本文選題：連續(xù)運(yùn)動(dòng) + 間斷運(yùn)動(dòng)　； 參考：《寧夏醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的本實(shí)驗(yàn)以高脂飲食肥胖大鼠為模型,通過連續(xù)和間斷運(yùn)動(dòng)(continuous and intermittent exercise)干預(yù),觀察連續(xù)和間斷運(yùn)動(dòng)對(duì)高脂飲食肥胖大鼠胸主動(dòng)脈血管反應(yīng)性的影響,以期初步闡明高脂飲食中引起心血管功能損傷的可能機(jī)制,也為進(jìn)一步開發(fā)連續(xù)和間斷運(yùn)動(dòng)提供實(shí)驗(yàn)依據(jù)和理論指導(dǎo)。方法將雄性SD大鼠48只,隨機(jī)分成6組,普通飲食組(conventional diet,CD)、普通飲食連續(xù)運(yùn)動(dòng)組(conventional with continuous exercise group,CCE)、普通飲食間斷運(yùn)動(dòng)組(conventional with intermittent exercise group,CIE)、高脂飲食組(high-fat diet,HD)、高脂飲食連續(xù)運(yùn)動(dòng)組(high-fat diet with continuous exercise,HCE)、高脂飲食間斷運(yùn)動(dòng)組(high-fat diet with intermittent exercise,HIE)。進(jìn)行8周游泳訓(xùn)練后,觀察大鼠體重、食物的利用率、Lee’s指數(shù)、體長的變化;采用血管環(huán)組織浴槽離體技術(shù)測定去甲腎上腺素(Noradrenaline,NA)對(duì)大鼠胸主動(dòng)脈血管的反應(yīng)性;檢測血清中超氧化物歧化酶(Super oxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、一氧化氮(Nitric oxide,NO)的變化,低密度脂蛋白(low density lipoprotein,LDL)、膽固醇(Cholesterol,CHOL)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(High-density lipoprotein,HDL);檢測大鼠血管胸主動(dòng)脈一氧化氮合酶(Endothelial nitric oxide synthase,e NOS)基因的表達(dá)水平;觀察胸主動(dòng)脈形態(tài)學(xué)變化。結(jié)果1.各組大鼠體重均逐周增長,均有統(tǒng)計(jì)學(xué)意義(P0.01)。2.各組大鼠體長均逐漸增長,與普通飲食組比較,高脂飲食組體長略長一些(P0.05),而普通飲食連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組無統(tǒng)計(jì)學(xué)意義(P0.05);與高脂飲食組比較,高脂飲食間斷運(yùn)動(dòng)組體長略小一些(P0.05),高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組比較,無統(tǒng)計(jì)學(xué)意義(P0.05)。3.高脂飲食組大鼠食物利用率顯著高于普通飲食組(P0.01),普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組食物利用率顯著低于高脂飲食組(P0.01),高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組食物利用率低于高脂飲食組(P0.01);而連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組比較,無統(tǒng)計(jì)學(xué)意義(P0.05)。4.高脂飲食組LEE’S指數(shù)明顯高于普通飲食組(P0.01),普通飲食連續(xù)運(yùn)動(dòng)和間斷動(dòng)和間斷運(yùn)動(dòng)組LEE’S指數(shù)低于高脂飲食組(P0.01),但高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組LEE’S指數(shù)比較,無統(tǒng)計(jì)學(xué)差異(P0.05)。5.高脂飲食組大鼠胸主動(dòng)脈血管環(huán)對(duì)NA的收縮反應(yīng)高于普通飲食組(P0.01);與高脂飲食組相比,高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組對(duì)NA的收縮反應(yīng)有所降低,有顯著性差異(P0.01),與高脂飲食連續(xù)運(yùn)動(dòng)組比較,高脂飲食間斷運(yùn)動(dòng)組對(duì)NA的收縮反應(yīng)降低更明顯(P0.01);與普通飲食組比較,普通飲食連續(xù)運(yùn)動(dòng)組和普通飲食間斷運(yùn)動(dòng)組也有所下降(P0.01),而與普通飲食連續(xù)運(yùn)動(dòng)組比較,普通飲食間斷運(yùn)動(dòng)組略下降(P0.05)。6.普通飲食連續(xù)運(yùn)動(dòng)組和普通飲食間斷運(yùn)動(dòng)組血清中CHOL、TG、LDL含量低于普通飲食組(P0.05),高脂飲食組血清中CHOL、TG、LDL含量高于普通飲食組(P0.01),高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組CHOL、TG、LDL低于高脂飲食組(P0.01),間斷運(yùn)動(dòng)組血清中CHOL、TG、LDL含量低于連續(xù)運(yùn)動(dòng)組(P0.01);高脂飲食組血清中HDL的含量低于普通飲食組,無統(tǒng)計(jì)學(xué)意義(P0.05),普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組血清中HDL的含量略高于普通飲食組,無統(tǒng)計(jì)學(xué)意義(P0.05);而高脂飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組HDL含量高于高脂飲食組(P0.01),間斷運(yùn)動(dòng)組和連續(xù)運(yùn)動(dòng)組HDL含量比較并無統(tǒng)計(jì)學(xué)差異(P0.05)。與普通飲食組比較,高脂飲食組NO含量減少(P0.01),普通飲食連續(xù)和間斷運(yùn)動(dòng)組NO含量并無明顯變化(P0.05);與高脂飲食組比較,高脂飲食連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組NO含量略上升,但無統(tǒng)計(jì)學(xué)意義(P0.05)。7.高脂飲食組心肌中MDA含量高于普通飲食組(P0.01),普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組心肌中MDA含量低于普通飲食組(P0.01),而普通飲食間斷運(yùn)動(dòng)組心肌中MDA含量比普通飲食連續(xù)運(yùn)動(dòng)組下降得更明顯(P0.05);高脂飲食連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組心肌中MDA含量低于高脂飲食組(P0.01),而高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組并無差異(P0.05)。與普通飲食組比較,高脂飲食組心肌中SOD含量下降(P0.01),普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組心肌中SOD含量上升(P0.01),普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組比較并無統(tǒng)計(jì)學(xué)差異(P0.05);和高脂飲食組比較,高脂飲食連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組心肌中SOD含量上升(P0.01),而高脂飲食連續(xù)運(yùn)動(dòng)組和高脂飲食間斷運(yùn)動(dòng)組比較也無統(tǒng)計(jì)學(xué)差異(P0.05)。8.普通飲食組eNOS mRNA基因表達(dá)水平高于高脂飲食組(P0.05),與普通飲食組相比,普通飲食連續(xù)運(yùn)動(dòng)組和間斷運(yùn)動(dòng)組胸主動(dòng)脈血管e NOS m RNA基因表達(dá)有上調(diào)趨勢,但無統(tǒng)計(jì)學(xué)意義(P0.05);普通飲食連續(xù)運(yùn)動(dòng)組和普通飲食間斷運(yùn)動(dòng)組比較無統(tǒng)計(jì)學(xué)差異(P0.05);高脂飲食連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)組中胸主動(dòng)脈血管e NOS m RNA基因的表達(dá)量顯著高于高脂飲食組(P0.05),而高脂飲食間斷運(yùn)動(dòng)與高脂飲食連續(xù)運(yùn)動(dòng)組比較無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論連續(xù)運(yùn)動(dòng)和間斷運(yùn)動(dòng)具有改善高脂飲食肥胖大鼠動(dòng)脈血管反應(yīng)性的作用;增加血清抗氧化酶活力,減少心肌自由基的代謝及改善血清脂質(zhì)代謝,使e NOS m RNA的表達(dá)上調(diào),增加NO含量,間斷運(yùn)動(dòng)方式總體來說改善的更好。
[Abstract]:Objective To observe the effect of continuous and intermittent movement (continuous and intermittent exercise) on the vascular reactivity of thoracic aorta in high fat diet obese rats by continuous and intermittent movement (intermittent exercise), and to clarify the possible mechanism of cardiovascular damage in high fat diet. The experimental basis and theoretical guidance were provided for further development of continuous and intermittent movement. Methods 48 male SD rats were randomly divided into 6 groups, ordinary diet group (conventional diet, CD), common diet continuous exercise group (conventional with continuous exercise group, CCE), common diet intermittent exercise group (conventional with) Group, CIE), the high fat diet group (high-fat diet, HD), the high fat diet continuous exercise group (high-fat diet with continuous exercise, HCE), and the high fat diet intermittent exercise group (high-fat). After swimming training, the body weight, the utilization rate of food, the change of body length, the use of blood vessels and the blood vessel were observed. The reactivity of Noradrenaline (NA) to the aorta of thoracic aorta in rats was measured in vitro. The changes in serum superoxide dismutase (Super oxide dismutase, SOD), malondialdehyde (Malondialdehyde, MDA), nitric oxide (Nitric oxide, NO), low density lipoprotein (low), gallbladder, and bile solid were detected. Alcohol (Cholesterol, CHOL), triglyceride (Triglyceride, TG), high density lipoprotein (High-density lipoprotein, HDL), the expression level of nitric oxide synthase (Endothelial nitric oxide synthase, e) gene in the thoracic aorta of rats and the morphological changes of the thoracic aorta were observed. Results 1. groups of rats were all body weight per week. In P0.01.2., the body length of rats in each group increased gradually. Compared with the normal diet group, the high fat diet group was slightly longer (P0.05), while the continuous exercise and the intermittent exercise group had no statistical significance (P0.05). Compared with the high fat diet group, the body length of the high fat diet group was slightly smaller (P0.05) and the high fat diet continuous exercise group was more than that of the high fat diet group. Compared with the high fat diet intermittent exercise group, the food utilization rate of the.3. high fat diet group was significantly higher than that of the normal diet group (P0.01). The food utilization rate of the continuous exercise group and the intermittent exercise group was significantly lower than that of the high fat diet group (P0.01). The food in the high fat diet continuous exercise group and the high fat diet intermittent exercise group were better than those in the high fat diet group (P0.01). The use rate was lower than the high fat diet group (P0.01), while the continuous exercise and intermittent exercise group had no statistical significance (P0.05) the LEE 'S index of the high fat diet group was significantly higher than that of the normal diet group (P0.01). The LEE' S index of the regular diet and discontinuous exercise group was lower than the high fat diet group (P0.01), but the high fat diet continuous exercise group and the high fat diet group were higher than the high fat diet group (P0.01), but the high fat diet group and the high fat diet group were higher than the high fat diet group (P0.01). There was no statistical difference in the LEE 'S index in the discontinuous exercise group (P0.05). The contraction response of the thoracic aorta ring to the NA was higher in the.5. high fat diet group than that of the normal diet group (P0.01). Compared with the high fat diet group, the contraction reaction of the high fat diet continuous exercise group and the high fat diet intermittent movement group was lower than the high fat diet group (P0). (P0 .01), compared with the high fat diet continuous exercise group, the contractile response of NA in the high fat diet group decreased significantly (P0.01). Compared with the ordinary diet group, the continuous exercise group and the normal diet discontinuous exercise group also decreased (P0.01), but compared with the regular diet exercise group, the normal diet intermittent exercise group decreased slightly (P0.05 The content of CHOL, TG, and LDL in the serum of the.6. diet continuous exercise group and the normal diet discontinuous exercise group was lower than that of the ordinary diet group (P0.05). The content of CHOL, TG, LDL in the serum of high fat diet group was higher than that of the ordinary diet group (P0.01). The high fat diet continuous exercise group and the high fat diet break exercise group were CHOL, TG, LDL was lower than the high fat diet group and intermittent exercise group. The content of CHOL, TG and LDL in the serum was lower than that of the continuous exercise group (P0.01), and the content of HDL in the serum of high fat diet group was lower than that of the ordinary diet group (P0.05). The content of HDL in the serum of the normal diet continuous exercise group and the intermittent exercise group was slightly higher than that of the ordinary diet group (P0.05), but the high fat diet continuous exercise group and the intermittent transport group were not statistically significant (P0.05). The content of HDL in the dynamic group was higher than that of the high fat diet group (P0.01), and there was no significant difference in the HDL content between the intermittent exercise group and the continuous exercise group (P0.05). Compared with the normal diet group, the NO content of the high fat diet group decreased (P0.01), and the NO content in the normal diet continuous and discontinuous exercise group had no obvious changes (P0.05), and the high fat diet was compared with the high fat diet group. The content of NO in the dynamic and intermittent exercise groups was slightly higher, but the content of MDA in the myocardium of the high fat diet group was higher than that of the ordinary diet group (P0.01). The content of MDA in the myocardium of the normal diet continuous exercise group and the intermittent exercise group was lower than that of the normal diet group (P0.01), while the content of MDA in the myocardium of the normal diet group was more than that of the normal diet group (P0.01). The content of MDA in the normal diet group was more than that of the normal diet group (P0.01). The MDA content in the myocardium of the high fat diet continuous exercise and the intermittent exercise group was lower than that of the high fat diet group (P0.01), but there was no difference between the high fat diet continuous exercise group and the high fat diet intermittent exercise group (P0.05). Compared with the normal diet group, the SOD content decreased (P0.01) in the high fat diet group (SOD) and the normal diet continuous exercise group. There was no significant difference in the SOD content in the myocardium in the intermittent exercise group (P0.01), and there was no statistical difference between the continuous exercise group and the intermittent exercise group (P0.05). Compared with the high fat diet group, the SOD content in the continuous and intermittent exercise groups of the high fat diet increased (P0.01), while the high fat diet continuous exercise group and the high fat diet intermittent exercise group were also compared. There was no statistical difference (P0.05).8. common diet group eNOS mRNA gene expression level is higher than the high fat diet group (P0.05), compared with the ordinary diet group, the common diet continuous exercise group and intermittent exercise group of thoracic aorta, e NOS m RNA gene expression up-regulated, but no statistical significance (P0.05); common diet continuous exercise group and ordinary diet. There was no statistical difference in the discontinuous exercise group (P0.05); the expression of the e NOS m RNA gene in the thoracic aorta in the high fat diet continuous exercise group and the intermittent exercise group was significantly higher than that in the high fat diet group (P0.05), while the high fat diet intermittent movement and the high fat diet continuous exercise group had no statistical difference (P0.05). Conclusion continuous exercise and intermittent exercise have no statistical difference (P0.05). Improve arterial responsiveness of rat with high fat diet effect; increase the antioxidant activity of serum, reduce myocardial free radical metabolism and improve serum lipid metabolism, increase the expression of RNA e NOS m, increase the content of NO, generally intermittent exercise better.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R589.2

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