【摘要】：目的探討補(bǔ)體C3f去精氨酸片段(DRC3f)對于系統(tǒng)性硬化癥(SSc)小鼠皮膚纖維化模型構(gòu)建和轉(zhuǎn)化生長因子(TGF)-β1表達(dá)的影響。方法采用DRC3f聯(lián)合博來霉素皮下注射的方法構(gòu)建SSc小鼠模型,并用纖維染色和免疫組化方法比較聯(lián)合與不聯(lián)合DRC3f對SSc小鼠皮膚纖維化、皮膚增厚程度和TGF-β1表達(dá)的影響。結(jié)果聯(lián)合DRC3f和博來霉素注射組與單用博來霉素和博來霉素聯(lián)合無關(guān)多肽組比較,皮膚纖維化程度更加明顯,TGF-β1的表達(dá)也明顯增多(P0.05)。結(jié)論 DRC3f能夠促進(jìn)SSc模型小鼠皮膚纖維化和炎癥因子TGF-β1的表達(dá)。
[Abstract]:Objective to investigate the effect of complement C3f dearginine fragment (DRC3f) on the construction of skin fibrosis model and the expression of transforming growth factor (TGF)-尾 1 in (SSc) mice with systemic sclerosis. Methods SSc mouse model was established by DRC3f combined with bleomycin subcutaneous injection. The effects of DRC3f on skin fibrosis, skin thickening and TGF- 尾 1 expression in SSc mice were compared by fiber staining and immunohistochemistry. Results compared with the control group, the degree of skin fibrosis was more obvious and the expression of TGF- 尾 1 was significantly increased in the combined DRC3f and bleomycin injection group (P 0.05). Conclusion DRC3f can promote skin fibrosis and the expression of inflammatory factor TGF- 尾 1 in SSc model mice.
【作者單位】： 湖北民族學(xué)院風(fēng)濕性疾病發(fā)生與干預(yù)湖北省重點(diǎn)實(shí)驗(yàn)室;湖北民族學(xué)院附屬民大醫(yī)院病理科;湖北民族學(xué)院科技學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(81260458) 湖北省自然科學(xué)基金項(xiàng)目(2012FFA061)
【分類號】：R593.2;R-332

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