【摘要】：目的觀察閾值下577 nm微脈沖激光光凝對早期糖尿病大鼠視網(wǎng)膜血管內皮生長因子(vascular endothelial growth factor,VEGF)、神經(jīng)生長因子(nerve growth factor,NGF)和趨化素(Chemerin)表達的影響。方法采用65 mg·kg~(-1)鏈脲佐菌素對40只Brown Norway大鼠建立糖尿病大鼠模型,選取20只造模成功者為實驗鼠,造模后2周右眼采用IQ577 nm激光行閾值下微脈沖激光處理為微脈沖激光組,左眼不做任何處理為對照組。在微脈沖激光光凝后3 d、7 d、14 d、28 d隨機各選取5只大鼠,應用實時熒光定量PCR和Western blot方法檢測大鼠雙眼視網(wǎng)膜VEGF、NGF和Chemerin表達情況。結果對照組VEGF mRNA和蛋白在3 d、7 d、14 d、28 d表達均增加,差異均有統(tǒng)計學意義(均為P0.05);微脈沖激光組VEGF mRNA和蛋白在3 d、7 d、14 d、28 d均較對照組下降,差異l 均有統(tǒng)計學意義(均為P0.05)。對照組3 d、7 d、14 d、28 d NGF mRNA和蛋白均逐漸下降(均為P0.05),7 d NGF比3 d下降,但差異無統(tǒng)計學意義(P0.05);微脈沖激光組NGF mRNA和蛋白在3 d、7 d、14 d、28 d均較對照組上升,且在14 d達到峰值,差異均有統(tǒng)計學意義(均為P0.05)。對照組Chemerin mRNA和蛋白在3 d、7 d、14 d、28 d表達均增加,差異均有統(tǒng)計學意義(均為P0.05);微脈沖激光組Chemerin mRNA和蛋白在3 d、7 d、14 d、28 d均較對照組下降,差異均有統(tǒng)計學意義(均為P0.05)。結論閾值下577 nm微脈沖激光光凝可降低早期糖尿病大鼠VEGF、Chemerin表達,上調NGF表達。
[Abstract]:Objective to investigate the effects of 577nm micropulse laser photocoagulation on the expression of vascular endothelial growth factor (vascular endothelial growth), nerve growth factor (nerve growth) and chemokine (Chemerin) in retina of early diabetic rats. Methods 40 Brown Norway rats were induced by 65 mg kg-1 streptozotocin, and 20 successful rats were selected as experimental rats. The right eye was treated with IQ577 nm laser at threshold for 2 weeks. The left eye was not treated as control group. Five rats were randomly selected at 3 days, 7 days, 14 days and 28 days after laser photocoagulation. The expression of VEGF and Chemerin in the binocular retina of rats was detected by real-time fluorescence quantitative PCR and Western blot. Results the expression of VEGF mRNA and protein in the control group increased on the 3rd day, 7th day, 14th day, the difference was statistically significant (P0.05), and the VEGF mRNA and protein in the micro-pulse laser group decreased at the 14th day of the 3rd day and the 14th day of the control group, the difference was statistically significant (P0.05). In the control group, the d NGF mRNA and protein decreased gradually (P0.05) on the 3rd day, the NGF at 7 days was lower than that on the third day (P0.05), but the difference was not significant (P0.05), the NGF mRNA and protein in the micropulse laser group were higher than those in the control group on the 3rd day after 7 days and the protein on the 14th day, and reached the peak value on the 14th day. The difference was statistically significant (P0.05). In the control group, the expression of Chemerin mRNA and protein increased on the 3rd day, 7th day and 14th day, the difference was statistically significant (P0.05), while the Chemerin mRNA and protein in the micro-pulse laser group decreased on the 14th day after 3 days, which were significantly lower than those in the control group (P0.05). Conclusion 577nm micro-pulse laser photocoagulation can decrease the expression of VEGF Chemerin and up-regulate the expression of NGF in early diabetic rats.
【作者單位】： 廣州軍區(qū)武漢總醫(yī)院;南昌大學附屬眼科醫(yī)院;
【基金】：國家自然科學基金資助(編號:61378084)~~
【分類號】：R587.2;R774

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