本文關(guān)鍵詞：人單核細(xì)胞THP-1源性泡沫細(xì)胞內(nèi)雷帕霉素含量測(cè)定的HPLC法的建立及應(yīng)用　出處：《中國(guó)藥房》2017年01期 　論文類型：期刊論文

  更多相關(guān)文章： 高效液相色譜法 雷帕霉素 含量測(cè)定 靶向藥物 泡沫細(xì)胞

【摘要】：目的:建立人單核細(xì)胞THP-1源性泡沫細(xì)胞內(nèi)雷帕霉素(RAPA)含量的測(cè)定方法,研究RAPA靶向制劑(RAPA-NP-Apt)對(duì)泡沫細(xì)胞的靶向作用。方法:以氧化型低密度脂蛋白誘導(dǎo)THP-1細(xì)胞建立泡沫細(xì)胞模型,取200 ng/m L的RAPA和200、400、800ng/m L的RAPA-NP-Apt與泡沫細(xì)胞共同孵育60 min。采用高效液相色譜法(HPLC)測(cè)定細(xì)胞內(nèi)RAPA含量,色譜柱為Diamonsil C18,流動(dòng)相為乙腈-水(90∶10,V/V),流速為1.0 m L/min,柱溫為40℃,檢測(cè)波長(zhǎng)為278 nm,進(jìn)樣量為20μL。結(jié)果:RAPA檢測(cè)質(zhì)量濃度線性范圍為50~6 400 ng/m L(r=0.999 96),平均回收率為98.72%(RSD=0.62%,n=3),日內(nèi)、日間RSD均不大于6.15%(n=6),穩(wěn)定性試驗(yàn)的RSD2%(n=6),重復(fù)性試驗(yàn)的RSD=1.64%(n=6)。RAPA和低、中、高質(zhì)量濃度的RAPA-NP-Apt與泡沫細(xì)胞共孵育后RAPA的含量分別為12、43、98、140 ng/10~6個(gè)細(xì)胞。結(jié)論:本方法操作簡(jiǎn)單,穩(wěn)定性和重復(fù)性好,可用于泡沫細(xì)胞內(nèi)RAPA的含量測(cè)定。RAPA-NP-Apt可提高RAPA對(duì)泡沫細(xì)胞的靶向作用。
[Abstract]:Objective: to establish a method for the determination of rapamycin A in human monocyte THP-1 derived foam cells. Objective: to study the targeting effect of RAPA targeting preparation RAPA-NP-Apt on foam cells. Methods: THP-1 cells were induced by oxidized low density lipoprotein (OLDL) to establish foam cell model. The RAPA and 200,400 of 200 ng/m L were selected. #number0# ng / mL RAPA-NP-Apt was incubated with foam cells for 60 min. The content of RAPA was measured by high performance liquid chromatography (HPLC). The chromatographic column was Diamonsil C18, the mobile phase was acetonitrile-water (90: 10V / V), the flow rate was 1.0 mL / min, and the column temperature was 40 鈩，

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