中鏈脂肪酸誘導(dǎo)的棕色脂肪分化和增殖及其在肥胖控制中的作用
發(fā)布時間：2018-07-23 07:41
【摘要】：目的1.觀察含6種含中鏈脂肪酸的食用油對肥胖小鼠體脂肪及血脂的影響。2.觀察中鏈脂肪酸對棕色脂肪剔除的小鼠體脂肪及血脂的影響。3.觀察中鏈脂肪酸對肥胖小鼠棕色脂肪增殖分化的影響。方法1.選取肥胖小鼠105只,隨機(jī)分為7組,每組15只,分別喂飼不同中鏈脂肪酸含量的飼料(6種)和大豆油飼料,喂飼16周。觀察每組小鼠體質(zhì)量、體脂肪、Lee氏指數(shù)、血脂、肝臟脂肪浸潤情況、白色脂肪細(xì)胞脂滴大小與大豆油組的差異。2.選取剔除了棕色脂肪的肥胖小鼠30只,隨機(jī)分為2組,每組15只,分別喂飼MCT飼料和LCT飼料,喂飼12周。觀察兩組小鼠體質(zhì)量、體脂肪、血脂、肝臟脂肪浸潤情況、白色脂肪細(xì)胞脂滴大小以及腹部皮下脂肪中UCP1 mRNA表達(dá)的變化。3.選取肥胖小鼠30只,隨機(jī)分為2組,每組15只,分別喂飼MCT飼料和LCT飼料,喂飼12周。觀察兩組小鼠體質(zhì)量、體脂肪、血脂、棕色脂肪micro-PET顯像、腹部皮下脂肪及棕色脂肪中 UCP1、BMP7、CIDEA、PRDM16、Cebp-alpha、Cebp-beta、Dio2、Fabp1、Fabp3、 Fabp4、FOXC2、Gyk、PPAR-alpha、PPAR-gamma、PGC1-alpha、PGC1-beta、COX2的mRNA及蛋白表達(dá)水平的變化。結(jié)果1.干預(yù)喂養(yǎng)16周后,6組喂飼含中鏈脂肪酸食用油的小鼠的體質(zhì)量、體脂肪量、Lee氏指數(shù)、血甘油三酯及總膽固醇濃度均顯著低于大豆油對照組(P0.05),肝臟組織的脂肪浸潤程度以及白色脂肪細(xì)胞的脂滴面積均小于大豆油組(P0.05)。2.剔除了棕色脂肪的肥胖小鼠,喂飼MCT和LCT飼料12周,未觀察到兩組小鼠體質(zhì)量、體脂肪、血脂、肝臟脂肪浸潤情況及白色脂肪細(xì)胞脂滴大小的顯著差異(P0.05),但MCT組小鼠腹部皮下脂肪中UCP1 mRNA表達(dá)明顯高于LCT組(P0.05)。3.干預(yù)喂養(yǎng)12周后,MCT組小鼠的體重、體脂肪量及血甘油三酯水平均顯著低于LCT組(P0.05), MCT組棕色脂肪重量顯著高于LCT組(P0.05)。Micro-PET成像顯示MCT組棕色脂肪體積及代謝活性均明顯高于LCT組。MCT組棕色脂肪組織中UCP1、CIDEA、Fabp4、PPAR-gamma、PRDM16、BMP7 的 mRNA 及蛋白表達(dá)水平均顯著高于 LCT 組(P0.05)。MCT 組棕色脂肪組織中 PGC1-alpha、FOXC2、COX2、Dio2、PGC1-beta、PPAR-alpha、CEBP-alpha、CEBP-beta 的 mRNA 表達(dá)水平均顯著高于 LCT組(P0.05)。MCT 組腹部皮下脂肪中 UCP1、CIDEA、Fabp4、PGC1-alpha、PPAR-gamma、PRDM16、FOXC2、BMP7的mRNA及蛋白表達(dá)水平均顯著高于LCT組(P0.05)。MCT 組腹部皮下脂肪中 COX2、Fabp1、Fabp3、Dio2、Gyk、PGC1-beta、PPAR-alpha、CEBP-alpha、CEBP-beta 的 mRNA 表達(dá)水平均顯著高于 LCT 組(P0.05)。結(jié)論1.中鏈脂肪酸的含量在10%～16%之間且辛酸癸酸的比例在2: 1～3: 1之間的食用油可有效降低肥胖小鼠體重、減少體脂蓄積、改善血脂。2.棕色脂肪是中鏈脂肪酸降脂減重作用的重要靶器官。3.中鏈脂肪酸可促進(jìn)棕色脂肪的分化、增殖及功能的增強(qiáng)以及促進(jìn)白色脂肪的棕色化。
[Abstract]:Objective 1. to observe the effect of 6 kinds of edible oil containing medium chain fatty acids on body fat and blood lipid in obese mice. The effect of chain fatty acid on body fat and blood lipid in mice with brown fat rejection in.2.. The effect of chain fatty acid on the proliferation and differentiation of brown fat in obese mice was observed in.3.. Method 1. 105 obese mice were randomly divided into 7 groups. 15 rats in each group were fed with different medium chain fatty acid content (6 kinds) and soybean oil feed for 16 weeks. The body mass, body fat, Lee's index, blood lipid, liver fatty infiltration, and the difference between the size of white fat cells and the soybean oil group were observed in each group, and 30 obese mice were selected to reject brown fat, and were randomly divided into 2 groups, which were randomly divided into 2 15 rats in each group were fed with MCT feed and LCT feed and fed for 12 weeks. The body mass of the two groups of mice, body fat, blood lipid, liver fatty infiltration, the size of white adipocyte lipid droplets and the change of UCP1 mRNA expression in the abdominal subcutaneous fat were selected, and 30 obese mice were selected and randomly divided into 2 groups, 15 rats in each group were fed with MCT feed and LCT feeding respectively. Material, feed for 12 weeks. Observe the body mass of two groups of mice, body fat, lipid, brown fat micro-PET imaging, abdominal subcutaneous fat and brown fat in UCP1, BMP7, CIDEA, PRDM16, Cebp-alpha, Cebp-beta, Dio2, Fabp1, Fabp3, Fabp4, FOXC2, Fabp3, and protein expression level changes. Results 1. After 16 weeks of intervention feeding, the body mass, body fat volume, Lee's index, triglyceride and total cholesterol in the 6 groups were significantly lower than that of the control group (P0.05), the degree of fatty infiltration of the liver tissue and the area of white fat cells were less than that of the soybean oil group (P0.05).2. eliminated the brown. The obese mice were fed with MCT and LCT for 12 weeks. The body mass of the two groups, body fat, blood lipid, liver fatty infiltration and the size of white fat cells were significantly different (P0.05), but the expression of UCP1 mRNA in the abdominal subcutaneous fat of group MCT mice was significantly higher than that of the LCT group (P0.05).3. intervention for 12 weeks, and MCT mice in the MCT group Weight, body fat and triglyceride were significantly lower than group LCT (P0.05), and the weight of brown fat in group MCT was significantly higher than that in group LCT (P0.05).Micro-PET imaging showed that the volume and metabolic activity of brown fat in group MCT was significantly higher than that in group.MCT in group LCT.MCT UCP1, CIDEA, Fabp4, and protein expression water. The average levels of PGC1-alpha, FOXC2, COX2, Dio2, PGC1-beta, PPAR-alpha, CEBP-alpha, CEBP-beta in the brown adipose tissue of group LCT (P0.05).MCT were significantly higher than those in the abdominal subcutaneous fat. The levels of COX2, Fabp1, Fabp3, Dio2, Gyk, PGC1-beta, PPAR-alpha, CEBP-alpha, in the abdominal subcutaneous fat of group LCT (P0.05).MCT were significantly higher than those in the group. Conclusion the content of fatty acids in the 1. chain is between 10% and 16% and the proportion of octanoic acid decanoic acid between 1 and 1 can be effectively reduced. Weight of fat mice, reducing body fat accumulation, improving blood lipid.2. brown fat is an important target organ for reducing fat and weight loss of medium chain fatty acids,.3. of chain fatty acids can promote the differentiation of brown fat, increase the proliferation and function and promote the browning of white fat.
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：R589.2
本文編號：2138680
[Abstract]:Objective 1. to observe the effect of 6 kinds of edible oil containing medium chain fatty acids on body fat and blood lipid in obese mice. The effect of chain fatty acid on body fat and blood lipid in mice with brown fat rejection in.2.. The effect of chain fatty acid on the proliferation and differentiation of brown fat in obese mice was observed in.3.. Method 1. 105 obese mice were randomly divided into 7 groups. 15 rats in each group were fed with different medium chain fatty acid content (6 kinds) and soybean oil feed for 16 weeks. The body mass, body fat, Lee's index, blood lipid, liver fatty infiltration, and the difference between the size of white fat cells and the soybean oil group were observed in each group, and 30 obese mice were selected to reject brown fat, and were randomly divided into 2 groups, which were randomly divided into 2 15 rats in each group were fed with MCT feed and LCT feed and fed for 12 weeks. The body mass of the two groups of mice, body fat, blood lipid, liver fatty infiltration, the size of white adipocyte lipid droplets and the change of UCP1 mRNA expression in the abdominal subcutaneous fat were selected, and 30 obese mice were selected and randomly divided into 2 groups, 15 rats in each group were fed with MCT feed and LCT feeding respectively. Material, feed for 12 weeks. Observe the body mass of two groups of mice, body fat, lipid, brown fat micro-PET imaging, abdominal subcutaneous fat and brown fat in UCP1, BMP7, CIDEA, PRDM16, Cebp-alpha, Cebp-beta, Dio2, Fabp1, Fabp3, Fabp4, FOXC2, Fabp3, and protein expression level changes. Results 1. After 16 weeks of intervention feeding, the body mass, body fat volume, Lee's index, triglyceride and total cholesterol in the 6 groups were significantly lower than that of the control group (P0.05), the degree of fatty infiltration of the liver tissue and the area of white fat cells were less than that of the soybean oil group (P0.05).2. eliminated the brown. The obese mice were fed with MCT and LCT for 12 weeks. The body mass of the two groups, body fat, blood lipid, liver fatty infiltration and the size of white fat cells were significantly different (P0.05), but the expression of UCP1 mRNA in the abdominal subcutaneous fat of group MCT mice was significantly higher than that of the LCT group (P0.05).3. intervention for 12 weeks, and MCT mice in the MCT group Weight, body fat and triglyceride were significantly lower than group LCT (P0.05), and the weight of brown fat in group MCT was significantly higher than that in group LCT (P0.05).Micro-PET imaging showed that the volume and metabolic activity of brown fat in group MCT was significantly higher than that in group.MCT in group LCT.MCT UCP1, CIDEA, Fabp4, and protein expression water. The average levels of PGC1-alpha, FOXC2, COX2, Dio2, PGC1-beta, PPAR-alpha, CEBP-alpha, CEBP-beta in the brown adipose tissue of group LCT (P0.05).MCT were significantly higher than those in the abdominal subcutaneous fat. The levels of COX2, Fabp1, Fabp3, Dio2, Gyk, PGC1-beta, PPAR-alpha, CEBP-alpha, in the abdominal subcutaneous fat of group LCT (P0.05).MCT were significantly higher than those in the group. Conclusion the content of fatty acids in the 1. chain is between 10% and 16% and the proportion of octanoic acid decanoic acid between 1 and 1 can be effectively reduced. Weight of fat mice, reducing body fat accumulation, improving blood lipid.2. brown fat is an important target organ for reducing fat and weight loss of medium chain fatty acids,.3. of chain fatty acids can promote the differentiation of brown fat, increase the proliferation and function and promote the browning of white fat.
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：R589.2
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 WU LiZhen;ZHOU LinKang;CHEN Cheng;GONG JingYi;XU Li;YE Jing;LI De;LI Peng;;Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue[J];Science China(Life Sciences);2014年01期
，本文編號：2138680
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