【摘要】：目的研究間歇性斷食對老年前期肥胖大鼠代謝和腸道菌群的影響。方法雌性Wistar大鼠經(jīng)42周高脂高糖飼料飼養(yǎng)造模,根據(jù)體質(zhì)量選取模型鼠進行間歇性斷食干預。干預方法為每2周斷食72 h,總干預時間18周。干預后進行口服葡萄糖耐量試驗、血脂4項檢測。收集糞便,通過Illumina高通量測序檢測16S r RNA基因V4可變區(qū),運用QIIME及LEf Se分析腸道菌群。結果間歇性斷食組體質(zhì)量相對于模型對照組顯著下降(P0.01);高密度脂蛋白膽固醇和低密度脂蛋白膽固醇均顯著下降(P0.05);空腹血糖顯著上升(P0.01);葡萄糖耐量測試曲線下面積顯著高于模型對照組,糖耐量減退(P0.05);HE染色顯示間歇性斷食輕度減少肝臟脂肪變性。腸道菌群結果顯示,斷食組腸道菌群得到顯著改善,具體表現(xiàn)為YS2、RF32、Helicobacteraceae(螺桿菌科)增加,Lactobacillus(乳桿菌屬)、Roseburia(羅氏菌屬)、Erysipelotrichaceae(韋榮球菌科)、Ralstonia(青枯菌屬)、Bradyrhizobiaceae(慢生根瘤菌科)和RF39減少。Spearman相關性分析發(fā)現(xiàn)Bradyrhizobiaceae與總膽固醇、高密度脂蛋白膽固醇呈正相關;大鼠體質(zhì)量與RF39呈負相關。結論間歇性斷食能改善腸道菌群,降低老年前期肥胖大鼠體質(zhì)量和血脂水平,但對糖代謝有不良影響。
[Abstract]:Objective to study the effects of intermittent food deprivation on metabolism and intestinal flora of obese rats. Methods female Wistar rats were fed with high fat and high sugar forage for 42 weeks. The intervention method was 72 hours every 2 weeks, and the total intervention time was 18 weeks. After intervention, oral glucose tolerance test and 4 items of blood lipids were tested. Feces were collected and the V4 variable region of 16s r RNA gene was detected by Illumina high-throughput sequencing. The intestinal flora was analyzed by QIIME and LEf Se. Results compared with the model control group, the body mass of intermittent diet group decreased significantly (P0.01), the high density lipoprotein cholesterol and low density lipoprotein cholesterol decreased significantly (P0.05), the fasting blood glucose increased significantly (P0.01), and the area under the curve of glucose tolerance test was significantly increased. Significantly higher than the model control group, Impaired glucose tolerance (P0.05) and HE staining showed that intermittent food deprivation slightly reduced hepatic steatosis. The results of intestinal microflora showed that the intestinal flora of the diet group was significantly improved. YS2,RF32,Helicobacteraceae (Helicobacteriaceae) increased Lactobacillus (Lactobacillus) Roseburia (Roxburia) Erysipelotrichaceae (Veronella) and Bradyrhizobiaceae (Bradyrhizobiaceae) and RF39 decreased. Spearman correlation analysis showed that Bradyrhizobiaceae was positively correlated with total cholesterol and high density lipoprotein cholesterol. There was a negative correlation between body mass and RF39 in rats. Conclusion intermittent feeding can improve intestinal flora, decrease body mass and blood lipid level of obese rats, but have a negative effect on glucose metabolism.
【作者單位】： 南方醫(yī)科大學公共衛(wèi)生學院環(huán)境衛(wèi)生學系;南方醫(yī)科大學珠江醫(yī)院檢驗醫(yī)學部;南方醫(yī)科大學南方醫(yī)院神經(jīng)內(nèi)科;
【基金】：國家自然科學基金優(yōu)秀青年基金(31322003)~~
【分類號】：R589.2

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