【摘要】：為了改善聚氨酯的血液相容性能，本文主要研究了利用微波輔助表面接枝改性的方法，對(duì)聚氨酯的表面進(jìn)行活化并接枝上不同分子量的聚乙二醇（PEG/MPEG/APEG）以及肝素，利用傅里葉變換衰減全反射紅外光譜法、表面水接觸角、掃描電鏡和原子力顯微鏡對(duì)聚氨酯表面特征進(jìn)行分析，并通過(guò)溶血率、凝血酶原時(shí)間、補(bǔ)體激活等測(cè)試考察了聚氨酯表面的血液相容性能。 采用微波輔助技術(shù)將聚氨酯膜的表面進(jìn)行活化。結(jié)果表明：微波輔助反應(yīng)能明顯加快聚氨酯表面活化的速度，，其接枝效果受微波功率、反應(yīng)時(shí)間以及反應(yīng)物體積質(zhì)量分?jǐn)?shù)的影響。在本實(shí)驗(yàn)條件下，微波功率為100W，反應(yīng)物的質(zhì)量分?jǐn)?shù)為5%，反應(yīng)時(shí)間為10分鐘時(shí)，微波輔助表面活化的效果以及效益最好。SEM表面分析表明，與傳統(tǒng)加熱反應(yīng)方法相比，微波輔助反應(yīng)對(duì)膜表面的破壞程度更小。 采用微波輔助技術(shù)在聚氨酯膜的表面接枝不同分子量的PEG/MPEG，并與傳統(tǒng)加熱接枝PEG/MPEG的方法進(jìn)行對(duì)比。通過(guò)ATR-FTIR、接觸角分析不同方法改性后聚氨酯表面特性的變化，通過(guò)SEM、AFM觀察不同改性方法對(duì)聚氨酯表面形貌的影響，通過(guò)溶血率、血小板黏附測(cè)試、凝血時(shí)間以及補(bǔ)體激活測(cè)試考察不同改性方法對(duì)聚氨酯的血液相容性能的影響。結(jié)果表明：微波輔助反應(yīng)能明顯加快聚氨酯表面接枝PEG/MPEG的速度，但對(duì)接枝量沒(méi)有太大的影響。SEM和AFM表面分析表明，與傳統(tǒng)加熱反應(yīng)方法相比，微波輔助反應(yīng)對(duì)膜表面的破壞程度更小，膜表面更加光滑平整。水接觸角的結(jié)果也表明，微波輔助反應(yīng)接枝PEG/MPEG所得膜表面具有良好的親水性能。血液相容性試驗(yàn)證實(shí)，利用微波輔助改性的聚氨酯表面呈現(xiàn)出更加優(yōu)異的血液相容性。 采用微波輔助技術(shù)將聚氨酯表面活化后，再將APEG接枝到聚氨酯上，最后利用EDC·HCl和NHS活化接枝上肝素。通過(guò)聚氨酯表面肝素化后性能的改變以及血液相容性能的改善來(lái)研究肝素對(duì)聚氨酯表面性能的影響。血液相容性測(cè)試結(jié)果證實(shí)肝素對(duì)材料血液相容性有明顯的改善作用。
[Abstract]:In order to improve the blood compatibility of polyurethane, the surface of polyurethane was activated and grafted with poly (ethylene glycol) (PEG/MPEG/APEG) and heparin (heparin) with different molecular weight by microwave assisted surface grafting. Fourier transform attenuated total reflectance infrared spectroscopy (FTIR), surface water contact angle, scanning electron microscope (SEM) and atomic force microscope (AFM) were used to analyze the surface characteristics of polyurethane. The blood compatibility of polyurethane surface was investigated by complement activation test. The surface of polyurethane film was activated by microwave assisted technique. The results showed that microwave-assisted reaction could accelerate the activation of polyurethane surface, and the grafting effect was influenced by microwave power, reaction time and mass fraction of reactants. Under the experimental conditions, microwave power is 100 W, mass fraction of reactant is 5, reaction time is 10 minutes, the effect and benefit of microwave assisted surface activation is the best. SEM surface analysis shows that compared with traditional heating reaction method, microwave assisted surface activation is better than conventional heating reaction method. The microwave assisted reaction has less damage to the film surface. The microwave-assisted technique was used to graft PEG- / MPEGon with different molecular weights on the surface of polyurethane membrane and compared with the conventional heating grafted PEG/MPEG. The surface properties of polyurethane modified by different methods were analyzed by ATR-FTIR.The effects of different modification methods on the surface morphology of polyurethane were observed by SEMAFM. The hemolysis rate and platelet adhesion were measured. The effects of different modification methods on the blood compatibility of polyurethane were investigated by coagulation time and complement activation test. The results show that the microwave-assisted reaction can obviously accelerate the grafting rate of PEG/MPEG on polyurethane surface, but the grafting amount has no great influence on AFM surface analysis. The microwave assisted reaction has less damage to the film surface, and the film surface is more smooth and smooth. The results of water contact angle also showed that the surface of the membrane prepared by microwave-assisted reaction grafted with PEG/MPEG had good hydrophilicity. Hemocompatibility test confirmed that the surface of polyurethane modified by microwave showed better blood compatibility. The surface of polyurethane was activated by microwave-assisted technique, then APEG was grafted onto polyurethane. Finally, EDC HCl and NHS were used to activate the grafted heparin. The effects of heparin on the surface properties of polyurethane were studied through the changes of its properties after heparinization and the improvement of its blood compatibility. The results of blood compatibility test confirmed that heparin had a significant effect on improving the blood compatibility of the materials.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R318.08

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