【摘要】：背景:細(xì)胞內(nèi)皮化的流動剪切力大小和作用方式是小口徑組織工程血管的構(gòu)建中的關(guān)鍵問題。小口徑組織工程血管構(gòu)建后的抗血流沖擊能力是決定移植成功的關(guān)鍵因素之一,較多人把研究重點集中在如何制作移植血管支架材料,如何提高宿主細(xì)胞種植后其血管內(nèi)壁的抗凝效果,而對細(xì)胞內(nèi)皮層的力學(xué)馴化則關(guān)注較少,該方法有可能是提高組織工程血管移植成功率的有效手段之一。目的:比較單一水平和成梯度增加的流動剪切力對組織工程血管內(nèi)皮化的影響,獲得適用于組織工程血管內(nèi)皮化的剪切力大小和加載方式。方法:采用血管鋪片硝酸銀染色法和內(nèi)皮細(xì)胞肌動蛋白熒光標(biāo)記法,觀察組織工程血管內(nèi)壁上內(nèi)皮細(xì)胞的數(shù)量、形狀和黏附性能等變化情況。結(jié)果與結(jié)論:經(jīng)2.5和3.0 N/m~2剪切力作用2 h后,血管內(nèi)壁上的內(nèi)皮細(xì)胞幾乎全部脫落;而經(jīng)0.5-3.0 N/m~2,每2 h增加0.2 N/m~2的成梯度增加的剪切力作用后,血管內(nèi)皮細(xì)胞層保持較完整,細(xì)胞形態(tài)成明顯的軸向排列,且能誘導(dǎo)內(nèi)皮細(xì)胞中應(yīng)力纖維的出現(xiàn)。說明成梯度增加的流動剪切力這種"馴化"方式,能較好地促進小口徑組織工程血管的內(nèi)皮化。
[Abstract]:Background: the size and mode of flow shear force of endothelial cells are the key problems in the construction of small diameter tissue engineering blood vessels. The ability of small caliber tissue engineering blood vessel to resist blood flow impact is one of the key factors to determine the success of transplantation. How to improve the anticoagulant effect of host cells after implantation, but less attention to the mechanical acclimation of the intracellular cortex, this method may be one of the effective methods to improve the success rate of tissue engineering vascular transplantation. Aim: to compare the effects of single level and gradient increased flow shear stress on vascular endothelization in tissue engineering, and obtain the shear force and loading mode suitable for tissue engineering vascular endothelialization. Methods: the number, shape and adhesion of endothelial cells on the inner wall of tissue engineered vessels were observed by silver nitrate staining and fluorescence labeling of actin in endothelial cells. Results and conclusion: after 2 h and 3.0 N/m~2 shear stress, almost all of the endothelial cells on the inner wall of the vessel shed off, but after the addition of 0.2 N/m~2 / 2 of shear stress every 2 h, the vascular endothelial cell layer remained intact. The morphology of the cells formed an obvious axial arrangement and induced the appearance of stress fibers in endothelial cells. The results show that the "acclimation" of flow shear force in gradient can promote the endothelialization of small caliber tissue engineering vessels.
【作者單位】： 冠昊生物科技股份有限公司再生型醫(yī)用植入器械國家工程實驗室;廣東省體育科學(xué)研究所;
【基金】：廣東省重大科技專項(2010A080407005) 國家重點研究計劃生物醫(yī)用材料研發(fā)與組織器官修復(fù)替代重點專項(2016YFC1103202)~~
【分類號】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曹雪飛;董國;楊樹森;;剪切力對血管內(nèi)皮功能影響及機制研究進展[J];中華實用診斷與治療雜志;2016年10期

2 柴守棟;顧承雄;;血流剪切力對靜脈橋血管再狹窄影響的研究進展[J];心肺血管病雜志;2016年06期

3 姜力;陳思原;王園園;王淑芳;;小口徑人工血管構(gòu)建研究現(xiàn)狀與展望[J];中國生物醫(yī)學(xué)工程學(xué)報;2016年03期

4 王秋蘭;;腦血管支架種類及材料學(xué)特點與支架置入后的補體反應(yīng)及干預(yù)[J];中國醫(yī)學(xué)工程;2016年02期

5 汪濤;趙s，

本文編號：2194756