本文關(guān)鍵詞：化瘀通絡(luò)中藥對肝纖維化大鼠的治療作用及其對血小板衍生生長因子（PDGF）及Ras信號轉(zhuǎn)導(dǎo)通路的影響，由筆耕文化傳播整理發(fā)布。

        目的：肝纖維化（Hepatic fibrosis，HF）是各種慢性肝病發(fā)展為肝硬化的一個動態(tài)過程及共同病理途徑，是肝臟纖維結(jié)締組織過度沉積的結(jié)果。肝纖維化是機體對慢性損傷的主動性修復(fù)反應(yīng),以肝臟細(xì)胞外基質(zhì)（Extra-cellular matrix，ECM）的增生與沉積為特征,形態(tài)上表現(xiàn)為肝竇毛細(xì)血管化與肝小葉內(nèi)纖維化，功能上可引起肝功能減退、門靜脈高壓等，甚至發(fā)展成為肝癌，嚴(yán)重影響患者的生命健康。因此，及時治療阻斷肝纖維化的進(jìn)一步發(fā)展意義重大。很多細(xì)胞因子參與了肝纖維化的發(fā)生發(fā)展，血小板衍生生長因子（Platelet derivative growth factor，PDGF）是已知的最強的促肝星狀細(xì)胞（Hepatic stellate cell，HSC）增殖分化的細(xì)胞因子，它能夠強烈刺激HSC的增殖、遷移,轉(zhuǎn)化為肌成纖維細(xì)胞,促使膠原產(chǎn)生、沉積,在肝纖維化的發(fā)生發(fā)展中意義重大。國內(nèi)外學(xué)者對肝纖維化的發(fā)病機制進(jìn)行了大量研究，但臨床上阻斷肝纖維化仍是一個難題，近年來開展的中醫(yī)藥抗肝纖維化的臨床和基礎(chǔ)研究顯示了一定的優(yōu)勢和較大的潛力�；鐾ńj(luò)中藥鱉甲煎丸出自張仲景《金匾要略》，具有化瘀通絡(luò)、軟堅散結(jié)之功效，攻補兼施，作用全面。大量實驗證實，鱉甲煎丸應(yīng)對肝纖維化的復(fù)雜病機可通過多途徑、多靶點的發(fā)揮中醫(yī)藥抗肝纖維化的作用。本研究通過對SD大鼠采用皮下注射四氯化碳（CCL4）的方法建立肝纖維化大鼠模型，應(yīng)用化瘀通絡(luò)中成藥鱉甲煎丸對該模型進(jìn)行干預(yù)，通過觀察實驗動物的一般情況，檢測血清層粘連蛋白（LN）、Ⅳ型膠原（Col-Ⅳ），血清谷丙轉(zhuǎn)氨酶（ALT）、谷草轉(zhuǎn)氨酶（AST）、堿性磷酸酶（ALP）、血清白蛋白（ALB）的含量，觀察肝臟病理學(xué)形態(tài)，檢測肝組織PDGF水平，肝組織Ras、細(xì)胞外信號調(diào)節(jié)激酶（Extracellular signal regulated kinase，ERK1）水平，探討化瘀通絡(luò)中藥對肝纖維化的防治作用及其可能作用機制，為臨床上治療以“氣陰兩虛、瘀血阻絡(luò)”為基本病因病機的“癥積”提供實驗依據(jù)。方法：清潔級健康雄性SD大鼠48只，體重280-320g,適應(yīng)性飼養(yǎng)一周后按體重隨機分為正常對照組（Control）、模型組（Model）、西藥組（Thewestern medicine group，簡稱XY）、化瘀通絡(luò)中藥低劑量組（Low dosetreated）簡稱ZY（L）、中劑量組（Middle dose treated）簡稱ZY（M）、高劑量組（High dose treated）簡稱ZY（G）。除正常組外，模型組、中藥各組、西藥組均于實驗第1天每只大鼠皮下注射40%CCl4花生油5ml/kg，此后每3天注射3ml/kg，同時從實驗第一天起，中藥各組分別給予化瘀通絡(luò)中藥低、中、高劑量組大鼠鱉甲煎丸0.55g/（kg.d）、1.1g/（kg.d）、2.2g/（kg.d）灌胃，西藥組給予秋水仙堿０.3mg/kｇ灌胃，每日一次。從實驗第四周末開始，每周末隨機處死模型組大鼠，光鏡下觀察肝臟病理學(xué)改變，實驗第6周造模成功。造模成功后給予中藥各組大鼠及西藥組大鼠繼續(xù)灌胃5周，每日一次。從實驗第一天開始，模型組大鼠給予蒸餾水灌胃，共11周。整個實驗中，正常組大鼠皮下注射等量花生油，并給予蒸餾水灌胃,每日1次，共6周,6周停止皮下注射花生油，繼續(xù)給予蒸餾水灌胃5周。于實驗第11周末，禁食12小時，水合氯醛麻醉，股動脈放血，分離血清，測定血清ALT、AST、ALP、ALB水平；放射免疫法檢測血清LN、Col-Ⅳ水平；摘取大鼠肝臟，留取肝組織，置于4%多聚甲醛固定液中，應(yīng)用HE染色及Masson染色觀察各組大鼠的肝臟病理形態(tài)學(xué)變化；留取肝組織于液氮速凍后置于-800C低溫冰箱內(nèi)保存，反轉(zhuǎn)錄聚合酶聯(lián)反應(yīng)（reverse transcription-polymerase chainreaction RT-PCR）法檢測肝組織中PDGF含量、Ras蛋白水平；免疫組化方法檢測肝組織中ERK1的表達(dá)。結(jié)果：1各組大鼠的一般情況在實驗過程中，正常組除外，其余各組均有死亡情況:模型組死亡3只，中藥高劑量組死亡1只，中藥低、中劑量組各死亡2只，西藥組死亡3只。正常組大鼠體重呈進(jìn)行性增長，飲食正常，反應(yīng)敏捷，眼光有神，毛發(fā)有光澤。其余各組大鼠一般狀況較差，反應(yīng)遲鈍，皮毛松弛，毛色暗淡，潮濕成縷，其中模型組個別大鼠出現(xiàn)雙目失明，且脾氣急躁易怒，易激惹，形體消瘦。2各組大鼠肝臟病理形態(tài)學(xué)改變正常組大鼠肝臟形態(tài)正常，包膜完整，色淡紅，切面光滑整齊。肝小葉結(jié)構(gòu)清晰，肝細(xì)胞多為單核，肝板呈條索狀，肝細(xì)胞圍繞中央靜脈呈放射狀排列。模型組大鼠，肝臟形態(tài)異常，表面毛糙、質(zhì)脆，切面有沙粒感。大鼠肝細(xì)胞腫脹變大，肝細(xì)胞廣泛壞死，肝臟匯管區(qū)擴大及炎細(xì)胞浸潤，肝小葉結(jié)構(gòu)破壞，匯管區(qū)纖維結(jié)締組織增生明顯，將肝小葉包繞成大小不等的肝細(xì)胞團，形成假小葉�；鐾ńj(luò)中藥各劑量組肝小葉結(jié)構(gòu)較清晰，肝竇輕度擴張，肝細(xì)胞中度壞死，匯管區(qū)纖維結(jié)締組織中度增生，假小葉形成明顯較少。西藥組肝臟病理變化介于模型組與中藥組之間。提示，化瘀通絡(luò)中藥可改善肝纖維化大鼠的肝臟纖維化程度，且相對于西藥秋水仙堿效果更明顯。3各組大鼠血清ALT、AST、ALP、ALB水平各組大鼠血清ALT、AST、ALP含量結(jié)果顯示：與正常組相比，模型組、西藥組、中藥低、中劑量組大鼠血清ALT、AST、ALP含量明顯升高（P<0.05）,中藥高劑量組與正常組ALT、AST、ALP含量差異無統(tǒng)計學(xué)意義；與模型組相比，中藥各劑量組、西藥組血清ALT、AST、ALP明顯降低（P<0.05）；與西藥組相比，中藥高劑量組血清ALT、AST、ALP含量明顯減低（P<0.05），中藥低劑量組含量較高（P<0.05），中藥中劑量組與之差異無統(tǒng)計學(xué)意義；中藥各劑量組之間相比，中藥低劑量組血清ALT、AST、ALP含量高，中藥高劑量組含量最低，中藥中劑量組居中，中藥各劑量組間存在顯著性差異（P<0.05）。提示，化瘀通絡(luò)中藥可明顯降低肝纖維化大鼠血清ALT、AST、ALP水平，有助于肝纖維化的治療。各組大鼠血清ALB含量的結(jié)果顯示：與正常組相比，模型組、西藥組、中藥低、中劑量組大鼠血清ALB含量明顯降低（P<0.05）,中藥高劑量組與正常組差異無統(tǒng)計學(xué)意義；與模型組相比，西藥組、中藥高、中劑量組血清ALB含量明顯升高（P<0.05），中藥低劑量組與之差別無統(tǒng)計學(xué)意義；與西藥組相比，中藥高劑量組血清ALB含量明顯升高，中藥低劑量組血清ALB水平降低（P<0.05），中藥中劑量組與之相比差別無統(tǒng)計學(xué)意義；中藥各劑量組間血清ALB水平兩兩比較差異，其中，中藥高劑量組血清ALB含量最高，中藥中劑量組次之，中藥低劑量組含量最低（P<0.05）。提示，化瘀通絡(luò)中藥可使血清ALB水平下降趨勢得到扭轉(zhuǎn)，有助于肝纖維化的治療。4各組大鼠血清LN、Col-Ⅳ水平各組大鼠血清LN、Col-Ⅳ含量結(jié)果示：與正常組相比，模型組、西藥組、中藥低、中劑量組大鼠血清LN、Col-Ⅳ含量明顯升高（P<0.05）,中藥高劑量組與正常組差異無統(tǒng)計學(xué)意義；與模型組相比，中藥各劑量組、西藥組血清LN、Col-Ⅳ明顯降低（P<0.05）；與西藥組相比，中藥高劑量組血清LN、Col-Ⅳ含量明顯減低（P<0.05），中藥低劑量組含量較高（P<0.05），中藥中劑量組與之差異無統(tǒng)計學(xué)意義；中藥各劑量組之間相比，中藥低劑量組血清LN、Col-Ⅳ含量高，中藥高劑量組含量最低，中藥中劑量組居中，中藥各劑量組間存在顯著性差異（P<0.05）。此結(jié)果提示，化瘀通絡(luò)中藥可明顯降低肝纖維化大鼠血清LN、Col-Ⅳ含量，此可能為其治療肝纖維化的機制之一。5化瘀通絡(luò)中藥對肝纖維化大鼠Ras/ERK信號轉(zhuǎn)導(dǎo)途徑的影響5.1各組大鼠肝臟組織PDGF mRNA含量、Ras蛋白mRNA水平各組大鼠肝組織PDGF mRNA、Ras mRNA含量的表達(dá)結(jié)果顯示：與正常組相比，其余各組PDGF mRNA、Ras mRNA含量均升高（P<0.05），其中模型組含量最高，中藥低劑量組次之，西藥組、中藥中劑量組再次之，中藥高劑量組含量表達(dá)最低（P<0.05）；與模型組相比，余各組肝組織PDGFmRNA、Ras mRNA含量的表達(dá)均較低（P<0.05）；與西藥組相比，中藥中劑量組與之差異無統(tǒng)計學(xué)意義；中藥各劑量組之間比較有統(tǒng)計學(xué)意義（P<0.05），其中，中藥低劑量組表達(dá)較高，中藥中劑量組次之，中藥高劑量組肝組織PDGF mRNA、Ras mRNA含量的表達(dá)最低。5.2各組大鼠肝組織ERK1含量表達(dá)正常組大鼠肝組織中ERK1呈棕黃色弱陽性表達(dá)，主要可見于小葉中央靜脈四周、匯管區(qū)及肝索Disse腔間隙中，高倍鏡下觀察可見ERK1位于Disse間隙的肝星狀細(xì)胞胞質(zhì)中，肝細(xì)胞中未見表達(dá)；模型組大鼠肝組織中ERK1表達(dá)明顯增多、增強，匯管區(qū)、小葉中央靜脈周圍及肝小葉內(nèi)均可見大量星芒狀、條索狀ERK1陽性表達(dá)，高倍鏡下觀察可見肝星狀細(xì)胞胞質(zhì)、胞核為ERK1陽性表達(dá)的分布區(qū)域；其余西藥組、中藥各劑量組陽性染色程度較模型組明顯減輕，纖維隔內(nèi)間質(zhì)細(xì)胞及炎細(xì)胞陽性染色程度減輕，ERK1陽性細(xì)胞數(shù)目減少，其中中藥高劑量組ERK1陽性程度最低，，西藥組、中藥中劑量組之間差異無統(tǒng)計學(xué)意義（P>0.05），中藥低劑量組ERK1陽性程度較中藥高、中劑量組稍高，但較模型組較低（P<0.05）。提示，化瘀通絡(luò)中藥可明顯降低肝組織中PDGF mRNA的含量并減少Ras蛋白mRNA的表達(dá)，明顯減少大鼠肝組織ERK1的表達(dá)，顯著改善大鼠抗肝纖維化能力，此可能為其治療肝纖維化的機制之一。結(jié)論：1化瘀通絡(luò)中藥可改善肝纖維化大鼠的一般狀況，改善肝功能。2化瘀通絡(luò)中藥可明顯改善肝纖維化大鼠肝臟纖維化程度，對于肝纖維化有良好的治療作用。3化瘀通絡(luò)中藥可明顯降低肝纖維化大鼠血清LN、Col-Ⅳ水平，減少二者在肝Disse間隙沉積,從而減少ECM蓄積,此可能為其治療肝纖維化的機制之一。4化瘀通絡(luò)中藥可明顯下調(diào)肝纖維化大鼠肝臟組織中PDGF mRNA、Ras蛋白mRNA以及ERK1的表達(dá)，提示其抗肝纖維化作用可能是通過激活Ras ERK1/2信號轉(zhuǎn)導(dǎo)通路實現(xiàn)的

    Objective: hepatic fibrosis is a dynamic process and pathology whichcan developing to cirrhosis by various chronic liver disease, it’s the result ofliver fibre connective tissue over sedimentation. Liver fibrosis is self recoveryreaction of organism to chronic damage, based on the feature of liverextra-cellular matrix hyperplasia and sedimentation, blood capillarization andhepatic lobule fibrosis is shown in the hepatic sinus, liver function slack up,hyperpiesia of portal vein, such as splenomegaly, ascites… in the functionality,even worse, it develops to liver cancer and influences patients quality of lifeseriously, therefore, treatment in time and blocking the further development ofliver fibrosis is significant. Lots of cell factor participated in the generationand development of liver fibrosis, PDGF is a strongest cell factor currentlywhich promotes the HSC increase and differentiation. It irritates increase,moving and inversion to the myofibroblast of HSC strongly, inducing thegeneration of collagen, sedimentation is significant in the liver fibrosisdevelopment. The researchers at home and abroad conducted a mass of studiesfor the liver fibrosis, but blocking of liver fibrosis is still difficult in clinical,recently, Using the Chinese traditional medicine to prevent the liver fibrosisindicates the certain advantage and great potential in clinical as well as baseresearch. Chinese traditional medicine Biejiajianwan comes from Jinguiyaoluewhich wrote by Zhang zhongjing, it promotes the blood circulate, removingthe blood stasis, using the gentle method and force, attack the virus andreplenish the body both together, and covered all. Demonstrated by a greatnumber of experiment, Biejiajianwan as a kind of Chinese traditional medicinehas the function for liver fibrosis complex pathogenesis via muti-pathway and muti-target spots to anti the liver fibrosis. This researchh will take thesubcutaneous injection of CCL4method for SD mouse to establish the liverfibrosis mouse module, Biejiajianwan, which promote the blood circulate andremove the blood stasis is put in use to interpose for the model. Observing thegeneral situation of experiment animal, inspect the content of LN, Col-IV, ALT,AST, ALP, ALB. Observing the liver nosology shape, inspecting liver tissuelevel of PDGF, liver tissue Ras/extracellular signal regulated kinase，ERK1,discuss the prevent function and possible affective principle of Chinesetraditional medicine which promote the blood circulate and remove the bloodstasis. It provide the experiment basis to cure the Ganji cause byextravasated blood blocking, upright deficient which defined as basicpathogeny.Methods:48healthy male SD rats, weighted280-320g, after raising oneweek, split to6groups: control, model,the western medicine group,Biejiajianwan low dose treated, middle dose treated, high dose treated. Exceptthe control group, every rats of other groups will be injected the40%CCL4peanut oil5mL/Kg in the subcutaneous from the first day, then, inject3ml/kgevery3days, meanwhile, Biejiajianwan0.55g/（kg.d）,1.1g/（kg.d）,2.2g/（kg.d）will be given to stomach for every Chinese traditional medicine lowdose,middle dose and high dose group except the western medicine group, thecolchicin0.3mg/kg will be given to the stomach for western medicine group,once per day. At the beginning of the forth weekends, execute the rats in themodel randomly, and observe the liver nosology change, the modelsuccessfully created after sixth week from experiment. Then, medicine will begiven to the stomach of rats in each Chinese medicine group for five weeks,once per day. The distilled water is put into the stomach of rats in model groupfor11weeks. The same quantity of Peanut oil is shot to the subcutaneous ofrats in control group and distilled water is put into the stomach, once per day,at the total of6weeks, after6weeks, stop the peanut oil injection and putdistilled water in the stomach for5weeks. At the end of11thweek, abrosia12hours, arteria cruralis Blood Sampling are separated, measuring the serum ALT, AST, ALP, ALB level, detect the serum LN, Col-IV level, according toradioimmunoassay, take the liver, put the liver tissue in the4%paraformaldehyde, dyed by HE and Masson, observe liver nosology change ofmouse in each group, then,preserve the liver tissue in the refrigerator with-80degree after quick-freezing by the liquid nitrogen, inspect the content of PDGF,Ras protein level in the liver tissue in accordance with reversetranscription-polymerase chain reaction RT-PCR.Results:1General condition of experimental ratsDuring the experiment, the death happened in each group except thecontrol group,3rats dead in model group,1dead in high dose medicinetreated,2in low dose treated group and middle dose treated group,3inwestern medicine group. The weights of rats in the control group areincreasing, regulate diet, quickly react, bright eye, smoothly hair. Thecondition of the rats in other groups are poor, slow in reacting, loose hair anddamp, dull color, some of the rats in the model group blind and bad temper,skinny figure.2The pathology changes of the rat,hepatic tissue in different groupIn the normal group, integrate diolame, light red color, smooth and tidysection. hepatic lobule structure clear, most of the liver cell are mononuclear,strip liver surface, liver cell shows radial pattern and surround the center vein.Regarding on the model group, the shape of liver is abnormally, coarse surface,feels like the sand particle. Liver cell become swell and getting bigger, most ofcells thanatosis, the vessels expanded and inflammation cells found, thestructure of liver lobule damaged, fibers connective tissue in the veins area hasobvious hyperplasia, the liver lobule is surrounded to different size of livercell bunches and take shape to pseudolobule. The liver lobule in the each doseof Chinese medicine group which can promote the blood circulate and removethe stasis has clear structure. Liver sinus expand slightly, liver cells necrotic.fibers connective tissue in the veins area has moderate hyperplasia, theformation of pseudolobule decreasing distinctly, the liver nosology change of western medicine group is in the middle of western group and Chinesemedicine group. Note: the medicine which promote the blood circulate andremove the stasis improves the liver fibrosis level in the liver fibrosis rats, andfunction better than western medicine colchicines.3The levels of ALT, AST, ALP, and ALB in the serum of different groupsThe level of ALT, AST and ALP in the serum of different groups display:compared with normal group, the content of ALT, AST, ALP in the rats serumincrease obviously （P<0.05） in model group, western medicine group andChinese medicine low dose group and Chinese medicine middle dose group,and there were no significant differences between the normal group andChinese medicine high dose treated group （p>0.05）. Compare with modelgroup, the serum ALT, AST, ALP content decrease obviously （P<0.05） in eachChinese medicine group and western medicine group. Compare with westernmedicine group, serum ALT, AST, ALP content decrease obviously （P<0.05）in Chinese medicine high dose treated group, more content in the Chinesemedicine low dose treated group（P<0.05）, there is no meaning to comparewith Chinese medicine middle dose treated group（p>0.05）. compare in eachgroups of Chinese medicine group, the serum ALT、AST、ALP content mostthan others in low dose group, and the serum ALT、AST、ALP content lest thanothers in high dose group, the middle dose group in the middle place, distinctdiscrepancy exist in each Chinese medicine dose group （P<0.05）, Note: theChinese traditional medicine which promote the blood circulate and removethe stasis can decrease serum ALT, AST, ALP level of live fibrosis rats,improve the treatment of liver fibrosis.The level of ALB in the serum of different groups display: comparedwith normal group, the serum ALB declined obviously （P<0.05） in modelgroup, western medicine group, Chinese medicine low dose treated group andmiddle dose treated group, and there were no significant differences betweenthe normal group and Chinese medicine high dose treated group （p>0.05）.compared with model group, serum ALB content increase obviously （P<0.05）in western medicine group, Chinese medicine high dose treated group and middle dose group, There isn’t any meaning to compare discrepancy withChinese medicine low dose treated group. compared with western medicinegroup, serum ALB content increase obviously in Chinese medicine high dosetreated group, serum ALB content level decrease （P<0.05） in Chinesemedicine middle dose treated group, there isn’t any meaning to compare withmiddle dose group. there is discrepancy existing among each Chinesemedicine group serum ALB level. There into, high dose treated has the mostserum ALB content, middle dose group follows in second place, low dosetreated group has the lowest content （P<0.05）, Note: the medicine whichpromote the blood circulate and remove the stasis can turnaround the serumALB level decreasing trend, and improve the treatment for liver fibrosis.4The levels of LN and Col-IV in the serum of different groupsThe rats serum LN, Col-IV content indicates: compare with normal group,rats serum LN, Col-IV content in model, western, Chinese medicine low doseand middle dose increase obviously （P<0.05）,, and there were no significantdifferences between the normal group and Chinese medicine high dose treatedgroup （p>0.05）. compare with western medicine, the serum LN, Col-IVcontent decrease obviously （P<0.05） in the Chinese medicine high dose treatedgroup, higher content level in low dose medicine group （P<0.05）, no meaningto compare the discrepancy with middle dose treated group（p>0.05）. compareamong each dose medicine treated group, serum in Chinese medicine low dosetreated group has highest LN, Col-IV content, high dose group has lowestcontent and middle dose group in the middle place, the obvious discrepancyexisting in each dose treated medicine group（P<0.05）.The conclusion indicatesthat Chinese traditional medicine which promote the blood circulate andremove stasis can decrease the liver fibrosis serum LN, Col-IV content, maybethis is one of the method which treat liver fibrosis.5The levels of PDGF mRNA and Ras protein mRNA in the hepatic tissue ofdifferent groupsLiver tissue PDGF mRNA, Ras mRNA content indicates: compare withnormal group, the content of PDGF mRNA, Ras mRNA increased （P<0.05） in each group, thereinto, model group has the highest content, Chinese medicinelow dose group follows, western medicine, middle dose group at the middleplace, high dose group has the lowest content （P<0.05）. compare with themodel group, PDGF mRNA, Ras mRNA is shown as lower content in the livertissue, compare with western medicine, no meaning to compare thediscrepancy with Chinese medicine middle dose group（P>0.05）. compareamong each dose Chinese medicine group has the statistics meaning（P<0.05）,thereinto, low dose has the highest content, middle dose follows, liver tissuehas lowest PDGF mRNA, Ras mRNA content in the Chinese medicine highdose treated group.6The ERK1content of liver tissue in each group indicationIn the normal group, ERK1in liver tissue shows slight positive andyellow color, especially surround center vein of liver lobule, manifold, andDisse chamber, ERK1in the cytoplasm of liver star shape cells observed byhigh-power microscope, liver cells aren’t shown; regarding on the modelgroup, ERK1in the liver tissue increasing, enhance, ERK1as star shape andstrip shape is shows as positive surrounding the center vein and in the liverlobule，positive ERK1is shown in the area of the liver star cytoplasm andnucleus observed by high-power microscope, in western medicine and eachChinese medicine group, positive dyeing degree is decreased obviously,dyeing degree of interstitial cell between fibro and inflammatory cells positivedecrease, quantity of ERK1positive cells decrease, thereinto, ERK1has thelowest positive in Chinese medicine high dose group, and the highest positivein Chinese medicine low dose group, and no meaning to compare amongwestern and Chinese medicine middle dose. Note: the Chinese medicine whichpromote the blood circulate and remove stasis can decrease the PDGF mRNAcontent in the liver tissue and decrease the mRNA in the Ras protein obviously,decrease the ERK1in the rats liver tissue, improve the capability of anti liverfibrosis, maybe this is one of methods to cure liver fibrosis.Conclusions:1The Chinese traditional medicine which promote blood circulate and remove the stasis can improve the general situation of rats liver fibrosis, andimprove the liver function.2The Chinese traditional medicine which promote the blood circulateand remove the stasis can improve the rats liver fibrosis level, it’s a welltreatment for liver fibrosis.3The Chinese traditional medicine which promote blood circulate andremove the stasis can decrease the serum LN, Col-IV levels, decrease thesedimentation in the liver Disse, therefore, decrease the accumulation of ECM,maybe this is one of methods to cure liver fibrosis.4The Chinese traditional medicine which promote blood circulate andremove the stasis can decrease the PDGF mRNA and Ras protein mRNA inthe liver tissue of liver fibrosis, decrease the ERK1in the rats liver tissueobviously, improve the capacity anti liver fibrosis via activateRas/ERK1/2signal transmit route to achieve this, maybe this is one of themethods to prevent liver fibrosis.

        化瘀通絡(luò)中藥對肝纖維化大鼠的治療作用及其對血小板衍生生長因子（PDGF）及Ras信號轉(zhuǎn)導(dǎo)通路的影響

中文摘要4-9英文摘要9-15前言16-17材料與方法17-24結(jié)果24-28附圖28-37附表37-40討論40-49結(jié)論49-50參考文獻(xiàn)50-54綜述 血小板衍生生長因子在肝纖維化發(fā)生中的作用54-64    參考文獻(xiàn)59-64致謝64-65個人簡歷65

  本文關(guān)鍵詞：化瘀通絡(luò)中藥對肝纖維化大鼠的治療作用及其對血小板衍生生長因子（PDGF）及Ras信號轉(zhuǎn)導(dǎo)通路的影響，由筆耕文化傳播整理發(fā)布。