本文選題：2型糖尿病 + 新疆維吾爾族��； 參考：《石河子大學(xué)》2015年碩士論文

【摘要】：目的:采用逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)及基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜(MALDI-TOF-MS)技術(shù)等探討FOSL2基因表達及其DNA甲基化情況在新疆維吾爾族2型糖尿病(T2DM)發(fā)病機制中的作用。方法:1.收集2013年09月-2014年03月間新疆維吾爾自治區(qū)喀什地區(qū)莎車縣維吾爾族T2DM與維吾爾族非DM患者病例各50例,測定記錄其一般資料,包括性別、年齡、身高、體重、腰圍、臀圍、血壓、糖尿病病程等一般臨床基線資料,根據(jù)公式計算出腰臀比(WHR)、體重指數(shù)(BMI)。采集研究對象空腹靜脈血標(biāo)本經(jīng)肝素抗凝,使用全自動生化分析儀測定空腹血糖(FPG)、血清膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白膽固醇(LDL-C)、高密度脂蛋白膽固醇(HDL)等生化指標(biāo),高壓液相法測定糖化血紅蛋白(Hb A1c);2.采用RT-PCR法檢測FOSL2基因m RNA表達水平;3.采用MALDI-TOF MS法檢測外周血白細胞FOSL2基因DNA甲基化水平;4.采用SPSS17.0統(tǒng)計軟件進行數(shù)據(jù)處理。計量資料用均數(shù)±標(biāo)準(zhǔn)差表示,兩組間均數(shù)比較采用t檢驗。兩組間甲基化率比較運用兩個獨立樣本的Wilcoxon秩和檢,變量之間的相關(guān)關(guān)系采用多元線性回歸分析,,以P0.05為差異有統(tǒng)計學(xué)意義。結(jié)果:1.與新疆維吾爾族非DM組相比,新疆維吾爾族T2DM組在年齡、性別比、收縮壓(SBP)、舒張壓(DBP)、WHR等一般基線資料無統(tǒng)計學(xué)差異(P0.05),說明基線齊,資料具有可比性;2.與新疆維吾爾族非DM組相比,新疆維吾爾族T2DM組患者FOSL2基因m RNA表達水平明顯降低(2.456±0.512,0.996±0.159;P0.01);3.與新疆維吾爾族非DM病組相比,新疆維吾爾族T2DM組患者FOSL2基因CPG Unit3(CPG_3)(0.016±0.004,0.030±0.003;P0.01)、CPG Unit4(CPG_4.5)(0.065±0.006,0.095±0.007;P0.05)、CPG Unit5(CPG_6.7)(0.002±0.001,0.014±0.002;P0.01)、CPG Unit6(CPG_8)(0.004±0.001,0.023±0.003;P0.01)、CPG Unit8(CPG_11)(0.002±0.001,0.029±0.003;P0.01)、CPG Unit9(CPG_12.13.14)(0.092±0.004,0.119±0.003;P0.01)、CPG Unit10(CPG_15.16.17)(0.008±0.002,0.019±0.001;P0.01)DNA的甲基化率均較高;4.在新疆維吾爾族T2DM組中,以FOSL2基因DNA平均甲基化率為應(yīng)變量,患者的年齡、身高、體重、腰圍、臀圍、WHR、BMI、脈率、SBP、DBP、FPG、Hb A1c、TC、TG、LDL、HDL等指標(biāo)為自變量進行多元線性回歸分析,結(jié)果顯示患者身高、體重、BMI、脈率、SBP、FPG、Hb A1c進入回歸方程�；貧w方程:y=-1.521+0.007(身高)-0.006(體重)+0.018(BMI)+0.001(脈率)+0.001(SBP)-0.003(FPG)+0.004(Hb A1c)。結(jié)論:1.FOSL2基因可能與新疆維吾爾族T2DM、肥胖及糖脂代謝的異常相關(guān)。2.FOSL2基因DNA多個位點的高甲基化可能致使其m RNA表達水平的下調(diào),參與新疆維吾爾族T2DM的發(fā)生與發(fā)展。
[Abstract]:Objective: to investigate the role of FOSL2 gene expression and DNA methylation in the pathogenesis of type 2 diabetes mellitus (T2DM) in Xinjiang Uygur nationality by reverse transcription polymerase chain reaction (RT-PCR) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Method 1: 1. From September 2013 to March 2014, 50 Uygur patients with T2DM and 50 Uygur non-DM patients were collected from Shacha County, Kashi County, Xinjiang Uygur Autonomous region, and their general data including gender, age, height, weight, waist circumference and hip circumference were measured and recorded, including sex, age, height, weight, waist circumference and hip circumference. The general clinical baseline data such as blood pressure, diabetes course and so on. WHR and BMI were calculated according to the formula. Fasting venous blood samples were collected by heparin anticoagulant. Fasting blood glucose (FPG), serum cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL) were measured by automatic biochemical analyzer. Determination of glycosylated hemoglobin (HB A 1c) by high pressure liquid chromatography. The mRNA expression level of FOSL2 gene was detected by RT-PCR. The DNA methylation level of FOSL2 gene in peripheral blood leukocytes was detected by MALDI-TOF MS. SPSS 17.0 statistical software was used to process the data. The measurement data were expressed as mean 鹵standard deviation. T test was used to compare the mean between the two groups. The methylation rate of the two groups was compared by Wilcoxon rank sum test of two independent samples and the correlation between the variables was analyzed by multiple linear regression analysis. The difference was statistically significant with P0.05. The result is 1: 1. There was no statistical difference in age, sex ratio, SBP, DBP and WHR between Xinjiang Uygur T2DM group and Xinjiang Uygur non-DM group (P0.05). Compared with non-DM group, the mRNA expression level of FOSL2 gene in Uygur T2DM group was significantly lower than that in non-DM group of Xinjiang Uygur nationality (2.456 鹵0.512C0.996 鹵0.159 P0.01). 涓庢柊鐤嗙淮鍚懼皵鏃忛潪DM鐥呯粍鐩告瘮,鏂扮枂緇村惥灝?dāng)鏃廡2DM緇勬?zhèn)ｈ�匜OSL2鍩哄洜CPG Unit3(CPG_3)(0.016鹵0.004,0.030鹵0.003;P0.01),CPG Unit4(CPG_4.5)(0.065鹵0.006,0.095鹵0.007;P0.05),CPG Unit5(CPG_6.7)(0.002鹵0.001,0.014鹵0.002;P0.01),CPG Unit6(CPG_8)(0.004鹵0.001,0.023鹵0.003;P0.01),CPG Unit8(CPG_11)(0.002鹵0.001,0.029鹵0.003;P0.01),CPG Unit9(CPG_12.13.14)(0.092鹵0.004,0.119鹵0.003;P0.01),CPG Unit10(CPG_15.16.17)(0.008鹵0.002,0.019鹵0.001;P0.01)DNA鐨勭敳鍩哄寲鐜囧潎杈冮珮;4. In Xinjiang Uygur T2DM group, the average DNA methylation rate of FOSL2 gene was taken as the dependent variable, the age, height, weight, waist circumference, hip circumference of WHRBMIand pulse rate of SBPNDBPFPPFPPFPGFG, HbA1cTCnTCnTGG / LDLHDL were used as independent variables. The results showed that the patients' height, body weight, waist circumference and hip circumference were determined by multivariate linear regression analysis, and the results showed that the patients' height, weight, waist circumference, hip circumference, etc. Body weight, BMI, pulse rate, SBP, FPGG, HB A 1c entered the regression equation. The regression equation was: yyyong -1.521 0.007 (height) -0.006 (body weight) 0.018 (BMI) 0.001 (pulse rate) 0.001 (SBP) -0.003 (FPG) 0.004 (HbA1c). Conclusion: 1. FOSL2 gene may be associated with T2DM. obesity and abnormal metabolism of glucose and lipid. 2. Hypermethylation of DNA of FOSL2 gene may lead to down-regulation of mRNA expression level, and participate in the occurrence and development of T2DM in Xinjiang Uygur nationality.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R587.1

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