發(fā)布時(shí)間：2019-05-19 06:38

【摘要】：目的:觀察6-羥多巴(6-OHDA)損傷大鼠黑質(zhì)(SN)后下丘腦弓狀核(Arc)中酪氨酸羥化酶(TH)、離子鈣接頭蛋白1(Iba1)和阿片-促黑素皮質(zhì)素原(POMC)的表達(dá)變化,為帕金森病(PD)患者消瘦的發(fā)生機(jī)制提供線索。方法:20只SD大鼠隨機(jī)分為6-OHDA大鼠組(雙側(cè)SN內(nèi)注射6-OHDA)和對照組(注射等體積生理鹽水)。免疫熒光染色和Western blot檢測SN內(nèi)TH,以及Arc內(nèi)TH、Iba1和POMC的表達(dá)。結(jié)果:與對照組比較,6-OHDA大鼠組體重?zé)o明顯變化,但腹膜后脂肪重量從(7.550±0.670)g減少到(3.895±0.465)g(P0.01)。SN內(nèi)TH陽性細(xì)胞數(shù)量從56±5減少至14±2(P0.05);蛋白表達(dá)水平從0.75±0.11降低至0.41±0.09(P0.01)。Arc中TH陽性細(xì)胞數(shù)量從21±2減少到10±3(P0.05);Iba1陽性細(xì)胞數(shù)量從12±2增加到30±5(P0.05);POMC陽性細(xì)胞數(shù)量從16±4增加到31±2(P0.05)。下丘腦內(nèi)TH蛋白的表達(dá)水平從0.35±0.05降低到0.21±0.02(P0.01);Iba1蛋白的表達(dá)水平從0.23±0.06增加至0.51±0.04(P0.01);POMC的蛋白表達(dá)水平從0.37±0.05增加至0.65±0.03(P0.01)。結(jié)論:Arc中TH、Iba1和POMC表達(dá)變化可能與PD脂肪減少存在一定的聯(lián)系。
[Abstract]:Objective: to observe the expression of (TH), ion calcium junction protein 1 (Iba1) and opioid melanin-promoting proto-(POMC) in the arcuate nucleus of the hypothalamus after 6-hydroxydopa (6-OHDA) injury in the arcuate nucleus of the inferior colliculus (Arc) after 6-hydroxydopa (Arc) injury. It provides a clue to the mechanism of emaciation in patients with Parkinson's disease (PD). Methods: twenty SD rats were randomly divided into 6-OHDA group (bilateral SN injected with 6-OHDA) and control group (injected with saline of equal volume). The expression of TH, in SN and TH,Iba1 and POMC in Arc were detected by immunofluorescence staining and Western blot. Results: compared with the control group, the body weight of 6-OHDA rats had no significant change. However, the weight of retroperitoneal fat decreased from (7.550 鹵0.670) g to (3.895 鹵0.465) g (P 0.01), and the number of TH positive cells in SN decreased from 56 鹵5 to 14 鹵2 (P 0.05). The expression level of protein decreased from 0.75 鹵0.11 to 0.41 鹵0.09 (P01). The number of TH positive cells in Arc decreased from 21 鹵2 to 10 鹵3 (P 0.05), and the number of Iba1 positive cells increased from 12 鹵2 to 30 鹵5 (P 0.05). The number of POMC positive cells increased from 16 鹵4 to 31 鹵2 (P 0.05). The expression level of TH protein and Iba1 protein decreased from 0.35 鹵0.005 to 0.21 鹵0.02and 0.23 鹵0.06to 0.51 鹵0.04, respectively. The protein expression level of POMC increased from 0.37 鹵0.005 to 0.65 鹵0.003 (P 0.01). Conclusion: the expression of TH,Iba1 and POMC in Arc may be related to the decrease of PD fat.
【作者單位】： 新鄉(xiāng)醫(yī)學(xué)院河南省醫(yī)用組織再生重點(diǎn)實(shí)驗(yàn)室;東南大學(xué)附屬中大醫(yī)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.31200897) 新鄉(xiāng)醫(yī)學(xué)院博士科研基金(No.XYBSKYZZ201501) 河南省醫(yī)用組織再生重點(diǎn)實(shí)驗(yàn)室開放課題(No.KFKT16003)
【分類號】：R742.5

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