【摘要】：目的觀察不同病程糖尿病大鼠下頜下腺內(nèi)神經(jīng)元型一氧化氮合酶(nN OS)和神經(jīng)營養(yǎng)因子-3(NT-3)的表達變化,探討其對糖尿病下頜下腺結(jié)構(gòu)和功能的影響。方法 48只SD大鼠隨機分為對照組、糖尿病組和治療組。糖尿病組和治療組給予腹腔注射鏈脲佐菌素復(fù)制2型糖尿病模型,治療組給予胰島素治療,于造模后3個月和6個月每組分別處死8只大鼠,測定空腹血糖,并取下頜下腺組織,分別進行HE染色、免疫組織化學染色和計算機圖像分析。結(jié)果糖尿病組血糖較同期對照組升高且隨著病程的延長顯著,治療組血糖較同期糖尿病組下降。光鏡下,與對照組相比,糖尿病組下頜下腺腺泡萎縮加重,顆粒曲管數(shù)目減少,管腔變窄,治療組無明顯變化;免疫組織化學法檢測顯示,nNOS在糖尿病組表達較同期對照組增強,在造模3個月表達最強,治療組表達較同期糖尿病組降低;NT-3在糖尿病組表達較同期對照組降低且隨病程的延長減少,治療組較同期糖尿病組增強。結(jié)論糖尿病大鼠下頜下腺內(nèi)nNOS表達升高和NT-3表達下降,可能是引起糖尿病下頜下腺結(jié)構(gòu)和功能紊亂的重要原因;胰島素治療可能與nNOS和NT-3的表達改變有關(guān)。
[Abstract]:Objective to observe the expression of neuronal nitric oxide synthase (nN OS) and neurotrophic factor 3 (NT-3) in the submandibular gland of diabetic rats with different course of diabetes and to explore its effect on the structure and function of the submandibular gland of diabetic rats. Methods 48 SD rats were randomly divided into control group, diabetes group and treatment group. Type 2 diabetic model was induced by intraperitoneal injection of streptozotocin in diabetic group and treatment group, and insulin was given in treatment group. Eight rats were killed in each group at 3 months and 6 months after model, and fasting blood glucose was measured. The submandibular gland was stained with HE, immunohistochemistry and computer image analysis. Results the blood glucose of the diabetic group was higher than that of the control group, and the blood glucose of the treatment group was lower than that of the diabetic group. Under light microscope, compared with the control group, the submandibular acinar atrophy was aggravated, the number of granular convoluted tubes decreased and the lumen became narrower in the diabetic group, but there was no obvious change in the treatment group. Immunohistochemical method showed that the expression of nNOS in the diabetic group was higher than that in the control group, and the expression in the treatment group was lower than that in the diabetic group. The expression of NT-3 in the diabetic group was lower than that in the control group and decreased with the prolongation of the course of disease. Conclusion the increase of nNOS expression and the decrease of NT-3 expression in the submandibular glands of diabetic rats may be the important causes of the structural and functional disorders of the submandibular glands in diabetic rats, and insulin therapy may be related to the changes of nNOS and NT-3 expression.
【作者單位】： 安徽醫(yī)科大學基礎(chǔ)醫(yī)學院;安徽醫(yī)科大學臨床醫(yī)學院;安徽人口職業(yè)學院解剖學與組織胚胎學教研室;
【基金】：安徽省高校省級自然科學研究項目(編號:KJ2012A164)
【分類號】：R587.2

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