轉(zhuǎn)錄因子T-bet/GATA-3對哮喘小鼠Th1/Th2調(diào)節(jié)作用及滅活草分枝桿菌的干預(yù)研究
發(fā)布時間：2021-01-25 23:55
目的探討霧化吸入滅活草分枝桿菌對哮喘小鼠T-bet/GATA-3表達(dá)的影響,了解草分枝桿菌的免疫調(diào)節(jié)作用。方法將24只雄性Balb/c小鼠隨機(jī)分為對照組（A組）、哮喘組（B組）、治療組（C組）,每組8只。哮喘組和治療組于第0天、第7天和第14天用25μgOVA和1mg氫氧化鋁混合于PBS中的0.2ml混懸液腹腔注射小鼠,第21至27天用2%OVA·PBS霧化激發(fā)小鼠,每天30min,治療組在第28天至第32天霧化吸入1支滅活草分枝桿菌稀釋于10ml的生理鹽水中,每日1次。對照組則用生理鹽水代替腹腔注射和霧化激發(fā),哮喘組用生理鹽水代替滅活草分枝桿菌霧化治療,霧化過程中觀察小鼠的行為及活動情況等變化。最后一次霧化12h內(nèi)測小鼠氣道反應(yīng)性,24h內(nèi)將小鼠處死,支氣管肺泡灌洗液（BALF）,左肺組織病理切片行HE染色和PAS染色觀察小鼠肺內(nèi)炎癥改變,參照文獻(xiàn)方法行病理半定量評分,明確各組小鼠氣道炎癥嚴(yán)重程度;肺組織病理切片行免疫組化觀察T-bet和GATA-3的表達(dá)量;ELISA法檢測BALF中IFN-γ和IL-4表達(dá)水平;實時熒光定量PCR檢測肺組織中T-bet、GATA-3、STAT1、S...
【文章來源】：廣西醫(yī)科大學(xué)廣西壯族自治區(qū)
【文章頁數(shù)】：89 頁
【學(xué)位級別】：碩士
【部分圖文】：
對照組、哮喘組和治療組的肺組織Th1和Th2細(xì)胞流式細(xì)胞散點圖
圖 2-4 γδT 細(xì)胞分選純度及表達(dá) IFN-γ 及 IL-4 的分Figure2- 4.The purity of γ T cells after magnetic separation isolated from spleen of asthma group mice by using TCR γ the manufacture. γ T+CD3+cells were identified as γ T cellsγ T cells(A) before and (B) after magnetic separation.In sprecoatd with anti-mouse CD3 mAbs(5μg/ml;ebiosciencsupplemented with IL-2(10ng/ml;ebioscience).(C)A repOVA(100μg/ml;sigma),C representative γ T cells with OVAcells were harvested and stained for IFN-γ and IL-4.The expThe representative flow cytometric dot plots of IFN-γ+γ T cecells and IL-4+γ T cells in three group.All data were represmeans SD.The comparisons were determined by one-group;*p<0.05,compared with B group.討支氣管哮喘是一種慢性氣道疾病
本文編號：3000110
【文章來源】：廣西醫(yī)科大學(xué)廣西壯族自治區(qū)
【文章頁數(shù)】：89 頁
【學(xué)位級別】：碩士
【部分圖文】：
對照組、哮喘組和治療組的肺組織Th1和Th2細(xì)胞流式細(xì)胞散點圖
圖 2-4 γδT 細(xì)胞分選純度及表達(dá) IFN-γ 及 IL-4 的分Figure2- 4.The purity of γ T cells after magnetic separation isolated from spleen of asthma group mice by using TCR γ the manufacture. γ T+CD3+cells were identified as γ T cellsγ T cells(A) before and (B) after magnetic separation.In sprecoatd with anti-mouse CD3 mAbs(5μg/ml;ebiosciencsupplemented with IL-2(10ng/ml;ebioscience).(C)A repOVA(100μg/ml;sigma),C representative γ T cells with OVAcells were harvested and stained for IFN-γ and IL-4.The expThe representative flow cytometric dot plots of IFN-γ+γ T cecells and IL-4+γ T cells in three group.All data were represmeans SD.The comparisons were determined by one-group;*p<0.05,compared with B group.討支氣管哮喘是一種慢性氣道疾病
本文編號：3000110
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