【摘要】：目的觀察辛伐他汀與硫酸鈣混合后對骨質(zhì)疏松骨缺損修復(fù)的促進(jìn)作用。方法選用健康3月齡雌性SD大鼠48只,切除雙側(cè)卵巢,低鈣飲食3月,并將脛骨上端造成3 mm骨缺損,隨機(jī)均分為3組,未植骨組直接縫合皮膚,硫酸鈣組在骨缺損處植入硫酸鈣,載辛伐他汀硫酸鈣組植入載辛伐他汀硫酸鈣,植入后4、8周取標(biāo)本進(jìn)行組織學(xué)觀察骨痂形成情況,植入后8周行Micro-CT檢測,于12周對標(biāo)本進(jìn)行生物力學(xué)分析。結(jié)果植入4、8周時,載辛伐他汀硫酸鈣組骨愈合程度較硫酸鈣組和未植骨組有顯著性改善,8周時,Micro-CT檢測骨缺損區(qū)內(nèi)骨體積分?jǐn)?shù)(BV/TV),組織骨密度(t BMD),骨小梁數(shù)量(Tb.N),載辛伐他汀硫酸鈣組優(yōu)于后兩組,12周時生物力學(xué)檢測,載辛伐他汀硫酸鈣組失效強度和剛度優(yōu)于硫酸鈣組和未植骨組(P0.05)。結(jié)論載辛伐他汀硫酸鈣對骨質(zhì)疏松性骨缺損具有良好的成骨和骨誘導(dǎo)作用,可以較好地促進(jìn)骨缺損的修復(fù)。
[Abstract]:Objective to observe the promoting effect of simvastatin and calcium sulfate on the repair of osteoporosis bone defect. Methods Forty-eight healthy 3-month-old female SD rats were randomly divided into 3 groups, with bilateral ovaries resected and low-calcium diet for 3 months, and 3 mm bone defects were caused at the upper end of the tibia, and the skin was sutured directly in the ungrafted group. Calcium sulfate was implanted into bone defect in calcium sulfate group, and calcium sulfate was implanted in simvastatin sulfate group. Histological observation of callus formation was performed 8 weeks after implantation, and Micro-CT was detected 8 weeks after implantation. Biomechanical analysis of the specimens was carried out at 12 weeks. Results at the 8th week of implantation, the healing degree of bone in the calcium sulfate group was significantly improved than that in the calcium sulfate group and the ungrafted group. At 8 weeks, bone volume fraction (BV/TV) and bone density (t BMD),) in the bone defect area were measured by Micro-CT. The number of trabeculae (Tb.N) and the number of trabeculae (Tb.N) in the calcium sulfate group were better than those in the latter two groups. At 12 weeks, the failure strength and stiffness of the calcium sulfate group were better than those of the calcium sulfate group and the ungrafted group (P0.05). Conclusion calcium simvastatin sulfate has good osteogenic and bone-inducing effects on osteoporotic bone defect and can promote the repair of bone defect.
【作者單位】： 南京醫(yī)科大學(xué)附屬蘇州醫(yī)院關(guān)節(jié)外科;蘇州大學(xué)附屬第二醫(yī)院關(guān)節(jié)外科;
【基金】：蘇州市科教興衛(wèi)發(fā)展項目(KJXW2013024)
【分類號】：R580;R683
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本文編號：2414836