本文關(guān)鍵詞：組織工程骨修復(fù)骨缺損的研究進(jìn)展及臨床應(yīng)用，由筆耕文化傳播整理發(fā)布。

骨缺損

中國組織工程研究與臨床康復(fù) 第14卷 第24期 2010–06–11出版

Journal of Clinical Rehabilitative Tissue Engineering Research June 11, 2010 Vol.14, No.24

組織工程骨修復(fù)骨缺損的研究進(jìn)展及臨床應(yīng)用★

陳 欣，張春林

Research progress and clinical application of repairing bone defects using tissue engineered bone

Chen Xin, Zhang Chun-lin

Abstract

Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China;

Chen Xin★, Studying for master’s degree, Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China seahorse127@

Correspondence to: Zhang Chun-lin, Associate professor, Associate chief physician, Department of Orthopedics, the Sixth People’s

Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China shzhangchunlin@

Received: 2010-01-16 Accepted: 2010-02-09

BACKGROUND: With the development of biotechnology, repairing bone defects using tissue engineered bone has become a hot spot.

OBJECTIVE: To overview the research progress and clinical applications of repairing bone defects with tissue engineered bone. METHODS: The databases of PubMed, Springer Link, and Science Direct were retrieved for papers published from January 2000 to March 2009 with key words of “tissue engineering of bone, bone defect, and clinical applications”. The relevant papers

concerning stem cell, scaffold material, animal model, vascularization during repairing bone defects with tissue engineered bone were collected.

RESULTS AND CONCLUSION: Bone defect reparation has been a clinical challenge. The traditional autogenous bone

transplantation was the gold standard for local bone defects, which had drawback of complication. It is an ideal technique that applying allogeneic bone scaffolds without number restrictions to repair bone defects, which had possibility of rejection reaction and infection. Therefore, the applications of tissue engineered bone are the tendency. The tissue engineered bone comprised 4 key elements: bone conductive scaffolds, releasing osteogenic growth factor, loaded osteogenic potential cells, as well as vascularization. Thus, the factors involved in osteanagenesis and its relations, as well as animal model studies should be evaluated during bone defects reparation. There have been shown positive roles and considerable development prospects of tissue engineered bone, but many issues have to be solved before the clinical applications.

Chen X, Zhang CL. Research progress and clinical application of repairing bone defects using tissue engineered bone.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(24):4486-4490. [ ]

摘要

背景：隨著生物技術(shù)的興起，以生物材料來制作移植替代物的組織工程學(xué)正在不斷地發(fā)展，因此組織工程骨修復(fù)骨缺損也成為當(dāng)前的研究熱點。

目的：概述國際上關(guān)于骨組織工程研究的現(xiàn)狀及臨床應(yīng)用的進(jìn)展。

方法：應(yīng)用計算機(jī)檢索PubMed數(shù)據(jù)庫、Springer Link數(shù)據(jù)庫、Science Direct數(shù)據(jù)庫2000-01/2009-03文獻(xiàn)，檢索詞為 “tissue engineering of bone，bone defect，clinical applications”。選擇與組織工程骨修復(fù)骨缺損相關(guān)的種子細(xì)胞、支架材料、動物模型、血管化和臨床應(yīng)用的文章， 無論觀察對象為人或動物均納入檢索標(biāo)準(zhǔn)。

結(jié)果與結(jié)論：骨缺損的修復(fù)是臨床上的難題之一, 傳統(tǒng)吻合血管的自體骨移植是治療局部骨缺損的金標(biāo)準(zhǔn)，但是其存在供骨區(qū)并發(fā)癥及數(shù)量有限的缺點。而無活性的異體骨雖然不受數(shù)量的限制，但是存在排異和傳染疾病的可能。因此利用組織工程技術(shù)構(gòu)建組織工程骨是修復(fù)骨缺損的趨勢。組織工程骨包含具有骨傳導(dǎo)性的支架、釋放誘導(dǎo)成骨的生長因子、負(fù)載具有成骨潛能的細(xì)胞及組織工程骨血管化或充足的血供4個關(guān)鍵元素，因此要準(zhǔn)確地評估組織工程骨修復(fù)骨缺損的作用，需要進(jìn)一步理解參與骨再生的各種因素及其相互作用，同時還要在標(biāo)準(zhǔn)化動物模型研究中衡量積極因素和可能的有害影響。骨組織工程在實驗研究領(lǐng)域已經(jīng)顯示出了積極的作用和相當(dāng)大的發(fā)展前景，但在進(jìn)入臨床應(yīng)用以前還有很多問題等待解決。 關(guān)鍵詞：組織工程骨；骨缺損；臨床應(yīng)用；修復(fù)；血管化；種子細(xì)胞 doi:10.3969/j.issn.1673-8225.2010.24.027

陳欣，張春林．組織工程骨修復(fù)骨缺損的研究進(jìn)展及臨床應(yīng)用[J].中國組織工程研究與臨床康復(fù)，2010，14(24):4486-4490. [ ]

上海交通大學(xué)附屬第六人民醫(yī)院骨科，上海市 200233

陳 欣★，男，1984年生，浙江省東陽市人，漢族，上海交通大學(xué)在讀碩士，主要從事骨創(chuàng)傷與骨組織工程的研究。 seahorse127@

通訊作者：張春林，副教授，副主任醫(yī)師，上海交通大學(xué)附屬第六人民醫(yī)院骨科，上海市 200233 shzhangchunlin@

中圖分類號:R318 文獻(xiàn)標(biāo)識碼:A

文章編號: 1673-8225 (2010)24-04486-05

收稿日期：2010-01-16 修回日期：2010-02-09 (20090930003/GW·Z)

0 背景 由創(chuàng)傷、炎癥、腫瘤的外科治療所導(dǎo)致的骨缺損特別是長段骨缺損，是除了由骨折所致的骨不連外，，引起功能喪失并影響生活質(zhì)量的主要原因。目前傳統(tǒng)自體松質(zhì)骨移植是治療局部骨缺損的金標(biāo)準(zhǔn)，但是其存在不可否認(rèn)的局限性，如供骨區(qū)損傷、術(shù)后并發(fā)癥和治療費用等問題，更重要的是來源與數(shù)量的限制。異體

骨移植則不受形狀與數(shù)量的限制，可達(dá)到即時的骨量要求，其骨愈合過程與傳統(tǒng)的長段自體移植骨基本類似。但是異體骨經(jīng)各種滅菌處理，缺乏自身成骨作用，骨愈合過程緩慢且多存在不同程度的排斥反應(yīng)，因此效果欠佳。隨著生物材料學(xué)的興起，以生物材料來制作移植替代物的組織工程學(xué)正在不斷地發(fā)展，因此組織工程骨修復(fù)骨缺損也成為當(dāng)前的研究熱點。

組織工程學(xué)的基本原理是將種子細(xì)胞在體外培養(yǎng)擴(kuò)增后，接種于預(yù)先設(shè)計的負(fù)載生物

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  本文關(guān)鍵詞：組織工程骨修復(fù)骨缺損的研究進(jìn)展及臨床應(yīng)用，由筆耕文化傳播整理發(fā)布。