【摘要】：背景:研究表明,復(fù)合使用人工組織工程骨材料和脫細(xì)胞骨基質(zhì)做為骨缺損修復(fù)的支架材料,可以綜合兩者的優(yōu)點(diǎn)。目的:進(jìn)一步驗(yàn)證脂肪組織基質(zhì)血管基質(zhì)性成分聯(lián)合脫細(xì)胞骨基質(zhì)殼聚糖支架復(fù)合物修復(fù)兔橈骨缺損的安全性和生物相容性。方法:取38只新西蘭大白兔,其中3只用于脂肪組織血管基質(zhì)成分的制備,3只用于脫細(xì)胞骨基質(zhì)的制備,32只分為實(shí)驗(yàn)組和對照組,采用Brownlow法制備橈骨缺損型,實(shí)驗(yàn)組移植血管基質(zhì)成分-脫細(xì)胞骨基質(zhì)殼聚糖復(fù)合支架,對照組造模后不做任何處理。術(shù)后2,4個月分別進(jìn)行一般情況和大體觀察、X射線拍照、病理觀察、Lane-Sandhu組織學(xué)評分。結(jié)果與結(jié)論:1術(shù)后2,4個月2組無感染現(xiàn)象,但實(shí)驗(yàn)組的活動量和愈合程度顯著性好于對照組;2術(shù)后2個月實(shí)驗(yàn)組骨缺損部位的X射線影像結(jié)果有顯著灰白色的高密度影,術(shù)后4個月與正常的橈骨骨組織相同,對照組骨不連接;3術(shù)后2,4個月實(shí)驗(yàn)組病理顯示骨組織生長情況顯著性的高于對照組,Lane-Sandhu組織學(xué)評分顯著性的高于對照組(P0.05);4結(jié)果說明,血管基質(zhì)成分-脫細(xì)胞骨基質(zhì)殼聚糖復(fù)合支架修復(fù)兔橈骨缺損具有很好的安全性和生物相容性。
[Abstract]:Background: studies have shown that the composite use of artificial tissue engineering bone materials and acellular bone matrix as scaffold materials for bone defect repair can integrate the advantages of both. Aim: to further verify the safety and biocompatibility of adipose tissue matrix vascular matrix composition combined with acellular bone matrix chitosan scaffold for repairing rabbit radius defect. Methods: 38 New Zealand white rabbits were divided into experimental group and control group. Among them, 3 rabbits were used for the preparation of vascular matrix in adipose tissue. 32 rabbits were divided into experimental group and control group. Radial defect type was prepared by Brownlow method. The experimental group was implanted with acellular bone matrix chitosan composite scaffold, while the control group did not do any treatment after modeling. The general and gross observations were taken 2 and 4 months after operation, and the histological score of Lane-Sandhu was observed by pathology. Results and conclusion there was no infection in the two groups at 2 and 4 months after operation, but the activity and healing degree of the experimental group was significantly better than that of the control group 2 months after operation. At 4 months after operation, the radial bone tissue of the control group was the same as that of the normal radial bone tissue. The pathological tissue growth of the experimental group was significantly higher than that of the control group (P0.05) at 2 and 4 months after the operation, and the histological score of the control group was significantly higher than that of the control group (P0.05). It is safe and biocompatible to repair rabbit radius defect with vascular matrix component acellular bone matrix chitosan composite scaffold.
【作者單位】： 解放軍第四五五醫(yī)院骨科;解放軍第四五五醫(yī)院生物診療科;解放軍第四五五醫(yī)院整形外科;
【基金】：全軍醫(yī)藥衛(wèi)生科研項(xiàng)目(14ZD09)~~
【分類號】：R318.08;R683

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