【摘要】：目的探討濕潤(rùn)燒傷膏(MEBO)干預(yù)糖尿病性潰瘍創(chuàng)面愈合的作用機(jī)制。方法于2015年6月選取145只12周齡的SPF級(jí)健康雄性SD大鼠,采用隨機(jī)數(shù)字表法將其分為空白組(35只)和糖尿病模型組(110只),糖尿病模型組采用腹腔注射鏈脲佐菌素(STZ)制備糖尿病大鼠模型,8周后選取空白組30只及糖尿病模型組成模大鼠90只,糖尿病模型組成模大鼠再采用隨機(jī)數(shù)字表法分為模型組、MEBO組及貝復(fù)濟(jì)組,每組30只,4組均制備潰瘍模型。成功后MEBO組予MEBO紗條、貝復(fù)濟(jì)組予重組牛堿性成纖維細(xì)胞生長(zhǎng)因子外用溶液浸透紗條、空白組及模型組予0.9%氯化鈉溶液紗條局部換藥處理,1次/d。分別于創(chuàng)面干預(yù)后第3、6、12天,每次隨機(jī)選取10只大鼠,觀察創(chuàng)面愈合速率;取相同位點(diǎn)創(chuàng)面組織,應(yīng)用酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)晚期糖基化終末產(chǎn)物(AGEs)、核因子κB(NF-κB)水平,熒光PCR技術(shù)檢測(cè)AGE受體(RAGE)mRNA表達(dá)水平,HE染色觀察創(chuàng)面組織炎性細(xì)胞浸潤(rùn)、成纖維細(xì)胞及血管生成情況。結(jié)果造模8周后,糖尿病模型組體質(zhì)量低于空白組,血糖水平高于空白組(P0.01)�？瞻捉M、模型組、MEBO組和貝復(fù)濟(jì)組第3、6、12天創(chuàng)面愈合速率比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01);其中模型組、MEBO組、貝復(fù)濟(jì)組創(chuàng)面愈合速率低于空白組,MEBO組、貝復(fù)濟(jì)組創(chuàng)面愈合速率高于模型組;第6、12天時(shí),貝復(fù)濟(jì)組創(chuàng)面愈合速率低于MEBO組(P0.05)�？瞻捉M、模型組、MEBO組和貝復(fù)濟(jì)組第3、6、12天創(chuàng)面組織AGEs水平比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.01);其中模型組、MEBO組、貝復(fù)濟(jì)組創(chuàng)面組織AGEs水平高于空白組,MEBO組、貝復(fù)濟(jì)組創(chuàng)面組織AGEs水平低于模型組(P0.05)�？瞻捉M、模型組、MEBO組和貝復(fù)濟(jì)組第3、6、12天創(chuàng)面組織NF-κB水平比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);其中第6、12天時(shí),模型組創(chuàng)面組織NF-κB水平高于空白組,MEBO組創(chuàng)面組織NF-κB水平低于模型組;第12天時(shí),貝復(fù)濟(jì)組創(chuàng)面組織NF-κB水平高于空白組、低于模型組(P0.05)。空白組、模型組、MEBO組、貝復(fù)濟(jì)組第3、6、12天創(chuàng)面組織RAGE mRNA表達(dá)水平比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);其中第6、12天時(shí),模型組、貝復(fù)濟(jì)組創(chuàng)面組織RAGE mRNA表達(dá)水平高于空白組,MEBO組創(chuàng)面組織RAGE mRNA表達(dá)水平低于模型組(P0.05)。病理結(jié)果顯示:干預(yù)后第12天,與模型組比較,MEBO組和貝復(fù)濟(jì)組能明顯促進(jìn)成纖維細(xì)胞及新生毛細(xì)血管生長(zhǎng),減少炎性細(xì)胞浸潤(rùn),其中MEBO組更為明顯。結(jié)論MEBO能明顯促進(jìn)糖尿病性潰瘍創(chuàng)面愈合,其機(jī)制可能與調(diào)控創(chuàng)面組織AGEs、NF-κB及RAGE mRNA的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the mechanism of MEBO (MEBO) on wound healing of diabetic ulcer. Methods in June 2015, 145 SPF grade healthy male SD rats aged 12 weeks were selected and randomly divided into blank group (35 rats) and diabetic model group (110 rats). Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ). After 8 weeks, 30 rats in the blank group and 90 rats in the diabetic model were selected. The diabetic model rats were randomly divided into three groups: model group, MEBO group and Befuji group with 30 rats in each group. The ulcer models were made in 4 groups. After success, MEBO group was given MEBO gauze, Befuji group was soaked with recombinant bovine basic fibroblast growth factor solution, blank group and model group were treated with 0.9% sodium chloride solution gauze, once a day. Ten rats were randomly selected at the 3rd day after the intervention to observe the wound healing rate. The level of (AGEs), nuclear factor 魏 B (NF- 魏 B) and the expression of AGE receptor (RAGE) mRNA were detected by enzyme linked immunosorbent assay (ELISA) and fluorescence PCR technique. The infiltration of inflammatory cells, fibroblasts and angiogenesis were observed by HE staining. Results after 8 weeks, the body mass of diabetic model group was lower than that of blank group, and the blood glucose level was higher than that of blank group (P0.01). The rate of wound healing in blank group, model group, MEBO group and Befuji group was significantly higher than that in control group (P0.01). The wound healing rate of model group, MEBO group and befuji group was lower than that of blank group. The wound healing rate of MEBO group and Befuji group was higher than that of model group, and the wound healing rate of Befuji group was lower than that of MEBO group at the 6th day (P0.05). There were significant differences in AGEs levels between the blank group, model group, MEBO group and Befuji group on the 12th day (P0.01). The level of AGEs in model group, MEBO group and Befuji group was higher than that in blank group, and the AGEs level in MEBO group and Befuji group was lower than that in model group (P0.05). There were significant differences in NF- 魏 B levels between the blank group, model group, MEBO group and Befuji group on the 12th day (P0.05). On the 6th day, the level of NF- 魏 B in the model group was higher than that in the blank group, the level of NF- 魏 B in the MEBO group was lower than that in the model group, and on the 12th day, the NF- 魏 B level in the wound tissue in the befuji group was higher than that in the blank group and lower than that in the model group (P0.05). In blank group, model group, MEBO group and Befuji group, the expression of RAGE mRNA in wound tissue was significantly higher than that in control group (P0.05). At the 6th day, the expression of RAGE mRNA in the model group and Befuji group was higher than that in the blank group, and the expression level of RAGE mRNA in the MEBO group was lower than that in the model group (P0.05). The pathological results showed that on the 12th day after intervention, MEBO group and Befuji group could significantly promote fibroblast and neovascularization growth, and reduce inflammatory cell infiltration, especially in MEBO group, compared with the model group. Conclusion MEBO can obviously promote wound healing of diabetic ulcer, and its mechanism may be related to regulating the expression of AGEs,NF- 魏 B and RAGE mRNA in wound tissue.
【作者單位】： 廣西中醫(yī)藥大學(xué)第一附屬醫(yī)院;廣西壯族自治區(qū)中醫(yī)藥研究院;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81302975)
【分類(lèi)號(hào)】：R587.2

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