【摘要】：背景:研究表明,復(fù)合使用人工組織工程骨材料和脫細(xì)胞骨基質(zhì)做為骨缺損修復(fù)的支架材料,可以綜合兩者的優(yōu)點。目的:進一步驗證脂肪組織基質(zhì)血管基質(zhì)性成分聯(lián)合脫細(xì)胞骨基質(zhì)殼聚糖支架復(fù)合物修復(fù)兔橈骨缺損的安全性和生物相容性。方法:取38只新西蘭大白兔,其中3只用于脂肪組織血管基質(zhì)成分的制備,3只用于脫細(xì)胞骨基質(zhì)的制備,32只分為實驗組和對照組,采用Brownlow法制備橈骨缺損型,實驗組移植血管基質(zhì)成分-脫細(xì)胞骨基質(zhì)殼聚糖復(fù)合支架,對照組造模后不做任何處理。術(shù)后2,4個月分別進行一般情況和大體觀察、X射線拍照、病理觀察、Lane-Sandhu組織學(xué)評分。結(jié)果與結(jié)論:1術(shù)后2,4個月2組無感染現(xiàn)象,但實驗組的活動量和愈合程度顯著性好于對照組;2術(shù)后2個月實驗組骨缺損部位的X射線影像結(jié)果有顯著灰白色的高密度影,術(shù)后4個月與正常的橈骨骨組織相同,對照組骨不連接;3術(shù)后2,4個月實驗組病理顯示骨組織生長情況顯著性的高于對照組,Lane-Sandhu組織學(xué)評分顯著性的高于對照組(P0.05);4結(jié)果說明,血管基質(zhì)成分-脫細(xì)胞骨基質(zhì)殼聚糖復(fù)合支架修復(fù)兔橈骨缺損具有很好的安全性和生物相容性。
[Abstract]:Background: studies have shown that the composite use of artificial tissue engineering bone materials and acellular bone matrix as scaffold materials for bone defect repair can integrate the advantages of both. Aim: to further verify the safety and biocompatibility of adipose tissue matrix vascular matrix composition combined with acellular bone matrix chitosan scaffold for repairing rabbit radius defect. Methods: 38 New Zealand white rabbits were divided into experimental group and control group. Among them, 3 rabbits were used for the preparation of vascular matrix in adipose tissue. 32 rabbits were divided into experimental group and control group. Radial defect type was prepared by Brownlow method. The experimental group was implanted with acellular bone matrix chitosan composite scaffold, while the control group did not do any treatment after modeling. The general and gross observations were taken 2 and 4 months after operation, and the histological score of Lane-Sandhu was observed by pathology. Results and conclusion there was no infection in the two groups at 2 and 4 months after operation, but the activity and healing degree of the experimental group was significantly better than that of the control group 2 months after operation. At 4 months after operation, the radial bone tissue of the control group was the same as that of the normal radial bone tissue. The pathological tissue growth of the experimental group was significantly higher than that of the control group (P0.05) at 2 and 4 months after the operation, and the histological score of the control group was significantly higher than that of the control group (P0.05). It is safe and biocompatible to repair rabbit radius defect with vascular matrix component acellular bone matrix chitosan composite scaffold.
【作者單位】： 解放軍第四五五醫(yī)院骨科;解放軍第四五五醫(yī)院生物診療科;解放軍第四五五醫(yī)院整形外科;
【基金】：全軍醫(yī)藥衛(wèi)生科研項目(14ZD09)~~
【分類號】：R318.08;R683

【相似文獻】

相關(guān)期刊論文 前10條

1 鄧遲,王遠(yuǎn)亮,李雪梅,鄧敏,夏烈文;骨基質(zhì)結(jié)構(gòu)和力學(xué)性能的比較研究[J];樂山師范學(xué)院學(xué)報;2004年12期

2 史增敦;莫東旭;柴本甫;;骨基質(zhì)誘導(dǎo)的軟骨和骨形成[J];國外醫(yī)學(xué).創(chuàng)傷與外科基本問題分冊;1986年04期

3 史增敦,莫東旭;骨基質(zhì)誘導(dǎo)的軟骨和骨形成[J];西安交通大學(xué)學(xué)報(醫(yī)學(xué)版);1985年02期

4 朱謹(jǐn);歐陽欣;許彪;;豬髂骨不同鈣含量骨基質(zhì)的生物學(xué)性質(zhì)的初步檢測[J];昆明醫(yī)學(xué)院學(xué)報;2009年09期

5 王偉;李彥林;施永彥;李錦華;趙宙;曾小軍;;兔脫鈣骨基質(zhì)材料復(fù)合骨髓間充質(zhì)干細(xì)胞的體外培養(yǎng)[J];鄖陽醫(yī)學(xué)院學(xué)報;2010年01期

6 孫新君,王正國,朱佩芳,周萍,張宇,劉大維,張猛;脫細(xì)胞異種骨基質(zhì)植入后受體外周血T淋巴細(xì)胞亞型的變化[J];中國修復(fù)重建外科雜志;2005年04期

7 孫曉雷;馬信龍;馬劍雄;張_";王志剛;王濤;張華鋒;;牛脫細(xì)胞骨基質(zhì)的生物力學(xué)特性及其黏附性[J];中國組織工程研究與臨床康復(fù);2008年49期

8 李康杰;孫抒;;去除移植免疫反應(yīng)抗原成分脫細(xì)胞天然骨基質(zhì)的制備[J];中國組織工程研究與臨床康復(fù);2011年08期

9 李康杰;孫抒;;脫細(xì)胞天然骨基質(zhì)與誘導(dǎo)性成骨細(xì)胞的體外相容性[J];中國組織工程研究與臨床康復(fù);2011年12期

10 袁勁松;;高效型健骨因子改善骨病效果明顯[J];中國保健營養(yǎng);2009年05期

相關(guān)會議論文 前5條

1 張東輝;許永華;許琴;李建瑛;是文輝;李佳佳;盧開伯;李曉晶;馬娜;龍志新;;兔脫細(xì)胞骨基質(zhì)體內(nèi)埋植一年病理觀察[A];第九屆中國實驗動物科學(xué)年會（2010新疆）論文集[C];2010年

2 田志逢;劉喜民;張小玲;王豐剛;;煅燒骨基質(zhì)/骨基質(zhì)明膠重組人工骨對大鼠骨髓間充質(zhì)干細(xì)胞成骨潛能的影響[A];中國解剖學(xué)會2012年年會論文文摘匯編[C];2012年

3 李稚君;馬信龍;馬劍雄;孫曉雷;張園;;不同預(yù)濕處理方法對大鼠骨髓間充質(zhì)干細(xì)胞復(fù)合牛脫細(xì)胞骨基質(zhì)培養(yǎng)的影響[A];第九屆全國生物力學(xué)學(xué)術(shù)會議論文匯編[C];2009年

4 李稚君;馬信龍;馬劍雄;孫曉雷;張園;趙秀寶;;不同預(yù)濕處理方法對Wistar大鼠骨髓間充質(zhì)干細(xì)胞復(fù)合牛脫細(xì)胞骨基質(zhì)培養(yǎng)的影響[A];2009第十七屆全國中西醫(yī)結(jié)合骨傷科學(xué)術(shù)研討會論文匯編[C];2009年

5 孫，

本文編號：2216740