【摘要】：目的探討高原低氧條件下大鼠鐵代謝及其相關(guān)因子表達(dá)變化。方法選取雄性SD大鼠50只,將大鼠分別在低氧環(huán)境[低壓氧艙(模擬海拔5 000 m)飼養(yǎng)]下飼養(yǎng)7、15、30 d(低氧7 d、15 d、30 d組),常氧條件下[西寧地區(qū)(海拔2 260 m)飼養(yǎng)]飼養(yǎng)7、15、30 d(常氧7 d、15 d、30 d組)。分別于飼養(yǎng)7、15、30 d測(cè)定大鼠外周血血常規(guī)、血清鐵(SI)、血清鐵蛋白(SF)水平,qRT-PCR和Western blotting法測(cè)大鼠肝組織鐵調(diào)素(Hepc)、膜結(jié)合型鐵調(diào)素調(diào)節(jié)蛋白(m-HJV)、Ⅱ型跨膜絲氨酸蛋白酶(TMPRSS6)及肌肉組織可溶型鐵調(diào)素調(diào)節(jié)蛋白(s-HJV)mRNA和蛋白表達(dá)量,qRT-PCR和ELISA法測(cè)大鼠肝臟Hepc mRNA和蛋白表達(dá)量。結(jié)果低氧7 d、15 d、30 d組大鼠血紅蛋白(Hb)、紅細(xì)胞壓積(HCT)、SF水平均高于常氧同時(shí)間組(P均0.01),低氧30 d組SI水平高于常氧同時(shí)間組(P0.05),隨著缺氧時(shí)間延長(zhǎng)Hb、HCT、SI、SF水平逐漸升高(P均0.05)。低氧15 d、30 d組肝組織Hepc mRNA及蛋白表達(dá)量均低于常氧同時(shí)間組(P0.01或0.05),且隨著缺氧時(shí)間延長(zhǎng)其表達(dá)水平逐漸降低(P均0.01)。低氧各組間組大鼠肝組織m-HJV mRNA及蛋白表達(dá)量均低于常氧同時(shí)間組(P均0.01),隨缺氧時(shí)間延長(zhǎng)m-HJV逐漸降低(P均0.01)。低氧各時(shí)間組大鼠肌肉組織s-HJV mRNA及蛋白表達(dá)量、肝組織TMPRSS6 mRNA及蛋白表達(dá)量均高于常氧同時(shí)間組(P均0.01),且隨著缺氧時(shí)間延長(zhǎng)表達(dá)量逐漸升高(P均0.01)。結(jié)論高原低氧條件下,大鼠肝組織TMPRSS6表達(dá)量升高,可能裂解m-HJV成為s-HJV,使Hepc轉(zhuǎn)錄受抑制,最終導(dǎo)致SI水平升高。
[Abstract]:Objective to investigate the changes of iron metabolism and related factors in rats under high altitude hypoxia. Methods 50 male SD rats were selected. The rats were fed in hypoxic environment [hypobaric oxygen chamber (simulated altitude 5 000 m)] for 7 1515 days (hypoxia for 15 days or 30 days) and under normoxic [Xining area (2 260 m above sea level) for 7 days for 15 days 30 days). The blood routine in peripheral blood of rats was measured at 15 ~ 30 days after feeding. Serum iron (SI), serum ferritin (SF) levels were measured by qRT-PCR and Western blotting method for the determination of irontin (Hepc), membrane-binding iron-regulatory protein (m-HJV), transmembrane serine protease 鈪，

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