【摘要】：目的探討糖尿病合并去勢(shì)大鼠骨微結(jié)構(gòu)的改變。方法 6月齡雌性大鼠40只,隨機(jī)分為對(duì)照組、假手術(shù)組、去勢(shì)組、糖尿病組和糖尿病合并去勢(shì)組。6 w和15 w后應(yīng)用Micro-CT測(cè)定股骨檢測(cè)骨密度和骨微結(jié)構(gòu);利用Van Gieson膠原纖維染色法觀察膠原纖維沉積變化;HE染色分析大鼠股骨頭骨小梁厚度(Tb.Th)和骨小梁間距(Tb.Sp)變化。結(jié)果與對(duì)照組相比,去勢(shì)6 w和15 w組骨β-膠原降解產(chǎn)物水平和Tb.Sp明顯升高,骨密度值和Tb.Th明顯下降。去勢(shì)15 w組骨膠原纖維明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。糖尿病組15 w松質(zhì)骨β-膠原降解產(chǎn)物水平、膠原纖維和Tb.SP明顯升高,骨密度值、膠原纖維和Tb.Th明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。糖尿病合并去勢(shì)組6 w和15 w組松質(zhì)骨β-膠原降解產(chǎn)物水平、膠原纖維和Tb.Sp明顯升高,骨密度值和Tb.Th明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論糖尿病合并去勢(shì)早期是以去勢(shì)為主導(dǎo)致的骨質(zhì)疏松,后期則由糖尿病緩慢的骨分解加持,骨質(zhì)疏松繼續(xù)加重。
[Abstract]:Objective to investigate the changes of bone microstructure in diabetic castrated rats. Methods Forty 6-month-old female rats were randomly divided into control group, sham operation group, castration group, diabetes mellitus group and diabetic combined castration group. Bone mineral density (BMD) and bone microstructure were measured by Micro-CT after 6 weeks and 15 weeks. The changes of collagen deposition were observed by Van Gieson staining. The changes of trabecular thickness (Tb.Th) and trabecular spacing (Tb.Sp) of femoral head in rats were analyzed by HE staining. Results compared with the control group, bone 尾 -collagen degradation products and Tb.Sp were significantly increased, bone mineral density (BMD) and Tb.Th were significantly decreased in ovariectomized groups at 6 and 15 weeks. In castration group, collagen fibers decreased significantly at 15 weeks (P0.05). In the diabetic group, 尾 -collagen degradation product level, collagen fiber and Tb.SP increased significantly, bone mineral density, collagen fiber and Tb.Th decreased significantly in the diabetic group at 15 weeks, the difference was statistically significant (P0.05). The levels of 尾 -collagen degradation products, collagen fibers and Tb.Sp, bone mineral density (BMD) and Tb.Th decreased significantly in castrated diabetic group at 6 and 15 weeks (P0.05). Conclusion Osteoporosis is the main cause of Ovariectomization in the early stage of diabetes mellitus, and osteoporosis is aggravated in the later stage by the slow osteolysis of diabetes mellitus.
【作者單位】： 福建莆田學(xué)院臨床醫(yī)學(xué)院;
【分類號(hào)】：R580;R587.2

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