【摘要】：目的觀察去卵巢骨質(zhì)疏松大鼠頜骨及股骨來源的骨髓間充質(zhì)干細胞增殖及成骨分化能力的差異。方法雌性SD大鼠雙側(cè)卵巢切除建立骨質(zhì)疏松模型。培養(yǎng)正常和骨質(zhì)疏松狀態(tài)下大鼠頜骨及股骨來源的骨髓間充質(zhì)干細胞。觀察細胞形態(tài),進行MTT和CCK-8實驗比較四組干細胞的增殖能力。制備四組干細胞膜片與TCP復合進行大網(wǎng)膜移植,術(shù)后6w行組織學觀察。結(jié)果去卵巢組BMSCs在同等時間條件下與未去卵巢組比較,初期增殖速度無顯著性差異,3 d后增殖速度則慢于健康組(P0.05)。頜骨和股骨BMSCs在健康骨質(zhì)環(huán)境下,細胞的OD值各時間段均未出現(xiàn)統(tǒng)計學差異;骨質(zhì)疏松狀態(tài)下頜骨來源BMSCs后期的增殖能力明顯優(yōu)于股骨來源的BMSCs(P0.05);四種膜片的大網(wǎng)膜移植結(jié)果顯示體內(nèi)移植6w后在的TCP誘導下均可形成不均質(zhì)的礦化物沉積和纖維樣組織穿通,似牙周膜/牙骨質(zhì)復合體樣結(jié)構(gòu);去卵巢組和未去卵巢組頜骨來源BMSCs組較同等條件下股骨來源BMSCs再生礦化物能力強,牙骨質(zhì)/牙周膜樣復合體的形成更滿意。結(jié)論骨質(zhì)疏松對BMSCs的增殖和分化可能存在著部位因素影響且具有一定的時間依賴性。
[Abstract]:Objective to observe the difference of proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) derived from mandible and femur of ovariectomized rats. Methods Osteoporosis model was established by bilateral ovariectomy in female SD rats. Bone marrow mesenchymal stem cells derived from rat jaws and femur were cultured under normal and osteoporosis condition. The cell morphology was observed and the proliferative ability of stem cells in four groups was compared by MTT and CCK-8. Four groups of stem cell membranes were prepared and combined with TCP for omentum transplantation. Histological observation was made 6 weeks after operation. Results compared with the unovariectomized group, the initial proliferation rate of BMSCs in ovariectomized group was lower than that in the healthy group after 3 days (P0.05), but there was no significant difference in the initial proliferation rate between the ovariectomized group and the non-ovariectomized group. There was no significant difference in OD value between jaw bone and femur BMSCs in healthy bone environment. The proliferative ability of mandibular BMSCs in the later stage of osteoporosis was significantly better than that of BMSCs derived from femur (P0.05). The results of four kinds of membrane transplantation of greater omentum showed that under the induction of TCP in vivo, uneven deposition of mineralized matter and perforation of fibrous tissue could be formed after 6 weeks of transplantation in vivo. Ovariectomized and non-ovariectomized BMSCs groups had stronger ability to regenerate mineralized matter than femur derived BMSCs, and the formation of cementum / periodontal membrane-like complex was more satisfactory than that of femur origin. Conclusion Osteoporosis may affect the proliferation and differentiation of BMSCs in a time dependent manner.
【作者單位】： 武警工程大學武警醫(yī)院急診科;武警工程大學武警醫(yī)院口腔中心;西安市第九人民醫(yī)院口腔科;
【基金】：武警后勤科研項目(wjh20110256)
【分類號】：R580
，

本文編號：2196373