【摘要】：目的建立尿中錫的原子熒光分光光度測(cè)定方法。方法取2.50 m L尿樣加入1.50 m L濃硝酸后,用石墨消解儀消化,以體積分?jǐn)?shù)為4.00%的鹽酸溶液定容至10.00 m L,加入質(zhì)量濃度為100 g/L的硫脲-抗壞血酸2.50 m L,以體積分?jǐn)?shù)為4.00%的鹽酸溶液定容至25.00 m L(相當(dāng)于稀釋尿樣10倍)后,取1.00 m L樣品采用原子熒光光度法進(jìn)行測(cè)定。結(jié)果尿中錫在質(zhì)量濃度為4.00~200.00μg/L范圍內(nèi)線性關(guān)系良好,相關(guān)系數(shù)為0.999 5;方法檢出限為0.20μg/L,加標(biāo)回收率為100.20%~100.84%,批內(nèi)相對(duì)標(biāo)準(zhǔn)偏差(RSD)為0.11%~2.01%,批間RSD為1.37%~5.58%;樣品在4℃條件下可保存7 d。結(jié)論本方法靈敏度高,準(zhǔn)確性好,操作簡(jiǎn)便,適用于人尿中錫的日常監(jiān)測(cè)。
[Abstract]:Objective to establish an atomic fluorescence spectrophotometric method for the determination of tin in urine. Methods 2.50 mL urine samples were digested by graphite digester after 1.50 mL concentrated nitric acid was added. When the volume fraction of hydrochloric acid was 4.00%, the volume of hydrochloric acid solution was 10.00 mL, the concentration of thiourea-ascorbic acid was 2.50 mL, and the volume fraction of hydrochloric acid solution was 4.00% to 25.00 m L (was 10 times that of dilute urine. 1.00 mL sample was determined by atomic fluorescence spectrophotometry. Results the calibration curve of tin in urine was linear in the range of 4.00 ~ 200.00 渭 g / L, the correlation coefficient was 0.999, the detection limit was 0.20 渭 g / L, the recovery rate was 100.20g / L, the relative standard deviation (RSD) was 0.112.01, the interassay RSD was 1.375.58. the sample could be preserved at 4 鈩，

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