【摘要】：目的應(yīng)用艾滋病病毒(HIV)核酸檢測(cè)技術(shù),對(duì)男男性行為者(MSM)中HIV初篩檢測(cè)陽(yáng)性,蛋白印跡試驗(yàn)(WB)確證結(jié)果為不確定者和HIV初篩檢測(cè)陰性者進(jìn)行篩查,及時(shí)診斷MSM中的HIV急性期感染者,盡快啟動(dòng)抗病毒治療,探索我國(guó)MSM中HIV急性期感染篩查診斷和治療的工作模式。方法 2015年7月至2016年6月,通過(guò)方便樣本的招募方式,選取天津地區(qū)的MSM 2 000人,同時(shí)使用艾滋病第三代和第四代快速檢測(cè)試劑進(jìn)行平行檢測(cè),陰性樣本統(tǒng)一進(jìn)行集合核酸(HIV Pooled PCR)檢測(cè),對(duì)酶聯(lián)免疫吸附試驗(yàn)(ELISA)結(jié)果為陰性、WB檢測(cè)結(jié)果為陰性和不確定者接受擴(kuò)增核酸檢測(cè)(NAAT),結(jié)果為陽(yáng)性者且檢測(cè)值≤1 000拷貝/mL,進(jìn)行1-2周隨訪,并在隨后接受核酸和WB檢測(cè),陽(yáng)性檢測(cè)者檢測(cè)值1 000拷貝/mL,則重復(fù)擴(kuò)增核酸檢測(cè)步驟,并最終按照檢測(cè)結(jié)果判定HIV急性期感染者。結(jié)果共招募符合條件的調(diào)查對(duì)象1 914人,第三代和第四代快速檢測(cè)試劑同時(shí)檢測(cè)出陽(yáng)性73人。4例WB不確定結(jié)果樣本經(jīng)過(guò)兩次NAAT檢測(cè)被診斷為HIV感染。通過(guò)集合核酸檢測(cè)發(fā)現(xiàn)3例結(jié)果為陽(yáng)性的樣本。共6例HIV急性期樣本接受抗病毒治療,6例接受治療樣本的病毒載量分別降至46拷貝/mL、230拷貝/mL、72拷貝/mL、20拷貝/mL、622拷貝/mL和48拷貝/mL。結(jié)論使用第三代快速檢測(cè)試劑開(kāi)展HIV急性期檢測(cè)工作仍具有較大實(shí)際意義和可行性,同時(shí)在日常HIV檢測(cè)工作中應(yīng)充分重視和發(fā)揮核酸檢測(cè),減少M(fèi)SM在HIV檢測(cè)過(guò)程中,從其WB確證結(jié)果不確定到隨訪至其WB檢測(cè)結(jié)果陽(yáng)性中間傳播HIV的風(fēng)險(xiǎn)。
[Abstract]:Objective to detect HIV positive in (MSM) of men who have sex with men by (HIV) nucleic acid detection technique, and to screen those who are uncertain and negative by Western blot test (WB). To diagnose the acute infection of HIV in MSM, to start the antiviral therapy as soon as possible, and to explore the mode of screening, diagnosis and treatment of HIV infection in MSM in our country. Methods from July 2015 to June 2016, 2 000 MSM samples were recruited from Tianjin area, and the third and fourth generation rapid detection reagents were used for parallel detection. Negative samples were uniformly tested for (HIV Pooled PCR) of aggregate nucleic acids. For those whose (ELISA) results were negative and uncertain, those with positive (NAAT), results and detection value 鈮，

本文編號(hào)：2210285