【摘要】：目的通過流式細(xì)胞技術(shù)檢測外周血γδT細(xì)胞Vδ2亞群的比例和功能表型,研究原發(fā)性干燥綜合征(primary Sjogren's syndrome,PSS)患者γδT細(xì)胞Vδ2細(xì)胞亞群數(shù)量及功能變化,及其與疾病活動程度的相關(guān)性。方法研究對象分為PSS患者27例,疾病對照組原發(fā)性膽汁性肝硬化(primary biliary cirrhosis,PBC)患者10例,健康對照(heal thy control,HC)15例,分別通過流式細(xì)胞技術(shù)檢測其外周血γδT細(xì)胞Vδ2亞群占CD3+淋巴細(xì)胞和γδT細(xì)胞的比例,并檢測Vδ2細(xì)胞活化、殺傷功能、細(xì)胞增殖、細(xì)胞凋亡相關(guān)功能表型,比較疾病組和對照組之間各指標(biāo)是否存在顯著差異。最后,結(jié)合患者臨床資料對PSS患者通過疾病活動評估指數(shù)(ESSDAI)進(jìn)行評分,研究V82細(xì)胞比例與疾病活動程度的相關(guān)性。結(jié)果PSS患者組、DC組、HC組Vδ2細(xì)胞占CD3+淋巴細(xì)胞比例分別為2.152±0.292%,3.790±0.494%,4.900±0.811%,PSS患者組較HC組、DC組均顯著降低(P值分別為0.0005,0.0117),而DC組較HC組無明顯變化(P值為0.3244)。PSS患者組、DC組、HC組Vδ2細(xì)胞占γδT細(xì)胞比例分別為60.38±3.930%,75.79±4.717%,78.75±3.719%,PSS患者組較HC組和DC組顯著降低(P值為0.0045,0.0199),而DC組較HC組無明顯變化(P值為0.6279)。Vδ2細(xì)胞表達(dá)CD27、CD38、CD69、CD62L、CD45RA、CD45RO、HLA-DR、腫瘤壞死因子-α(TNF-α)、干擾素-r(IFN-r)、顆粒酶(Granzyme A/B)和穿孔素(perforin)、Ki-67、7-AAD等細(xì)胞活化、功能、增殖相關(guān)分子,且與健康對照組無明顯差異。而PSS患者組凋亡相關(guān)分子Annexin表達(dá)顯著增加。PSS患者外周血淋巴細(xì)胞中Vδ2細(xì)胞所占比例與基于ESSDAI評分的疾病活動程度呈線性負(fù)相關(guān)。結(jié)論與HC和DC組相比,PSS患者外周血Vδ2細(xì)胞占CD3+淋巴細(xì)胞和γδT細(xì)胞的比例明顯下降。Vδ2細(xì)胞占CD3+淋巴細(xì)胞比例與基于ESSDAI評分的疾病活動程度呈線性負(fù)相關(guān)。HC組相比,PSS患者Vδ2細(xì)胞除凋亡相關(guān)分子Annexin Ⅴ表達(dá)顯著增加外,其他細(xì)胞功能表型表達(dá)無明顯變化。
[Abstract]:Objective to study the number and function of V 未 2 subsets of 緯 未 T cells in patients with primary Sjogren's syndrome (primary Sjogren's syndrome,PSS) by detecting the proportion and function of V 未 2 subsets of 緯 未 T cells by flow cytometry. And its correlation with the degree of disease activity. Methods the subjects were divided into three groups: 27 patients with PSS, 10 patients with primary biliary cirrhosis (primary biliary cirrhosis,PBC) and 15 healthy controls (heal thy control,HC). The ratio of V 未 2 subsets of 緯 未 T cells to CD3 lymphocytes and 緯 未 T cells was detected by flow cytometry, and the activation, killing function, cell proliferation and apoptosis related functional epitopes of V 未 2 cells were detected. To compare whether there were significant differences between the disease group and the control group. Finally, the relationship between the proportion of V82 cells and the degree of disease activity was studied by scoring the disease activity assessment index (ESSDAI) in patients with PSS. Results the ratio of V 未 2 cells to CD3 lymphocytes in PSS group, DC group and HC group were 2.152 鹵0.292and 3.790 鹵0.4944.900 鹵0.8114g, respectively. The percentage of V 未 2 cells in DC group was significantly lower than that in HC group (P = 0.0005, 0.0117, respectively). The ratio of V 未 2 cells to 緯 未 T cells in DC group, DC group and DC group was 60.38 鹵3.930 and 75.79 鹵4.717 78.75 鹵3.719, respectively. The expression of CD27,CD38,CD69,CD62L,CD45RA,CD45RO,HLA-DR, in PSS group was significantly lower than that in HC group and DC group (P = 0.0045,0.0199), but there was no significant change in DC group compared with HC group (P = 0.6279). V 未 2 cell expression). Tumor necrosis factor- 偽 (TNF- 偽), interferon- r (IFN-r), granzyme (Granzyme A / B) and perforin (perforin), Ki-67,7-AAD were activated, functionally and proliferation-related molecules were not significantly different from those in healthy control group. The proportion of V 未 2 cells in peripheral blood lymphocytes of PSS patients was negatively correlated with the degree of disease activity based on ESSDAI score. Conclusion compared with HC and DC groups, the percentage of V 未 2 cells in CD3 lymphocytes and 緯 未 T cells in peripheral blood of PSS patients was significantly decreased, and the proportion of V 未 2 cells to CD3 lymphocytes was linearly negatively correlated with the disease activity based on ESSDAI score. Compared with HC group, the percentage of V 未 2 cells in CD3 lymphocytes and 緯 未 T cells in PSS patients was significantly decreased. In patients with PSS, the expression of Annexin 鈪，

本文編號：2371922