【正文快照】： 曲霉屬Aspergillus sp.屬于散囊菌目發(fā)菌科的腐生菌,在自然界分布廣泛,是世界上最常見的真菌之一。目前,人們對曲霉屬真菌進行了非常全面的研究,從曲霉菌的次級代謝產(chǎn)物中發(fā)現(xiàn)了許多具有特殊結(jié)構(gòu)、良好生物活性的化合物,如fumigaclavineC[1],aniquinazolines A-D[2],fumitremo

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