目的:探討胰島素樣生長因子結(jié)合蛋白2（Insulinlike growth factor binding protein2,IGFBP2）對類風(fēng)濕關(guān)節(jié)炎成纖維樣滑膜細(xì)胞（RA-FLS）生物學(xué)行為的作用,以及IGFBP2對細(xì)胞外調(diào)節(jié)蛋白激酶（Extracellular regulated protein kinases1/2,ERK1/2）的作用。方法:首先,我們原代培養(yǎng)類風(fēng)濕關(guān)節(jié)炎成纖維樣滑膜細(xì)胞（RA-FLS）和正常成纖維樣滑膜細(xì)胞（FLS）,并采用q RT-PCR、Western blot及免疫熒光技術(shù)檢測IGFBP2 m RNA和蛋白在FLS和RA-FLS中的表達(dá)水平。其次,RA-FLS細(xì)胞被轉(zhuǎn)染si RNA-IGFBP2和si RNA-NC,q RT-PCR檢測IGFBP2在RA-FLS細(xì)胞中的干擾效果;CCK-8實(shí)驗(yàn)檢測IGFBP2敲低后對RA-FLS細(xì)胞增殖能力的影響;流式細(xì)胞儀檢測IGFBP2敲低后對RA-FLS細(xì)胞凋亡的影響;Transwell檢測IGFBP2敲低對RA-FLS細(xì)胞侵襲能力的影響;劃痕實(shí)驗(yàn)檢測IGFBP2敲低對RA-FLS細(xì)胞遷移能力的影響。此外,RA-... 

【文章來源】：暨南大學(xué)廣東省 211工程院校

【文章頁數(shù)】：78 頁

【學(xué)位級別】：博士

【部分圖文】：

qRT-PCR檢測IGFBP2mRNA在成纖維樣滑膜細(xì)胞（FLS）和類風(fēng)濕關(guān)節(jié)炎

3 Western blot檢測IGFBP2蛋白在成纖維樣滑膜細(xì)胞（FLS）和類風(fēng)濕關(guān)節(jié)炎維樣滑膜細(xì)胞(RA-FLS)中的表達(dá)水平。相對于FLS細(xì)胞，IGFBP2在RA-FLS中高表達(dá)。ure 3 Western blot assay was used to detect the expression level of IGFBP2 inoblast-like synoviosytes (FLS) and fibroblast-like synoviosytes from rheumatoidritis patients (RA-FLS). The expression level of IGFBP2 was high-expressed in-FLS cells compared with FLS cells.免疫熒光檢測 IGFBP2 在 RA-FLS 中表達(dá)分布為了進(jìn)一步確認(rèn) IGFBP2 在類風(fēng)濕關(guān)節(jié)炎成纖維樣滑膜細(xì)胞中表達(dá)，我們培養(yǎng)成纖維樣滑膜細(xì)胞（FLS）和類風(fēng)濕性關(guān)節(jié)炎成纖維樣滑膜細(xì)胞-FLS)，免疫熒光檢測 IGFBP2 蛋白在 RA-FLS 和 FLS 細(xì)胞中的表達(dá)分布情

FBP2 在類風(fēng)濕關(guān)節(jié)炎成纖維樣滑膜細(xì)胞中表達(dá)，主要分布在細(xì)-FLS 和 FLS 細(xì)胞，免疫熒光檢測 IGFBP2 蛋白在 RA-FLS 和情況。左側(cè)為細(xì)胞核 DAPI 著色，中間為 IGFBP2，右側(cè)為 M色，×200 倍）4 IGFBP2 was highly expressed in fibroblast-like synoviosoid arthritis patients (RA-FLS). We cultured RA-FLS and FLS cexpression level of IGFBP2 was detected by the immunofluorescesuperimposed images of IGFBP2 in green and nuclei (DAPInofluorescence staining, ×200）-PCR 檢測 IGFBP2 敲低后 mRNA 表達(dá)

【參考文獻(xiàn)】：
期刊論文
[1]Expression of insulin-like growth factor binding protein-2 in gastric carcinoma and its relationship with cell proliferation[J]. Liang-Hui Shi, Guo-Hai Zhao, Ya-Bin Xia, Yi-Sheng Zhang, Department of Surgery, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China Xiao-Qun Zhu, Department of Pathology, Wannan Medical College, Wuhu 241001, Anhui Province, China.  World Journal of Gastroenterology. 2006(39)



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