目的：觀察經(jīng)導(dǎo)管射頻消融去腎交感神經(jīng)術(shù)（RDN）對高血壓犬心肌纖維化程度、心肌miR-133a、結(jié)締組織生長因子（CTGF）表達的改變,探討RDN對心肌纖維化的影響及可能的機制。方法：1、用高脂飲食建立高血壓犬模型,將28只比格犬（10-12月齡）隨機分為對照組（普通飲食,n=6）和模型組（高脂飲食,n=22）。2、建模12周后將建模成功的高血壓犬（n=20）隨機分為手術(shù)組（n=10）及假手術(shù)組（n=10）,手術(shù)組行RDN術(shù),假手術(shù)組僅行腎動脈造影。3、監(jiān)測實驗犬體重、血壓,術(shù)后6月無痛處死實驗犬,取心臟組織,行Masson染色觀察心肌纖維化程度,計算膠原容積分數(shù)（CVF）、周圍血管膠原面積比率（PVCA）, Real-time PCR檢測組織miR-133a表達水平；Real-time PCR、Western-blot檢測組織CTGF表達。結(jié)果：1、建模期假手術(shù)組、手術(shù)組實驗犬體重、收縮壓（SBP）、舒張壓（DBP）、平均動脈壓（MAP）均明顯升高,與基線、對照組相比均有統(tǒng)計學(xué)差異（P<0.05）,假手術(shù)組、手術(shù)組組間對比無統(tǒng)計學(xué)差異（P>0.05）；2、經(jīng)導(dǎo)管射頻消融去... 

【文章來源】：中南大學(xué)湖南省211工程院校985工程院校教育部直屬院校

【文章頁數(shù)】：65 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Contents
ABBREVIATION
CHAPTER 1 INTRODUCTION
CHAPTER 2 MATERIALS AND METHODS
    2.1 MATERIALS
        2.1.1 ANIMALS
        2.1.2 FEEDING
        2.1.3 IMPORTANT REAGENTS
        2.1.4 IMPORTANT EQUIPMENT AND SETTINGS
        2.1.5 MAIN REACTANTS PREPAR ATION
    2.2 METHOD OF STUDY
        2.2.1 GROUPING
        2.2.2 BLOOD PRESSURE AND BODY WEIGHT MEASUREMENT
        2.2.3 RENAL SYMPATHETIC DENERVATION INTERVENTION
        2.2.4 ECHOCARDIOGRAPHY
        2.2.5 MEASUREMENT OF HEART WEIGHT AND LVMI
        2.2.6 CARDIAC TISSUE PREPARATION
        2.2.7 MASSON STAINING OF HEART TISSUE
        2.2.8 RNA EXTRACTION
        2.2.9 DETECTION OF MIRNA WITH QPCR
        2.2.10 WESTERN BLOT
    2.3 STATISTICAL ANALYSIS
CHAPTER 3 RESULTS
    3.1 GENERAL DATA
    3.2 THE COMPARISON OF BODY WEIGHT AND BLOOD PRESSUREBETWEEN
        3.2.1 THE COMPARISON OF BODY WEIGHT BETWEEN GROUPS
        3.2.2 THE COMPARISON OF BLOOD PRESSURE BETWEEN GROUPS
    3.3 THE EFFECT OF RDN ON LVM AND LVMI
        3.3.1 THE EFFECT OF RDN ON LVM
        3.3.2 THE EFFECT OF RDN ON LVMI
    3.4 THE EFFECT OF RDN ON CARDIAC FIBROSIS
    3.5 THE EFFECT OF RDN ON MYOCARDIAL MIR-133A GENEEXPRESSION
    3.6 THE EFFECT OF RDN ON CTGF EXPRESSION
CHAPTER 4 DISCUSSION
    4.1 THE EFFECT OF RDN ON BLOOD PRESSURE
    4.2 THE EFFECT OF RDN ON CARDIAC FIBROSIS
        4.2.1 THE PATHOGENESIS OF MYOCARDIAL FIBROSIS IN HYPERTENSION
        4.2.2 RECENT ADVANCE IN THE TREATMENT OF CARDIAC REMODELING
        4.2.3 THE EFFECT OF RDN ON CARDIAC FIBROSIS
    4.3 THE POSSIBLE MECHANISM INVOLVED IN THE IMPROVEMENT OFCARDIAC FIBROSIS FOLLOWING RDN
        4.3.1 THE EXPRESSION OF MYOCARDIAL MIR-133A AND CTGF INCARDIAC FIBROSIS
        4.3.2 THE EFFECT OF RDN ON MYOCARDIAL MIR-133A-CTGF PATHWAY
    4.4 LIMITATION OF THE STUDY
CHAPTER 5 CONCLUSION
REFERENCES
REVIEW ARTICLE
    References
Acknowledgement


【參考文獻】：
期刊論文
[1]自發(fā)性高血壓大鼠心肌組織microRNA-133a與TGF-β1蛋白表達的改變及意義[J]. 譚文鵬,楊侃,陳晞明,陳次濱.  中國病理生理雜志. 2013(04)
[2]MicroRNA-1在心肌肥大中對L-型鈣通道β2亞基的負性調(diào)控作用[J]. 吳揚,耿鵬,王玉琴,劉艷.  中國應(yīng)用生理學(xué)雜志. 2012(04)



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