【摘要】：目的通過觀察狹鱈魚皮膠原多肽對去勢大鼠骨質(zhì)疏松模型骨微結(jié)構(gòu)的影響,探討其防治骨質(zhì)疏松的可行性。方法成年Wistar雌性大鼠60只,體質(zhì)量(250±10)g,隨機(jī)分為5組(n=12),分別為正常對照組(A組)、骨質(zhì)疏松模型組(B組)、骨質(zhì)疏松模型+狹鱈魚皮膠原多肽預(yù)防組(C組)、骨質(zhì)疏松模型+狹鱈魚皮膠原多肽低劑量治療組(D組)、骨質(zhì)疏松模型+狹鱈魚皮膠原多肽高劑量治療組(E組),每組12只。B、C、D、E組采用摘除雙側(cè)卵巢法制備大鼠骨質(zhì)疏松模型。C組從術(shù)前4周開始、D、E組從術(shù)后6周開始,每天按照1.0、0.5、1.0 g/kg行狹鱈魚皮膠原多肽灌胃,連續(xù)6周;A、B組于術(shù)后同時間點(diǎn)給予等體積生理鹽水灌胃。末次給藥24 h后,A、B組大鼠攝股骨X線片并測定灰度值;然后頸椎脫臼法處死各組大鼠,取左側(cè)脛骨近端骨組織行HE染色,觀察骨組織病理學(xué)改變,測量骨小梁數(shù)量(trabecular number,TN)、平均骨小梁厚度(mean trabecular plate thickness,MTPT)、平均骨小梁間距(mean trabecular plate spacing,MTPS)、骨小梁體積百分比(trabecular bone volume,TBV)、平均骨皮質(zhì)厚度(mean bone cortical thickness,MBCT);免疫組織化學(xué)染色觀測降鈣素受體(caltitonin receptor,CTR)及IL-1表達(dá)水平。結(jié)果 B組大鼠股骨灰度值顯著低于A組(t=45.130,P=0.000),表明去勢大鼠骨質(zhì)疏松模型制備成功。組織學(xué)觀察示,A、C、E組TN、MTPS、TBV、MBCT與B組比較,差異有統(tǒng)計學(xué)意義(P0.05);C組各骨組織形態(tài)計量學(xué)參數(shù)與D、E組比較,差異均有統(tǒng)計學(xué)意義(P0.05);D組TN、MTPS、TBV、MBCT與A組比較差異有統(tǒng)計學(xué)意義(P0.05);E組僅MTPS與A組比較差異有統(tǒng)計學(xué)意義(P0.05);E組MTPS、TBV、MBCT與D組比較,差異有統(tǒng)計學(xué)意義(P0.05)。免疫組織化學(xué)染色觀察示,A、C、D、E組CTR、IL-1表達(dá)水平較B組降低,C、E組低于D組,差異有統(tǒng)計學(xué)意義(P0.05);其余組間比較差異均無統(tǒng)計學(xué)意義(P0.05)。結(jié)論狹鱈魚皮膠原多肽能夠改善骨質(zhì)疏松大鼠的骨微結(jié)構(gòu),其機(jī)制可能與抑制骨組織中CTR、IL-1表達(dá)有關(guān),但尚未發(fā)現(xiàn)其對骨質(zhì)疏松癥有預(yù)防作用。
[Abstract]:Objective to observe the effect of collagen peptide from cod skin on bone microstructure of ovariectomized rats and to explore the feasibility of prevention and treatment of osteoporosis. Methods Sixty adult Wistar female rats, weighing (250 鹵10) g, were randomly divided into 5 groups (n = 12): normal control group (group A) and osteoporosis model group (group B). Osteoporosis model cod skin collagen peptide prevention group (C group), osteoporosis model cod skin collagen peptide low dose treatment group (D group), osteoporosis model narrow cod skin collagen peptide high dose treatment group (E group), osteoporosis model cod skin collagen peptide prevention group (group C), osteoporosis model cod skin collagen peptide low dose treatment group (group D), Osteoporosis model was established in each group with 12 rats. The osteoporosis model was established in group C from 4 weeks before operation and from 6 weeks after operation in group D. Collagen polypeptide from cod skin was perfused daily for 6 weeks according to 1.0 g/kg. Group B was given the same volume of normal saline at the same time after operation. Twenty-four hours after the last administration, the rats in group A and B took X-ray films of femur and measured the gray value. Then the rats were killed by cervical dislocated method. The left proximal tibia bone tissue was taken for HE staining. The histopathological changes of bone were observed. The number of trabecular bone (trabecular number,TN) and the mean trabecular thickness (mean trabecular plate thickness,MTPT) were measured. Mean trabecular spacing (mean trabecular plate spacing,MTPS), trabecular volume percentage (trabecular bone volume,TBV), and mean cortical thickness (mean bone cortical thickness,MBCT); The expression of calcitonin receptor (caltitonin receptor,CTR) and IL-1 were detected by immunohistochemical staining. Results the gray value of femur in group B was significantly lower than that in group A (t = 45.130), which indicated that the model of osteoporosis in ovariectomized rats was successfully prepared. Histological observation showed that there was significant difference in TN,MTPS,TBV,MBCT between group A and group B (P0.05). The bone histomorphometry parameters in); C group were significantly different from those in group E (P0.05). The difference of TN,MTPS,TBV,MBCT between group D and group A was statistically significant (P0.05). Only MTPS in group); E was significantly different from group A (P0.05). MTPS,TBV,MBCT in group E was significantly different from that in group D (P0.05). Immunohistochemical staining showed that the expression of CTR,IL-1 in group A was lower than that in group B, and that in group C was lower than that in group D (P0.05). There was no significant difference between the other groups (P0.05). Conclusion the collagen peptide from cod skin can improve the bone microstructure of osteoporosis rats, and its mechanism may be related to the inhibition of CTR,IL-1 expression in bone tissue, but it has not been found to have a preventive effect on osteoporosis.
【作者單位】： 青島大學(xué)醫(yī)學(xué)部中西醫(yī)結(jié)合中心;青島大學(xué)醫(yī)學(xué)部解剖學(xué)教研室;青島大學(xué)醫(yī)學(xué)部臨床醫(yī)學(xué)院;青島市海慈醫(yī)療集團(tuán)關(guān)節(jié)外科;青島大學(xué)藥學(xué)院;
【分類號】：R580
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